Determination of adrenolytic drugs by SPME–LC–MS
- 257 Downloads
Five adrenolytic drugs have been analyzed by liquid chromatography–mass spectrometry (LC–MS). Samples were prepared by solid-phase microextraction (SPME) using polypyrrole fibers coated on stainless steel support as an adsorbent for the drugs. Adsorption efficiencies were 95% and were close for all the drugs investigated. Relative standard deviations (RSD), calculated for samples prepared in standard solutions, were in the range 2.5–13%, however RSD values for the drugs in human plasma were 2.5–4.5%. Using LC–MS the limit of detection (LOD) and the limit of quantification (LOQ) were in the ranges 0.11–0.18 and 0.39–0.54 ng mL−1, respectively, for the five drugs.
KeywordsAdrenolytic drugs Solid-phase microextraction High-performance liquid chromatography Mass spectrometry
The author would like to thank Dr Sylwia Studzinska and Dr Monika Michel for their critical yet inspiring discussions during writing of this paper.
This work was supported by the Foundation for Polish Sciences (FNP) Professor’s Subsidy “Mistrz”, Ministry of Science and Higher Education (Warsaw, Poland) Grant. No. N204 268038 and by European Social Found, Polish National Budget, Kujawsko-pomorskie Voivodship Budget (within Sectoral Operational Programme Human Resources) “Krok w przyszlosc” and “Stypendia dla doktorantow 2008/2009-ZPORR”.
Then authors are grateful to Perlan Technologies (Poland) for supplying the Agilent Technologies Triple Quad LC–MS 6410.
- 1.Hoffman BB, Lefkowitz RJ (1996) Sympathomimetic drugs and adrenergic receptor antagonists. In Goodman and Gilman. The pharmacological basis of therapeutics, 9th ed. McGraw–Hill, New YorkGoogle Scholar
- 6.Ahlquist RP (1948) Am J Physiol 153:586–600Google Scholar
- 20.Pawliszyn J (1997) Solid-phase microextraction: theory and practice. Wiley–VCH, New YorkGoogle Scholar