Analytical and Bioanalytical Chemistry

, Volume 397, Issue 5, pp 1743–1753 | Cite as

Celiac disease diagnosis and gluten-free food analytical control

  • Marta Maria Pereira da Silva Neves
  • Maria Begoña González-Garcia
  • Hendrikus Petrus Antonius Nouws
  • Cristina Delerue-Matos
  • Alice Santos-Silva
  • Agustín Costa-García
Review

Abstract

Celiac disease (CD) is an autoimmune enteropathy, characterized by an inappropriate T-cell-mediated immune response to the ingestion of certain dietary cereal proteins in genetically susceptible individuals. This disorder presents environmental, genetic, and immunological components. CD presents a prevalence of up to 1% in populations of European ancestry, yet a high percentage of cases remain underdiagnosed. The diagnosis and treatment should be made early since untreated disease causes growth retardation and atypical symptoms, like infertility or neurological disorders. The diagnostic criteria for CD, which requires endoscopy with small bowel biopsy, have been changing over the last few decades, especially due to the advent of serological tests with higher sensitivity and specificity. The use of serological markers can be very useful to rule out clinical suspicious cases and also to help monitor the patients, after adherence to a gluten-free diet. Since the current treatment consists of a life-long gluten-free diet, which leads to significant clinical and histological improvement, the standardization of an assay to assess in an unequivocal way gluten in gluten-free foodstuff is of major importance.

