Analytical and Bioanalytical Chemistry

, Volume 397, Issue 1, pp 233–241 | Cite as

Voltammetric detection of damage to DNA caused by nitro derivatives of fluorene using an electrochemical DNA biosensor

Original Paper


An electrochemical DNA biosensor based on the screen printed carbon paste electrode (SPCPE) with an immobilized layer of calf thymus double-stranded DNA has been used for in vitro investigation of the interaction between genotoxic nitro derivatives of fluorene (namely 2-nitrofluorene and 2,7-dinitrofluorene) and DNA. Two types of DNA damage have been detected at the DNA/SPCPE biosensor: first, that caused by direct association of the nitrofluorenes, for which an intercalation association has been found using the known DNA intercalators [Cu(phen)2]2+ and [Co(phen)3]3+ as competing agents, and, second, that caused by short-lived radicals generated by electrochemical reduction of the nitro group (observable under specific conditions only).


Anodic DPV response of DNA bases at the DNA/SPCPE (after baseline correction) recorded in AcB -methanol (99:1) medium; Eamp 50 mV, pulse width 100 ms, scan rate 20 mV s−1, Estep 5 mV, 36°C. DP voltammograms recorded at the DNA/SPCPE before (green curve) and after successive CV cathodic/anodic cycling between 0 and −1000 mV (15 scans; scan rate 50 mV s−1) in solution of 2-NF (c=1×10−5 mol L−1) in AcB -methanol (99:1) (red curve). Two different DNA/SPCPEs were used to record green and red curve


2-Nitrofluorene 2,7-Dinitrofluorene DNA biosensor Screen printed carbon paste electrode Electrochemical detection DNA damage 



This work was financially supported by the Ministry of Education, Youth and Sports of the Czech Republic (projects LC 06035, MSM 0021620857, and RP 14/63) and by the European Union Lifelong Learning Programme (Erasmus). J. L. thanks to the Grant Agency VEGA (project 1/0852/08) of the Ministry of Education of the Slovak Republic and Slovak Academy of Sciences for financial support.


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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Vlastimil Vyskočil
    • 1
  • Ján Labuda
    • 2
  • Jiří Barek
    • 1
  1. 1.Charles University in Prague, Faculty of Science, Department of Analytical ChemistryUNESCO Laboratory of Environmental ElectrochemistryPrague 2Czech Republic
  2. 2.Slovak University of Technology in Bratislava, Faculty of Chemical and Food Technology, Institute of Analytical ChemistryBratislavaSlovakia

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