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Characterisation of B(a)P metabolites formed in an ex vivo pig skin model using three complementary analytical methods

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Abstract

An original method was developed to separate, identify and quantify the different benzo(a)pyrene (B(a)P) metabolites formed through oxidative and conjugative pathways. All B(a)P metabolites were separated by an improved high-performance liquid chromatography method, then detected and quantified relatively by online radioactivity detection. At the same time, metabolite structures were characterised by tandem mass spectrometry using two complementary ionisation modes: electrospray ionisation in the negative mode and atmospheric pressure chemical ionisation in the positive mode. This method was successfully applied to the analysis of B(a)P metabolites, produced by incubation of B(a)P with the ex vivo pig ear skin model. These include glucuronic acid and sulphate conjugates of B(a)P-OHs and B(a)P-diols, as well as direct phase I metabolites: B(a)P-tetrol, B(a)P-diones, B(a)P-catechols, B(a)P-diols and B(a)P-OHs.

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Acknowledgements

The authors would like to thank Sandrine Bruel for technical assistance with pig ear skin explants preparation. The SM3P platform of Paris VI University is thanked for providing access to their FT-MS instruments for exact mass measurements.

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Authors and Affiliations

  1. INRA, UMR 1089 Xénobiotiques INRA-ENVT, 180 chemin de Tournefeuille, BP 93173, Toulouse, 31027, France

    Carine Jacques, Emilien L. Jamin, Elisabeth Perdu, Daniel Zalko & Laurent Debrauwer

  2. Pierre Fabre Dermo-cosmétique, Laboratoire de Pharmacocinétique Cutanée, Allée Camille Soula, BP 74, 31322, Vigoulet, France

    Hélène Duplan & Alain Mavon

Authors
  1. Carine Jacques
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  2. Emilien L. Jamin
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  3. Elisabeth Perdu
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  5. Alain Mavon
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  6. Daniel Zalko
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  7. Laurent Debrauwer
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Correspondence to Laurent Debrauwer.

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Jacques, C., Jamin, E.L., Perdu, E. et al. Characterisation of B(a)P metabolites formed in an ex vivo pig skin model using three complementary analytical methods. Anal Bioanal Chem 396, 1691–1701 (2010). https://doi.org/10.1007/s00216-009-3389-1

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  • DOI: https://doi.org/10.1007/s00216-009-3389-1

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