Keywords

Celiac disease Autoimmune Transglutaminase Gliadin 

Abbreviations

AGA

anti-gliadin antibodies

CD

celiac disease

EIs

electrochemical immunosensores

EMA

endomysial antibodies

ELISA

enzyme-linked immunosorbent assay

Ig

immunoglobulin

ROC

receiver operating characteristics

tTG

tissue transglutaminase

References

  1. 1.
    Holtmeier W, Caspary WF (2006) Celiac disease. Orphanet J Rare Dis 1:1–3Google Scholar
  2. 2.
    Goddard CJR, Gillett HR (2006) Complications of celiac disease: are all patients are at risk? Postgrad Med J 82(973):705–712Google Scholar
  3. 3.
    Abdulkarim AS, Murray JA (2003) Review article: the diagnosis of coeliac disease. Aliment Pharmacol Ther 17(8):987–995Google Scholar
  4. 4.
    Briani C, Samaroo D, Alaedini A (2008) Celiac disease: from gluten to autoimmunity. Autoimmun Rev 7(8):644–650Google Scholar
  5. 5.
    Ascher H, Krantz I, Kristiansson B (1991) Increasing incidence of coeliac disease in Sweden. Arch Dis Child 66(5):608–611Google Scholar
  6. 6.
    Catassi C, Fabiani E, Rätsch IM, Coppa GV, Giorgi PL, Pierdomenico R, Alessandrini S, Iwanejko G, Domenici R, Mei E, Miano A, Marani M, Bottaro G, Spina M, Dotti M, Montanelli A, Barbato M, Viola F, Lazzari R, Vallini M, Guariso G, Plebani M, Cataldo F, Traverso G, Ventura A et al (1996) The coeliac iceberg in Italy. A multicentre antigliadin antibodies screening for coeliac disease in school-age subjects. Acta Paediatr Suppl 412:29–35Google Scholar
  7. 7.
    Kolho KL, Färkkilä MA, Savilahti E (1998) Undiagnosed coeliac disease is common in Finnish adults. Scand J Gastroenterol 33:1280–1283Google Scholar
  8. 8.
    van Heel DA, West J (2006) Recent advances in coeliac disease. Gut 55(7):1037–1046Google Scholar
  9. 9.
    Freeman HJ (2008) Adult celiac disease in the elderly. World J Gastroenterol 14(45):6911–6914Google Scholar
  10. 10.
    Leeds JS, Hopper AD, Sanders DS (2008) Coeliac disease. Br Med Bull 88(1):157–170Google Scholar
  11. 11.
    Plot L, Amital H (2009) Infectious associations of celiac disease. Autoimmun Rev 8(4):316–319Google Scholar
  12. 12.
    Torres MI, López Casado MA, Ríos A (2007) New aspects in celiac disease. World J Gastroenterol 13(8):1156–1161Google Scholar
  13. 13.
    AGA (2001) American Gastroenterological Association medical position statement: celiac sprue. Gastroenterology 120(6):1522–1525Google Scholar
  14. 14.
    Ciclitira PJ, King AL, Fraser JS (2001) AGA technical review on celiac sprue. American Gastroenterological Association. Gastroenterology 120(6):1526–1540Google Scholar
  15. 15.
    Fasano A, Catassi C (2001) Current approaches to diagnosis and treatment of celiac disease: an evolving sprectrum. Gastroenterology 120(3):636–651Google Scholar
  16. 16.
    Farrell RJ, Kelly CP N (2002) Celiac sprue. N Engl J Med 346(3):180–188Google Scholar
  17. 17.
    Green PHR, Stavropoulos SN, Panagi SG, Goldstein SL, Mcmahon DJ, Absan H, Neugut AI (2001) Characteristics of adult celiac disease in the USA: results of a national survey. Am J Gastroenterol 96(1):126–131Google Scholar
  18. 18.
    Hill PG, McMillan SA (2006) Anti-tissue transglutaminase antibodies and their role in the investigation of coeliac disease. Ann Clin Biochem 43(Pt2):105–117Google Scholar
  19. 19.
    Bottaro G, Cataldo F, Rotolo N, Spina M, Corazza GR (1999) The clinical pattern of subclinical/silent celiac disease: an analysis on 1026 consecutive cases. Am J Gastroenterol 94(3):691–696Google Scholar
  20. 20.
    Mäki M, Holm K, Koskimies S, Hällström O, Visakorpi JK (1990) Normal small bowel biopsy followed by coeliac disease. Arch Dis Child 65(10):1137–1141Google Scholar
  21. 21.
    Collin P, Kaukinen K, Mäki M (1999) Clinical features of celiac disease today. Dig Dis 17(2):100–106Google Scholar
  22. 22.
