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Dynamics of hyaluronan aqueous solutions as assessed by fast field cycling NMR relaxometry

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Abstract

Fast field cycling (FFC) NMR relaxometry has been used to study the conformational properties of aqueous solutions of hyaluronan (HYA) at three concentrations in the range 10 to 25 mg mL–1. Results revealed that, irrespective of the solution concentration, three different hydration layers surround hyaluronan. The inner layer consists of water molecules strongly retained in the proximity of the HYA surface. Because of their strong interactions with HYA, water molecules in this inner hydration layer are subject to very slow dynamics and have the largest correlation times. The other two hydration layers are made of water molecules which are located progressively further from the HYA surface. As a result, decreasing correlation times caused by faster molecular motion were measured. The NMRD profiles obtained by FFC-NMR relaxometry also showed peaks attributable to 1H–14N quadrupole interactions. Changes in intensity and position of the quadrupolar peaks in the NMRD profiles suggested that with increasing concentration the amido group is progressively involved in the formation of weak and transient intramolecular water bridging adjacent hyaluronan chains. In this work, FFC-NMR was used for the first time to obtain deeper insight into HYA–water interactions and proved itself a powerful and promising tool in hyaluronan chemistry.

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Acknowledgments

This work was partially funded by Ce.R.T.A. s.c.r.l. (Centri Regionali per le Tecnologie Alimentari; http://www.certa.it/default.asp) and by the Ministry of Education, Youth and Sport of the Czech Republic, project no. 0021630501. A.P. acknowledges an Erasmus project which enabled her to work at the Università degli Studi di Palermo. The authors kindly acknowledge Dr. Vladimír Velebný (CPN company, Dolní Dobrouč, Czech Republic) for providing the hyaluronan sample.

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Authors and Affiliations

  1. Faculty of Chemistry, Brno University of Technology, Purkyňova 118, 612 00, Brno, Czech Republic

    Alena Průšová & Jiří Kučerík

  2. Dipartimento di Ingegneria e Tecnologie Agro-Forestali, Università degli Studi di Palermo, v.le delle Scienze 13, edificio 4, 90128, Palermo, Italy

    Pellegrino Conte & Giuseppe Alonzo

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  1. Alena Průšová
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  2. Pellegrino Conte
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  3. Jiří Kučerík
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  4. Giuseppe Alonzo
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Correspondence to Pellegrino Conte.

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Průšová, A., Conte, P., Kučerík, J. et al. Dynamics of hyaluronan aqueous solutions as assessed by fast field cycling NMR relaxometry. Anal Bioanal Chem 397, 3023–3028 (2010). https://doi.org/10.1007/s00216-010-3855-9

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  • DOI: https://doi.org/10.1007/s00216-010-3855-9

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