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Analytical and Bioanalytical Chemistry

, Volume 397, Issue 1, pp 401–406 | Cite as

Ultra-fast HPLC-ICP-MS analysis of oxaliplatin in patient urine

  • Gunda Koellensperger
  • Stephan Hann
Technical Note

Abstract

A novel method for rapid HPLC-ICP-MS analysis of oxaliplatin in human urine was developed implementing a stationary HPLC phase with a particle size of 1.8 µm. The method allowed a cycle time of <1 min at a HPLC flow rate of 0.9 mL min−1. Procedural limits of detection of 0.05 µg L−1 oxaliplatin (150 fg on column) were obtained. Analysis of oxaliplatin in patient urine showed that accurate quantification of the intact drug demanded for storage at −80 °C and rapid measurement after thawing.

Keywords

Oxaliplatin HPLC ICP-MS 

Notes

Acknowledgement

Financial support was granted through the Austrian Science Fund (FWF-Project P16089-N03: “Speciation of cancerostatic Pt compounds in the environment”). Prof. Mader (Vienna University Hospital) is gratefully acknowledged for sampling of patient urine. Thomas Falta is acknowledged for performing the HILIC-ICP-MS measurements.

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  1. 1.Department of Chemistry, Division of Analytical ChemistryUniversity of Natural Resources and Applied Life Sciences, BOKU, ViennaViennaAustria

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