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Analytical and Bioanalytical Chemistry

, Volume 397, Issue 1, pp 55–62 | Cite as

An immunochemical test for rapid screening of zearalenone and T-2 toxin

  • Evgenia Yu. Basova
  • Irina Yu. Goryacheva
  • Tatiana Yu. Rusanova
  • Natalia A. Burmistrova
  • Richard Dietrich
  • Erwin Märtlbauer
  • Christ’l Detavernier
  • Carlos Van Peteghem
  • Sarah De Saeger
Original Paper

Abstract

An immunochemically based test for non-instrumental simultaneous detection of zearalenone (ZEA) and T-2 toxin (T2) in feed was developed. The method combines clean-up of sample extract, pre-concentration of analytes by immunoextraction and immunodetection through the enzymatic reaction of horseradish peroxidase (HRP). The test is housed inside a standard 1-mL solid-phase extraction column and consists of three layers: two test layers (one for ZEA and another for T2) with immobilised specific antibodies and one control layer with bound anti-HRP antibodies. Feed extract was passed through an additional column with clean-up layer, which was disconnected after extract application. Total assay time was about 15 min for six samples and detection time was 4 min after chromogenic substrate application. Under optimised conditions a cut-off level for ZEA and T2 of 100 µg/kg was established. Different feed types were analysed for ZEA and T2 contamination by the proposed method and results were confirmed by LC-MS/MS.

Figure

An immunochemically-based test for non-instrumental simultaneous detection of zearalenone (ZEA) and T-2 toxin (T2) in feed.

Keywords

Non-instrumental test Visual detection Fusarium toxin Mycotoxin Zearalenone T-2 toxin Feed 

Notes

Acknowledgments

The authors would like to acknowledge the cooperation agreement between Ghent University and Saratov State University. The Belgian federal Science Policy is acknowledged for its financial support; bilateral action; joint Belgian–Chinese project on “Joint study of Fusarium and related mycotoxins in food: detection and control” Bl/02/C46.

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Evgenia Yu. Basova
    • 1
  • Irina Yu. Goryacheva
    • 1
  • Tatiana Yu. Rusanova
    • 1
  • Natalia A. Burmistrova
    • 1
  • Richard Dietrich
    • 2
  • Erwin Märtlbauer
    • 2
  • Christ’l Detavernier
    • 3
  • Carlos Van Peteghem
    • 3
  • Sarah De Saeger
    • 3
  1. 1.Chemistry Faculty, Department of Common and Inorganic ChemistrySaratov State UniversitySaratovRussia
  2. 2.Chair of Hygiene and Technology of MilkLudwig-Maximilians-Universität MünchenOberschleißheimGermany
  3. 3.Faculty of Pharmaceutical Sciences, Laboratory of Food AnalysisGhent UniversityGhentBelgium

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