    Holmes GK (2001) Potential and latent coeliac disease. Eur J Gastroenterol Hepatol 13(9):1057–1060Google Scholar
  23. 23.
    Mäki M, Holm K, Collin P, Savilahti E (1991) Increase in gamma/delta T cell receptor bearing lymphocytes in normal small bowel mucosa in latent coeliac disease. Gut 32(11):1412–1414Google Scholar
  24. 24.
    Kim CY, Quarsten H, Bergseng E, Khosla C, Sollid LM (2004) Structural basis for HLA-DQ2-mediated presentation of gluten epitopes in celiac disease. Proc Natl Acad Sci USA 101(12):4175–4179Google Scholar
  25. 25.
    Louka AS, Sollid LM (2003) HLA in coeliac disease: unravelling the complex genetics of a complex disorder. Tissue Antigens 61(2):105–117Google Scholar
  26. 26.
    Schuppan D, Dennis MD, Kelly CP (2005) Celiac disease: epidemiology, pathogenesis, diagnosis, and nutritional management. Nutr Clin Care 8(2):54–69Google Scholar
  27. 27.
    van Heel DA, Franke L, Hunt KA, Gwilliam R, Zhernakova A, Inouye M, Wapenaar MC, Barnardo MC, Bethel G, Holmes GK, Feighery C, Jewell D, Kelleher D, Kumar P, Travis S, Walters JR, Sanders DS, Howdle P, Swift J, Playford RJ, McLaren WM, Mearin ML, Mulder CJ, McManus R, McGinnis R, Cardon LR, Deloukas P, Wijmenga C (2007) A genome-wide association study for celiac disease identifies risk variants in the region harboring IL2 and IL21. Nat Genet 39(7):827–829Google Scholar
  28. 28.
    McGough N, Cummings JH (2005) Coeliac disease: a diverse clinical syndrome caused by intolerance of wheat, barley and rye. Proc Nutr Soc 64(4):434–450Google Scholar
  29. 29.
    Green PH, Cellier C (2007) Celiac disease. N Engl J Med 357(17):1731–1743Google Scholar
  30. 30.
    Molberg Ø, Solheim FN, Jensen T, Lundin KE, Arentz-Hansen H, Anderson OD, Kjersti UA, Sollid LM (2003) Intestinal T-cell responses to high-molecular-weight glutenins in celiac disease. Gastroenterology 125(2):337–344Google Scholar
  31. 31.
    Koning F, Gilissen L, Wijmenga C (2005) Gluten: a two-edged sword. Immunopathogenesis of celiac disease. Springer Semin Immunopathol 27(2):217–232Google Scholar
  32. 32.
    van de Kamer JH, Weijers HA, Dicke WK (1953) Coeliac disease. IV. An investigation into the injurious constituents of wheat in connection with their action on patients with coeliac disease. Acta Paediatr 42(3):223–231Google Scholar
  33. 33.
    Vader LW, De Ru A, van der Wal Y, Kooy YM, Benckhuijsen W, Mearin ML, Drijfhout JW, van Veelen P, Koning F (2002) Specificity of tissue transglutaminase explains cereal toxicity in celiac disease. J Exp Med 195(5):643–649Google Scholar
  34. 34.
    Stepniak D, Koning F (2006) Celiac disease—sandwiched between innate and adaptive immunity. Hum Immunol 67(6):460–468Google Scholar
  35. 35.
    Skovbjerg H, Hansen GH, Niels-Christiansen LL, Anthonsen D, Ascher H, Midhagen G, Hallert C, Norén O, Sjöström H (2004) Intestinal tissue transglutaminase in coeliac disease of children and adults: ultrastructural localization and variation in expression. Scand J Gastroenterol 39(12):1219–1227Google Scholar
  36. 36.
    Lundin KE, Scott H, Hansen T, Paulsen G, Halstensen TS, Fausa O, Thorsby E, Sollid LM (1993) Gliadin-specific, HLA-DQ(alpha 1*0501, beta 1*0201) restricted T cells isolated from the small intestinal mucosa of celiac disease patients. J Exp Med 178(1):187–196Google Scholar
  37. 37.
    Mowat AM (2003) Coeliac disease—a meeting point for genetics, immunology, and protein chemistry. Lancet 361(9365):1290–1292Google Scholar
  38. 38.
    Shan L, Molberg Ø, Parrot I, Hausch F, Filiz F, Gray GM, Sollid LM, Khosla C (2002) Structural basis for gluten intolerance in celiac sprue. Science 297(5590):2275–2279Google Scholar
  39. 39.
    Revised criteria for diagnosis of celiac disease (1990) Report of working group of European Society of Paediatric Gastroenterology and Nutrition. Arch Dis Child 65(8):909–911Google Scholar
  40. 40.
