Ultra-fast HPLC-ICP-MS analysis of oxaliplatin in patient urine
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A novel method for rapid HPLC-ICP-MS analysis of oxaliplatin in human urine was developed implementing a stationary HPLC phase with a particle size of 1.8 µm. The method allowed a cycle time of <1 min at a HPLC flow rate of 0.9 mL min−1. Procedural limits of detection of 0.05 µg L−1 oxaliplatin (150 fg on column) were obtained. Analysis of oxaliplatin in patient urine showed that accurate quantification of the intact drug demanded for storage at −80 °C and rapid measurement after thawing.
KeywordsOxaliplatin HPLC ICP-MS
Financial support was granted through the Austrian Science Fund (FWF-Project P16089-N03: “Speciation of cancerostatic Pt compounds in the environment”). Prof. Mader (Vienna University Hospital) is gratefully acknowledged for sampling of patient urine. Thomas Falta is acknowledged for performing the HILIC-ICP-MS measurements.
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