Determination of aflatoxin B1 in alcoholic beverages: comparison of one- and two-photon-induced fluorescence
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The qualitative and quantitative analysis of aflatoxin B1 in a model system (water/ethanol), in different wines and in beer using one- and two-photon-induced fluorescence is discussed. The absorption and fluorescence properties of aflatoxin B1 depend on the solvent and pH. The two-photon-absorption cross-section was calculated for aflatoxin B1 in beer and wine (σ2 ∼ 25 GM) for excitation at 720 nm. A comparison of the one- and two-photon- induced fluorescence results showed that the disturbance due to background emission originating from matrix constituents is significantly reduced under two-photon-excitation conditions. The limit of detection for the one- and two-photon-induced fluorescence was determined.
KeywordsAflatoxin B1 Fluorescence Two-photon absorption Two-photon-absorption cross-section Beer Wine
The authors express their thanks to the German Federal Ministry of Education and Research (BMBF) and the project executing organisation Jülich (PtJ) for financial support within the joint research project ProSenso.net2 (PSn2).
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