    Goldstein NS (2004) Non-gluten sensitivity-related small bowel villous flattening with increased intraepithelial lymphocytes: not all that flattens is celiac sprue. Am J Clin Pathol 121(4):546–550Google Scholar
  41. 41.
    Kaukinen K, Mäki M, Partanen J, Sievänen H, Collin P (2001) Celiac disease without villous atrophy: revision of criteria called for. Dig Dis Sci 46(4):879–887Google Scholar
  42. 42.
    Paparo F, Petrone E, Tosco A, Maglio M, Borrelli M, Salvati VM, Miele E, Greco L, Auricchio S, Troncone R (2005) Clinical, HLA, and small bowel immunohistochemical features of children with positive serum antiendomysium antibodies and architecturally normal small intestinal mucosa. Am J Gastroenterol 100(10):2294–2298Google Scholar
  43. 43.
    Hill ID, Dirks MH, Liptak GS, Colletti RB, Fasano A, Guandalini S, Hoffenberg EJ, Horvath K, Murray JA, Pivor M, Seidman EG, Society NA, North American Society for Pediatric Gastroenterology, Hepatology and Nutrition (2005) Guideline for the diagnosis and treatment of celiac disease in children: recommendations of the North American Society for Pediatric Gastroenterology, Hepatology and Nutrition. J Pediatr Gastroenterol Nutr 40(1):1–19Google Scholar
  44. 44.
    Collin P, Mäki M, Kaukinen K (2004) Complete small intestine mucosal recovery is obtainable in the treatment of celiac disease. Gastrointest Endosc 59(1):158–159Google Scholar
  45. 45.
    Kaukinen K, Collin P, Laurila K, Kaartinen T, Partanen MM (2007) Resurrection of gliadin antibodies in coeliac disease. Deamidated gliadin peptide antibody test provides additional diagnostic benefit. Scand J Gastroenterol 42(12):1428–1433Google Scholar
  46. 46.
    Troncone R, Ferguson A (1991) Anti-gliadin antibodies. J Pediatr Gastroenterol Nutr 12(2):150–158CrossRefGoogle Scholar
  47. 47.
    Dahlbom I, Olsson M, Forooz NK, Sjöholm AG, Truedsson L, Hansson T (2005) Immunoglobulin G (IgG) anti-tissue transglutaminase antibodies used as markers for IgA-deficient celiac disease patients. Clin Diagn Lab Immunol 12(2):254–258Google Scholar
  48. 48.
    Cataldo F, Marino V, Bottaro G, Greco P, Ventura A (1997) Celiac disease and selective immunoglobulin A deficiency. J Pediatric 131(2):306–308Google Scholar
  49. 49.
    Chorzelski TP, Beutner EH, Sulej J, Tchorzewska H, Jablonska S, Kumar V, Kapuscinska A (1984) IgA anti-endomysium antibody. A new immunological marker of dermatitis herpetiformis and coeliac disease. Br J Dermatol 111(4):395–402Google Scholar
  50. 50.
    Kárpáti S, Bürgin-Wolff A, Krieg T, Meurer M, Stolz W, Braun-Falco O (1990) Binding to human jejunum of serum IgA antibody from children with celiac disease. Lancet 336(8727):1335–1338Google Scholar
  51. 51.
    Ladinser B, Rossipal E, Pittschieler K (1994) Endomysium antibodies in coeliac disease: an improved method. Gut 35(6):776–778Google Scholar
  52. 52.
    Volta U, Molinaro N, De Franceschi L, Fratangelo D, Bianchi FB (1995) IgA anti-endomysial antibodies on human unbilical cord tissue for celiac disease screening. Dig Dis Sci 40(9):1902–1905Google Scholar
  53. 53.
    Lorand L, Graham RM (2003) Transglutaminases: crosslinking enzymes with pleiotropic functions. Nat Rev Mol Cell Biol 4(2):140–156Google Scholar
  54. 54.
    Sollid LM, Molberg Ø, McAdam S, Lundin KE (1997) Autoantibodies in coeliac disease: tissue transglutaminase—guilt by association? Gut 41(6):851–852CrossRefGoogle Scholar
  55. 55.
    Dieterich W, Ehnis T, Bauer M, Donner P, Volta U, Riecken EO, Schuppan D (1997) Identification of tissue transglutaminase as the autoantigen of celiac disease. Nat Med 3(7):797–801Google Scholar
  56. 56.
    Alaedini A, Green PH (2005) Narrative review: celiac disease: understanding a complex autoimmune disorder. Ann Intern Med 142(4):289–298Google Scholar
  57. 57.
