Abstract
The characterization of mass-transfer processes in a chromatographic column during a separation process is essential, since the influence of the mass-transfer kinetics on the shape of the chromatographic band profiles and on the efficiency of the separation is crucial. Several sources of mass transfer in a chromatographic bed have been identified and studied: the axial dispersion in the stream of mobile phase, the external mass-transfer resistance, intraparticle diffusion, and the kinetics of adsorption–desorption. We measured and compared the characteristics and performance of a new brand of shell particles and those of a conventional brand of totally porous silica particles. The shell stationary phase was made of 2.7-µm superficially porous particles (a 1.7-µm solid core is covered with a 0.5-µm-thick shell of porous silica). The other material consisted of totally porous particles of conventional 3.5-µm commercial silica. We measured the first and second central moments of the peaks of human insulin over a wide range of mobile phase velocities (from 0.02 to 1.3 mL/min) at 20°C. The plate height equations were constructed and the axial dispersion, external mass transfer, as well as the intraparticle diffusion coefficients were calculated for the two stationary phases.
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Acknowledgements
This work was supported by grants GVOP-3.2.1-0168, RET 008/2005, OTKA 75717, and OTKA-NKTH 68863.
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Kiss, I., Bacskay, I., Kilár, F. et al. Comparison of the mass transfer in totally porous and superficially porous stationary phases in liquid chromatography. Anal Bioanal Chem 397, 1307–1314 (2010). https://doi.org/10.1007/s00216-010-3627-6
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DOI: https://doi.org/10.1007/s00216-010-3627-6