Analytical and Bioanalytical Chemistry

, Volume 397, Issue 1, pp 173–179 | Cite as

Determination of adrenolytic drugs by SPME–LC–MS

  • Boguslaw Buszewski
  • Pawel Olszowy
  • Tomasz Ligor
  • Malgorzata Szultka
  • Jacek Nowaczyk
  • Maciej Jaworski
  • Marek Jackowski
Original Paper


Five adrenolytic drugs have been analyzed by liquid chromatography–mass spectrometry (LC–MS). Samples were prepared by solid-phase microextraction (SPME) using polypyrrole fibers coated on stainless steel support as an adsorbent for the drugs. Adsorption efficiencies were 95% and were close for all the drugs investigated. Relative standard deviations (RSD), calculated for samples prepared in standard solutions, were in the range 2.5–13%, however RSD values for the drugs in human plasma were 2.5–4.5%. Using LC–MS the limit of detection (LOD) and the limit of quantification (LOQ) were in the ranges 0.11–0.18 and 0.39–0.54 ng mL−1, respectively, for the five drugs.


Adrenolytic drugs Solid-phase microextraction High-performance liquid chromatography Mass spectrometry 



The author would like to thank Dr Sylwia Studzinska and Dr Monika Michel for their critical yet inspiring discussions during writing of this paper.

This work was supported by the Foundation for Polish Sciences (FNP) Professor’s Subsidy “Mistrz”, Ministry of Science and Higher Education (Warsaw, Poland) Grant. No. N204 268038 and by European Social Found, Polish National Budget, Kujawsko-pomorskie Voivodship Budget (within Sectoral Operational Programme Human Resources) “Krok w przyszlosc” and “Stypendia dla doktorantow 2008/2009-ZPORR”.

Then authors are grateful to Perlan Technologies (Poland) for supplying the Agilent Technologies Triple Quad LC–MS 6410.


  1. 1.
    Hoffman BB, Lefkowitz RJ (1996) Sympathomimetic drugs and adrenergic receptor antagonists. In Goodman and Gilman. The pharmacological basis of therapeutics, 9th ed. McGraw–Hill, New YorkGoogle Scholar
  2. 2.
    Chobanian AV, Bakris GL, Black HR (2003) Hypertens 42:1206–1252CrossRefGoogle Scholar
  3. 3.
    Chrysant SG (2007) Curr Hypertens Rev 3:21–28CrossRefGoogle Scholar
  4. 4.
    Derosa G, Salvadeo S, Cicero AFG (2006) Curr Clin Pharmacol 1:21–33CrossRefGoogle Scholar
  5. 5.
    Arias R, Jiménez RM, Alonso RM, Télez M, Arrieta I, Flores P, Ortiz-Lastra E (2001) J Chromatogr A 916:297–304CrossRefGoogle Scholar
  6. 6.
    Ahlquist RP (1948) Am J Physiol 153:586–600Google Scholar
  7. 7.
    Eldesoky ES, Derendorf H, Klotz U (2006) Curr Clin Pharmacol 1:35–46CrossRefGoogle Scholar
  8. 8.
    Leucuta SE, Vlase L (2006) Curr Clin Pharmacol 1:5–20CrossRefGoogle Scholar
  9. 9.
    Burnier M, Bullani R, Vogt B (2007) Curr Hypertens Rev 3:15–20CrossRefGoogle Scholar
  10. 10.
    Görög S (2007) Trends Anal Chem 26:12–17CrossRefGoogle Scholar
  11. 11.
    Ranta VP, Toropainen E, Talvitie A, Auriola S, Urtti A (2002) J Chromatogr B 772:81–87CrossRefGoogle Scholar
  12. 12.
    Needham SR, Brown PR, Duff K, Bell D (2000) J Chromatogr A 869:159–170CrossRefGoogle Scholar
  13. 13.
    Görög S (2006) Trends Anal Chem 25:755–757CrossRefGoogle Scholar
  14. 14.
    Urlich S (2000) J Chromatogr A 902:167–194CrossRefGoogle Scholar
  15. 15.
    Welerowicz T, Buszewski B (2005) Biomed Chromatogr 19:725–736CrossRefGoogle Scholar
  16. 16.
    Steiner F (2002) Angew Chem Int Edit 41:519–520CrossRefGoogle Scholar
  17. 17.
    Nerin C, Salafranca J, Aznar M, Batlle R (2009) Anal Bioanal Chem 393:809–833CrossRefGoogle Scholar
  18. 18.
    Risticevic S, Niri VH, Vuckovic D, Pawliszyn J (2009) Anal Bioanal Chem 393:781–795CrossRefGoogle Scholar
  19. 19.
    Giger W (2009) Anal Bioanal Chem 393:37–44CrossRefGoogle Scholar
  20. 20.
    Pawliszyn J (1997) Solid-phase microextraction: theory and practice. Wiley–VCH, New YorkGoogle Scholar
  21. 21.
    Schubert JK, Miekisch W, Fuchs P, Scherzer N, Lord H, Pawliszyn J, Mundkowski RG (2007) Clin Chim Acta 386:57–62CrossRefGoogle Scholar
  22. 22.
    Kataoka H (2005) Curr Pharm Anal 1:65–84CrossRefGoogle Scholar
  23. 23.
    Wu J, Lord HL, Pawliszyn J, Kataoka H (2000) J Microcolumn Sep 12:255–266CrossRefGoogle Scholar
  24. 24.
    Arthur CL, Pawliszyn J (1990) Anal Chem 62:2145–2148CrossRefGoogle Scholar
  25. 25.
    Zhang Z, Yang MJ, Pawliszyn J (1994) Anal Chem 66:844A–853ACrossRefGoogle Scholar
  26. 26.
    Kataoka H (2003) Trends Anal Chem 22:232–244CrossRefGoogle Scholar
  27. 27.
    Heberger K (2007) J Chromatogr A 1158:273–305CrossRefGoogle Scholar
  28. 28.
    Belpaire FM, Bogaert MG, Rosseneu M (1982) Eur J Clin Pharmacol 22:253–256CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Boguslaw Buszewski
    • 1
  • Pawel Olszowy
    • 1
  • Tomasz Ligor
    • 1
  • Malgorzata Szultka
    • 1
  • Jacek Nowaczyk
    • 2
  • Maciej Jaworski
    • 3
  • Marek Jackowski
    • 3
  1. 1.Department of Environmental Chemistry and Bioanalytics, Faculty of ChemistryNicolaus Copernicus UniversityTorunPoland
  2. 2.Department of Physical Chemistry and Physicochemistry of Polymers, Faculty of ChemistryNicolaus Copernicus UniversityTorunPoland
  3. 3.Department of Surgery, Collegium MedicumNicolaus Copernicus UniversityBydgoszczPoland

Personalised recommendations