    Rostom A, Murray JA, Kagnoff MF (2006) American Gastroenterological Association (AGA) Institute technical review on the diagnosis and management of celiac disease. Gastroenterology 131(6):1981–2002Google Scholar
  58. 58.
    Schuppan D (2000) Current concepts of celiac disease pathogenesis. Gastroenterology 119(1):234–242Google Scholar
  59. 59.
    Sulkanen S, Halttunen T, Laurila K, Kolho KL, Korponay-Szabó IR, Sarnesto A, Savilahti E, Collin P, Mäki M (1998) Tissue transglutaminase autoantibody enzyme-linked immunosorbent assay in detecting celiac disease. Gastroenterology 115(6):1322–1328Google Scholar
  60. 60.
    Fasano A (1999) Tissue transglutaminase: the holy grail for the diagnosis of celiac disease, at last? J Pediatr 134(2):134–135Google Scholar
  61. 61.
    Sárdy M, Odenthal U, Kárpáti S, Paulsson M, Smyth N (1999) Recombinant human tissue transglutaminase ELISA for the diagnosis of gluten-sensitive enteropathy. Clin Chem 45(12):2142–2149Google Scholar
  62. 62.
    Troncone R, Maurano F, Rossi M, Micillo M, Greco L, Auricchio R, Salerno G, Salvatore F, Sacchetti L (1999) IgA antibodies to tissue transglutaminase: an effective diagnostic test for celiac disease. J Pediatr 134(2):166–171Google Scholar
  63. 63.
    Sblattero D, Berti I, Trevisiol C, Marzari R, Tommasini A, Bradbury A, Fasano A, Ventura A, Not T (2000) Human recombinant tissue transglutaminase ELISA: an innovative diagnostic assay for celiac disease. Am J Gastroenterol 95(5):1253–1257Google Scholar
  64. 64.
    Sugai E, Selvaggio G, Vazquez H, Viola M, Mazure R, Pizarro B, Smecuol E, Flores D, Pedreira S, Mauriño E, Gomez JC, Bai JC (2000) Tissue transglutaminase antibodies in celiac disease: assessment of a commercial kit. Am J Gastroenterol 95(9):2318–2322Google Scholar
  65. 65.
    Fabiani E, Catassi C, International working group (2001) The serum IgA class anti-tissue transglutaminase antibodies in the diagnosis and follow up of coeliac disease. Results of an international multi-centre study. International Working Group on Eu-tTG. Eur J Gastroenterol Hepatol 13(6):659–665Google Scholar
  66. 66.
    Carroccio A, Vitale G, Di Prima L, Chifari N, Napoli S, La Russa C, Gulotta G, Averna MR, Montalto G, Mansueto S, Notarbartolo A (2002) Comparison of anti-transglutaminase ELISAs and an anti-endomysial antibody assay in the diagnosis of celiac disease: a prospective study. Clin Chem 48(9):1546–1550Google Scholar
  67. 67.
    Hansson T, Dahlbom I, Rogberg S, Dannaeus A, Hopfl P, Gut H, Kraaz W, Klareskog L (2002) Recombinant human tissue transglutaminase for diagnosis and follow-up of childhood coeliac disease. Pediatr Res 51(6):700–705Google Scholar
  68. 68.
    Martini S, Mengozzi G, Aimo G, Giorda L, Pagni R, Guidetti CS (2002) Comparative evaluation of serologic tests for celiac disease diagnosis and follow-up. Clin Chem 48(6 Pt 1):960–963Google Scholar
  69. 69.
    Wolters V, Vooijs-Moulaert AF, Burger H, Brooimans R, De Schryver J, Rijkers G, Houwen R (2002) Human tissue transglutaminase enzyme linked immunosorbent assay outperforms both the guinea pig based tissue transglutaminase assay and anti-endomysium antibodies when screening for coeliac disease. Eur J Pediatr 161(5):284–287Google Scholar
  70. 70.
    Johnston SD, McMillan SA, Collins JS, Tham TC, McDougall NI, Murphy P (2003) A comparison of antibodies to tissue transglutaminase with conventional serological tests in the diagnosis of coeliac disease. Eur J Gastroenterol Hepatol 15(9):1001–1004Google Scholar
  71. 71.
    Korponay-Szabó IR, Dahlbom I, Laurila K, Koskinen S, Woolley N, Partanen J, Kovács JB, Mäki M, Hansson T (2003) Elevation of IgG antibodies against tissue transglutaminase as a diagnostic tool for coeliac disease in selective IgA deficiency. Gut 52(11):1567–1571Google Scholar
  72. 72.
