Analytical and Bioanalytical Chemistry

, Volume 397, Issue 1, pp 381–388 | Cite as

CMOS arrays as chemiluminescence detectors on microfluidic devices

  • Eunice R. G. O. Rodrigues
  • Rui A. S. Lapa
Original Paper


A simple, low-cost process to integrate complementary metal oxide semiconductor array detectors (CMOSAD) for chemiluminescence is presented, evaluated, and applied to the determination of nitrite in ground water samples. CMOS arrays of different brands (obtained from commercial image sensors) were adapted as chemiluminescence detectors on microfluidic devices. The performance of the CMOSADs was evaluated in the visible zone of the spectrum using a tungsten halogen lamp as light source. Intrinsic parameters assessed included signal stability, spectral response, dark current, and signal-to-noise ratio. Thereafter, the CMOSADs were integrated on microfluidic devices and their performances in quantitative analysis were assessed with the chemiluminometric reaction of hydrogen peroxide with luminol, catalyzed with hexacyanoferrate (III). The parameters assessed were sensitivity, linear range, detection limit, reproducibility, correlation coefficient of the calibration curves, and baseline drift during measurements. The CMOSAD with the best performance was selected to assess the applicability of the developed microfluidic devices with the integrated detector. The microfluidic system permitted the determination of nitrite with both good precision and good recovery values in the analysis of ground water samples. Integration was easily achieved and enabled the development of a simple, low-cost, and feasible alternative to conventional detectors.


Microfluidic device CMOS array Chemiluminescence detector PMMA Nitrite determination Multi-pumping 



E. R. G. O. Rodrigues acknowledges FCT for the PhD grant (SFRH/BD/23417/2005).


  1. 1.
    Greenway GM, Nelstrop LJ, Port SN (2000) Anal Chim Acta 405:43–50CrossRefGoogle Scholar
  2. 2.
    Schwarz MA, Hauser PC (2001) Lab Chip 1:1–6CrossRefGoogle Scholar
  3. 3.
    Tyrrell E, Gibson C, MacCraith BD, Gray D, Byrne P, Kent N, Burke C, Paull B (2004) Lab Chip 4:384–390CrossRefGoogle Scholar
  4. 4.
    Jorgensen AM, Mogensen KB, Kutter JP, Geschke O (2003) Sens Actuators B 90:15–21CrossRefGoogle Scholar
  5. 5.
    Carretero AS, Fernandez JR, Bowie AR, Worsfold PJ (2000) Analyst 125:387–390CrossRefGoogle Scholar
  6. 6.
    Lu U, Hu BC-P, Shih Y-C, Wu C-Y, Yang Y-S (2004) Biosens Bioelectron 19:1185–1191CrossRefGoogle Scholar
  7. 7.
    Magnan P (2003) Nucl Instr Methods Phys Res A 504:199–212CrossRefGoogle Scholar
  8. 8.
    Litwiller D (2005) Photonics spectra. Accessed 09 Out 2009
  9. 9.
    Rodrigues ERGO, Lapa RAS (2009) Anal Sci 25:443–448CrossRefGoogle Scholar
  10. 10.
    Mikuška P, Večeřa Z, Zdráhal Z (1995) Anal Chim Acta 316:261–268CrossRefGoogle Scholar
  11. 11.
    Lu C, Qu F, Lin J-M, Yamada M (2002) Anal Chim Acta 474:107–114CrossRefGoogle Scholar
  12. 12.
    Piwoni MD (1998) In: Clesceri LS (ed) Standard methods for the examination of water and wastewater, 20th edn. American Public Health Association, Washington, DCGoogle Scholar
  13. 13.
    Santos WJR, Lima PR, Tanaka AA, Tanaka SMCN, Kubota LT (2009) Food Chem 113:1206–1211CrossRefGoogle Scholar
  14. 14.
    Moorcroft MJ, Davis J, Compton RG (2001) Talanta 54:785–803CrossRefGoogle Scholar
  15. 15.
    Hsu J, Arcot J, Lee NA (2009) Food Chem 115:334–339CrossRefGoogle Scholar
  16. 16.
    Ribeiro MFT, Dias ACB, Santos JLM, Fernandes E, Lima JLFC, Zagatto EAG (2007) J Biomol Screen 12:875–880CrossRefGoogle Scholar
  17. 17.
    Lima JLFC, Santos JLM, Dias ACB, Ribeiro MFT, Zagatto EAG (2004) Talanta 64:1091–1098CrossRefGoogle Scholar
  18. 18.
    Saha A, Goldstein S, Cabelli D, Czapski G (1998) Free Radic Biol Med 24:653–659CrossRefGoogle Scholar
  19. 19.
    Hayashi K, Sasaki S, Ikebukuro K, Karube I (1996) Anal Chim Acta 329:127–134CrossRefGoogle Scholar
  20. 20.
    Price D, Mantoura R, Worsfold P (1998) Anal Chim Acta 377:145–155CrossRefGoogle Scholar
  21. 21.
    Li J, Wang K-M, Yang X, Xiao D (1999) Anal Commun 36:195–197CrossRefGoogle Scholar
  22. 22.
    Bostick D, Hercules D (1975) Anal Chem 47:447–452CrossRefGoogle Scholar
  23. 23.
    Shaw F (1980) Analyst 105:11–17CrossRefGoogle Scholar
  24. 24.
    Borges EP, Fernandes EN, Rocha FRP, Reis BF (2002) Quim Nova 25:1191–1193CrossRefGoogle Scholar
  25. 25.
    Miller JC, Miller JN (2000) Statistics for analytical chemistry, 3rd edn. Horwood, New YorkGoogle Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Eunice R. G. O. Rodrigues
    • 1
  • Rui A. S. Lapa
    • 1
  1. 1.REQUIMTE, Serviço de Química-Física, Faculdade de Farmácia da Universidade do PortoPortoPortugal

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