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Analytical and Bioanalytical Chemistry

, Volume 396, Issue 6, pp 1969–1975 | Cite as

Towards future reference systems for GM analysis

  • Stefanie TrapmannEmail author
  • Philippe Corbisier
  • Heinz Schimmel
  • Hendrik Emons
Trends

Abstract

Despite the fact that the measurement unit for the quantification of GMOs in food and feed products has not yet been unambiguously agreed upon in Europe, international trade requires reliable GMO analysis measuring comparably the GMO content of products. The two reference systems, based either on mass fractions or on copy number ratios, and their metrological traceability chains are presented and discussed. It is concluded that, properly established and expressed, measurement results in copy number ratios can provide a metrologically sound reference system. In this case, certified reference materials used for calibration and quality control can be independent of each other and the uncertainty derived from calibration can correctly be included in the overall uncertainty of the GMO measurement. However, further efforts are required to establish this metrological system.

Keywords

PCR GMO Calibration Traceability Measurement systems Copy number ratio Mass fraction 

Abbreviations

GM

Genetically modified

GMO

Genetically modified organisms

PCR

Polymerase chain reaction

EC

European Commission

CRM

Certified reference material

RSDR

Relative reproducibility standard deviation

DNA

Desoxyribonucleic acid

pDNA

Plasmid DNA

gDNA

Genomic DNA

LOD

Limit of detection

SI

International System of Units

U

Expanded uncertainty

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Stefanie Trapmann
    • 1
    Email author
  • Philippe Corbisier
    • 1
  • Heinz Schimmel
    • 1
  • Hendrik Emons
    • 1
  1. 1.European Commission, Joint Research CentreInstitute for Reference Materials and Measurements (IRMM)GeelBelgium

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