    Tesei N, Sugai E, Vázquez H, Smecuol E, Niveloni S, Mazure R, Moreno ML, Gomez JC, Mauriño E, Bai JC (2003) Antibodies to human recombinant tissue transglutaminase may detect coeliac disease patients undiagnosed by endomysial antibodies. Aliment Pharmacol Ther 17(11):1415–1423Google Scholar
  73. 73.
    Hill PG, Forsyth JM, Semeraro D, Holmes GKT (2004) IgA antibodies to human tissue transglutaminase: audit of routine practice confirms high diagnostic accuracy. Scand J Gastroenterol 39(11):1078–1082Google Scholar
  74. 74.
    Lampasona V, Bazzigaluppi E, Barera G, Bonifacio E (1998) Tissue transglutaminase and combined screening for coeliac disease and type 1 diabetes-associated autoantibodies. Lancet 352(9135):1192–1193Google Scholar
  75. 75.
    Blackweel PJ, Hill PG, Holmes GKT (2002) Autoantibodies to human tissue transglutaminase: superior predictors of coeliac disease. Scand J Gastroenterol 37(11):1282–1285Google Scholar
  76. 76.
    Perticarari S, Znidarcic C, Granzotto M, Parco S (2003) Human recombinant tissue-transglutaminase antibodies: evaluation of a commercial kit and comparison of methods using guinea pig antigen. Minerva Pediatr 55(3):277–282Google Scholar
  77. 77.
    Wong RCW, Wilson RJ, Steele RH, Radford-Smith G, Adelstein S (2002) A comparison of 13 guinea pig and human anti-tissue transglutaminase antibody ELISA kits. J Clin Pathol 55(7):488–494Google Scholar
  78. 78.
    Villalta D, Crovato M, Stella S, Tonutti E, Tozzoli R, Bizzaro N (2005) False positive reactions for IgA and IgG anti-tissue transglutaminase antibodies in liver cirrhosis are common and method-dependent. Clin Chim Acta 356(1–2):102–109Google Scholar
  79. 79.
    Van Meensel B, Hiele M, Hoffman I, Vermeire S, Rutgeerts P, Geboes K, Bossuyt X (2004) Diagnostic accuracy of ten second-generation (human) tissue transglutaminase antibody assays in celiac disease. Clin Chem 50(11):2125–2135Google Scholar
  80. 80.
    NIH (2004) National Institutes of Health consensus development conference statement on celiac disease, 28–30 June, 2004. Gastroenterology 128(4 Suppl 1):S1–9Google Scholar
  81. 81.
    Wieser H (2007) Chemistry of gluten proteins. Food Microb 24(2):115–119Google Scholar
  82. 82.
    Morón B, Bethune MT, Comino I, Manyani H, Ferragud M, López MC, Cebolla A, Khosla C, Sousa C (2008) Toward the assessment of food toxicity for celiac patients: characterization of monoclonal antibodies to a main immunogenic gluten peptide. PLoS ONE 3(5):e2294Google Scholar
  83. 83.
    Khosla C, Gray GM, Sollid LM (2005) Putative efficacy and dosage of prolyl endopeptidase for digesting and detoxifying gliadin peptides. Gastroenterology 129(4):1362–1363Google Scholar
  84. 84.
    Codex Alimentarius (2003) Draft revised standards for gluten-free foods, report of 25th session of the Codex Committee on Nutrition and Foods for Special Dietary Uses, November 2003Google Scholar
  85. 85.
    Hischenhuber C, Crevel R, Jarry B, Mäki M, Moneret-Vautrin DA, Romano A, Troncone R, Ward R (2006) Review article: safe amounts of gluten for patients with wheat allergy or coeliac disease. Aliment Pharmacol Ther 23(5):559–575Google Scholar
  86. 86.
    Bermudo Redondo MC, Griffin PB, Garzon Ransanz M, Ellis HJ, Ciclitira PJ, O'Sullivan CK (2005) Monoclonal antibody-based competitive assay for the sensitive detection of coeliac disease toxic prolamins. Anal Chim Acta 551(1–2):105–114Google Scholar
  87. 87.
    Denery-Papini S, Samson MF, Autran JC (2000) Antipeptide antibodies directed against omega-gliadins for the detection of sequences from bread and durum wheats. Food Agric Immunol 12(1):67–75Google Scholar
  88. 88.
    Nicolas Y, Denery-Papini S, Martinant JP, Popineau Y (2000) Suitability of a competitive ELISA using anti-peptide antibodies for determination of the gliadin content of wheat flour: comparison with biochemical methods. Food Agric Immunol 12(1):53–65Google Scholar
  89. 89.
