Analytical and Bioanalytical Chemistry

, Volume 397, Issue 1, pp 55–62 | Cite as

An immunochemical test for rapid screening of zearalenone and T-2 toxin

  • Evgenia Yu. Basova
  • Irina Yu. Goryacheva
  • Tatiana Yu. Rusanova
  • Natalia A. Burmistrova
  • Richard Dietrich
  • Erwin Märtlbauer
  • Christ’l Detavernier
  • Carlos Van Peteghem
  • Sarah De Saeger
Original Paper


An immunochemically based test for non-instrumental simultaneous detection of zearalenone (ZEA) and T-2 toxin (T2) in feed was developed. The method combines clean-up of sample extract, pre-concentration of analytes by immunoextraction and immunodetection through the enzymatic reaction of horseradish peroxidase (HRP). The test is housed inside a standard 1-mL solid-phase extraction column and consists of three layers: two test layers (one for ZEA and another for T2) with immobilised specific antibodies and one control layer with bound anti-HRP antibodies. Feed extract was passed through an additional column with clean-up layer, which was disconnected after extract application. Total assay time was about 15 min for six samples and detection time was 4 min after chromogenic substrate application. Under optimised conditions a cut-off level for ZEA and T2 of 100 µg/kg was established. Different feed types were analysed for ZEA and T2 contamination by the proposed method and results were confirmed by LC-MS/MS.


An immunochemically-based test for non-instrumental simultaneous detection of zearalenone (ZEA) and T-2 toxin (T2) in feed.


Non-instrumental test Visual detection Fusarium toxin Mycotoxin Zearalenone T-2 toxin Feed 



The authors would like to acknowledge the cooperation agreement between Ghent University and Saratov State University. The Belgian federal Science Policy is acknowledged for its financial support; bilateral action; joint Belgian–Chinese project on “Joint study of Fusarium and related mycotoxins in food: detection and control” Bl/02/C46.


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Evgenia Yu. Basova
    • 1
  • Irina Yu. Goryacheva
    • 1
  • Tatiana Yu. Rusanova
    • 1
  • Natalia A. Burmistrova
    • 1
  • Richard Dietrich
    • 2
  • Erwin Märtlbauer
    • 2
  • Christ’l Detavernier
    • 3
  • Carlos Van Peteghem
    • 3
  • Sarah De Saeger
    • 3
  1. 1.Chemistry Faculty, Department of Common and Inorganic ChemistrySaratov State UniversitySaratovRussia
  2. 2.Chair of Hygiene and Technology of MilkLudwig-Maximilians-Universität MünchenOberschleißheimGermany
  3. 3.Faculty of Pharmaceutical Sciences, Laboratory of Food AnalysisGhent UniversityGhentBelgium

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