Analytical and Bioanalytical Chemistry

, Volume 397, Issue 1, pp 147–160 | Cite as

Quantitative LC-ESI-MS/MS metabolic profiling method for fatty acids and lipophilic metabolites in fermentation broths from β-lactam antibiotics production

  • Simone Schiesel
  • Michael Lämmerhofer
  • Wolfgang Lindner
Original Paper


In the present paper, we report on the development of a straightforward reversed-phase liquid chromatography–electrospray ionization–tandem mass spectrometry method for the determination of the most abundant fatty acids; α-tocopherol and cephalosporin P1 in fermentation broths. Using this method, fatty acids could be successfully determined in extracts of fermentation broths from penicillin and cephalosporin production without prior derivatization. Matrix effects were investigated in detail, and various kinds of calibrations (i.e., by use of neat standard solutions as well as by matrix-matched calibration employing standard addition each with and without internal standards) were comparatively assessed. The optimized and validated method was employed for the analysis of extracts of fermentation broths and nutrition media.


Fatty acids α-Tocopherol β-Lactam antibiotics Penicillin HPLC-ESI-MS/MS Metabolic profiling 



Financial support by the Austrian Christian Doppler Research Society and the industry partner Sandoz (Kundl, Austria) is gratefully acknowledged.

Supplementary material

216_2009_3340_MOESM1_ESM.pdf (2.5 mb)
Supplementary data to this article can be found in the online version. (PDF 1999 kb)


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Simone Schiesel
    • 1
  • Michael Lämmerhofer
    • 1
  • Wolfgang Lindner
    • 1
  1. 1.Christian-Doppler Laboratory for Molecular Recognition Materials, Department of Analytical Chemistry and Food ChemistryUniversity of ViennaViennaAustria

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