    Horwitz W (2000) Official method 991.19. Official methods of analysis of AOAC International, 17th edn. AOAC, Gaithersburg, p 15Google Scholar
  90. 90.
    Meyer J, Karst U (2001) Enzyme-linked immunosorbent assays based on peroxidase labels and enzyme-amplified lanthanide luminescence detection. Analyst 126(2):175–178Google Scholar
  91. 91.
    Rumbo M, Chirdo FG, Fossati CA, Añón MC (2001) Analysis of the effects of heat treatment on gliadin immunochemical quantification using a panel of anti-prolamin antibodies. J Agric Food Chem 49(12):5719–5726Google Scholar
  92. 92.
    Prabhasankar P, Sai-Manohar R (2002) Development of enzyme-linked immunosorbent assay for evaluation of chapati-making quality of wheat varieties. J Agric Food Chem 50(25):7455–7460Google Scholar
  93. 93.
    Allmann M, Candrian U, Hofelein C, Luthy J (1993) Polymerase chain-reaction (PCR)—a possible alternative to immunochemical methods assuring safety and quality of food - detection of wheat contamination in non-wheat food-products. Z Lebensm Unters Forsch 196(3):248–251Google Scholar
  94. 94.
    Köppel E, Stadler M, Luthy J, Hubner P (1998) Detection of wheat contamination in oats by polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). Z Lebensm Unters Forsch 206(6):399–403Google Scholar
  95. 95.
    Dahinden I, von Büren M, Lüthy J (2001) A quantitative competitive PCR system to detect contamination of wheat, barley or rye in gluten-free food for coeliac patients. Eur Food Res Technol 212(2):228–233Google Scholar
  96. 96.
    Olexová L, Dovicovicová L, Svec M, Siekel P, Kuchta T (2006) Detection of gluten-containing cereals in flours and “gluten-free” bakery products by polymerase chain reaction. Food Control 17(3):234–237Google Scholar
  97. 97.
    Piknova L, Brezna B, Kuchta T (2008) Detection of gluten-containing cereals in food by 5′-nuclease real-time polymerase chain reaction. J Food Nutr Res 47(3):114–119Google Scholar
  98. 98.
    Henterich N, Osman AA, Méndez E, Mothes T (2003) Assay of gliadin by real-time immunopolymerase chain reaction. Nahrung 47(5):345–348Google Scholar
  99. 99.
    Méndez E, Valdés I, Camafeita E (2000) Methods in molecular biology. Chapman, New JerseyGoogle Scholar
  100. 100.
    Méndez E, Camafeita E, SanSebastian J, Valle I, Solis J, MayerPosner FJ, Suckau D, Marfisi C, Soriano F (1995) Direct identification of wheat gliadins and related cereal prolamins by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. J Mass Spectrom S123-S128Google Scholar
  101. 101.
    Camafeita E, Solis J, Alfonso P, Lopez JA, Sorell L, Mendez E (1993) Selective identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of different types of gluten in foods made with cereal mixtures. Z Lebensm Unters Forsch 196(3):248–251Google Scholar
  102. 102.
    Hernando A, Valdes I, Méndez H (2003) New strategy for the determination of gliadins in maize- or rice-based foods matrix-assisted laser desorption/ionization time-of-flight mass spectrometry: fractionation of gliadins from maize or rice prolamins by acidic treatment. J Mass Spectrom 38(8):862–871Google Scholar
  103. 103.
    Nicolas Y, Martinant JP, Denery-Papini S, Popineau Y (1998) Analysis of wheat storage proteins by exhaustive sequential extraction followed by RP-HPLC and nitrogen determination. J Sci Food Agric 77(1):96–102Google Scholar
  104. 104.
    Wieser H, Seilmeier W, Belitz HD (1994) Quantitative-determination of gliadin subgroups from different wheat cultivars. J Cereal Sci 19(2):149–155Google Scholar
  105. 105.
    Capparelli R, Ventimiglia I, Longobardo L, Iannelli D (2005) Quantification of gliadin levels to the picogram level by flow cytometry. Cytometry A 63A(2):108–113Google Scholar
  106. 106.
    Comas-Riu J, Rius N (2009) Flow cytometry applications in the food industry. J Ind Microbiol Biotech 36(8):999–1011Google Scholar
