Analytical and Bioanalytical Chemistry

, Volume 398, Issue 1, pp 425–434 | Cite as

Determination of organic acids in Vaccinium berry standard reference materials

  • Melissa M. Phillips
  • Ryan J. Case
  • Catherine A. Rimmer
  • Lane C. Sander
  • Katherine E. Sharpless
  • Stephen A. Wise
  • James H. Yen
Original Paper


Nine organic acids (citric acid, galacturonic acid, glycolic acid, isocitric acid, malic acid, oxalic acid, quinic acid, shikimic acid, and tartaric acid) and two anions (phosphate and sulfate) were determined in a suite of Vaccinium berry-containing dietary supplement standard reference materials (SRMs). Following solvent extraction, three independent methods were utilized in the quantification of these compounds. The first method involved reversed-phase liquid chromatography with ultraviolet absorbance detection at 210 nm and isotope dilution mass spectrometry. The second method utilized ion chromatography with conductivity detection. Finally, gas chromatography with isotope dilution mass spectrometry detection was used following derivatization with N-methyl-N-trifluoroacetamide (MSTFA). The combined data from these methods was used for the assignment of organic acid levels in the seven candidate SRMs.


Organic acids Berry Liquid chromatography Gas chromatography Ion chromatography Mass spectrometry Isotope dilution 



The development of this suite of seven Vaccinium berry SRMs is part of a collaborative effort with the National Institutes of Health Office of Dietary Supplements (NIH-ODS) and the Food and Drug Administration Center for Drug Evaluation and Research (FDA-CDER). The authors would like to acknowledge the assistance of Dr. Elizabeth McGaw (NIST) in GC-IDMS method development.


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Copyright information

© US Government 2010

Authors and Affiliations

  • Melissa M. Phillips
    • 1
  • Ryan J. Case
    • 1
  • Catherine A. Rimmer
    • 1
  • Lane C. Sander
    • 1
  • Katherine E. Sharpless
    • 1
  • Stephen A. Wise
    • 1
  • James H. Yen
    • 2
  1. 1.Analytical Chemistry Division, Chemical Science and Technology LaboratoryNational Institute of Standards and TechnologyGaithersburgUSA
  2. 2.Statistical Engineering Division, Information Technology LaboratoryNational Institute of Standards and TechnologyGaithersburgUSA

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