  107. 107.
    Skerritt J, Hill A (1991) Enzyme immunoassay for determination of gluten in foods: collaborative study. J Assoc Anal Chem 74(2):257–264Google Scholar
  108. 108.
    Wieser H, Seilmeier W, Belitz H-D (1994) Quantitative determination of gliadin subgroups from different wheat cultivars. J Cereal Sci 19(3):149–155Google Scholar
  109. 109.
    Thompson T, Méndez E (2008) Commercial assays to assess gluten content of gluten-free foods: why they are not created equal. J Am Diet Assoc 108(10):1682–1687Google Scholar
  110. 110.
    Valdés I, Garcia E, Llorente M, Méndez E (2003) Innovative approach to low-level gluten determination in foods using a novel sandwich enzyme-linked immunosorbent assay protocol. Eur J Gastroenterol Hepatol 15(5):465–474Google Scholar
  111. 111.
    Codex Alimentarius Commission (2005) Status of endorsement of methods of analysis and sampling. Codex Alimentarius ALINORM 05/28/23. Appendix III, Rome, pp 31–40Google Scholar
  112. 112.
    Codex Alimentarius Commission (2004) Draft revised strandard for gluten-free foods. Codex Alimentarius ALINORM 04/27/26. Appendix III, Rome, pp 42-43Google Scholar
  113. 113.
    Kanerva PM, Sontag-Strohm TS (2006) Problems in detecting prolamins contaminants in oat-based foods by commercial ELISA kits. 9th international gluten workshop, September 2006; p 33. Available via American Association of Cereal Chemists. www.aaccnet.org/meetings/glutenworkshop/pdfs/programbook.pdf. Accessed 2 Nov 2009
  114. 114.
    De Stefano L, Rossi M, Staiano M, Mamone G, Parracino A, Rotiroti L, Rendina I, Rossi M, D’Auria S (2006) Glutamine-binding protein from escherichia coli specifically binds a wheat gliadin peptide allowing the design of a new porous silicon-based optical biosensor. J Proteome Res 5:1241–1245Google Scholar
  115. 115.
    Corres JM, Matias IR, Bravo J, Arregui FJ (2008) Tapered optical fiber biosensor for the detection of anti-gliadin antibodies. Sens Actuators B Chem 135:166–171Google Scholar
  116. 116.
    Rodríguez-Moraz S, López de Alda MJ, Barceló D (2006) Biosensors as useful tools for environmental analysis and monitoring. Anal Bioanal Chem 386(4):1025–1041Google Scholar
  117. 117.
    Nassef HM, Bermudo Redondo MC, Ciclitira PJ, Ellis HJ, Fragoso A, O’Sullivan CK (2008) Electrochemical immunosensor for detection of celiac disease toxic gliadin in foodstuff. Anal Chem 80(23):9265–9271Google Scholar
  118. 118.
    Nassef HM, Civit L, Fragoso A, O’Sullivan CK (2009) Amperometric immunosensor for detection of celiac disease toxic gliadin based on fab fragments. Anal Chem 81(13):5299–5307Google Scholar
  119. 119.
    Balkenhohl T, Lisdat F (2007) An impedimetric immunosensors for the detection of autoantibodies directed against gliadins. Analyst 132(4):314–322Google Scholar
  120. 120.
    Balkenhohl T, Lisdat F (2007) Screen-printed electrodes as impedimetric immunosensors for the detection of anti-transglutaminase antibodies in human sera. Anal Chim Acta 597(1):50–57Google Scholar
  121. 121.
    Pividori MI, Lermo A, Bonanni A, Alegret S, del Valle M (2009) Electrochemical immunosensor for the diagnosis of celiac disease. Anal Biochem 388(2):229–234Google Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Marta Maria Pereira da Silva Neves
    • 1
    • 2
    • 3
  • Maria Begoña González-Garcia
    • 3
  • Hendrikus Petrus Antonius Nouws
    • 2
  • Cristina Delerue-Matos
    • 2
  • Alice Santos-Silva
    • 1
    • 4
  • Agustín Costa-García
    • 3
  1. 1.Faculdade de Farmácia da Universidade do PortoPortoPortugal
  2. 2.REQUIMTEInstituto Superior de Engenharia do PortoPortoPortugal
  3. 3.Departamento de Química Física y AnalíticaUniversidad de OviedoOviedoSpain
  4. 4.Instituto de Biologia Molecular e Celular da Universidade do PortoPortoPortugal

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