Skip to main content

Advertisement

Log in

New approaches in GMO detection

  • Review
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

The steady rate of development and diffusion of genetically modified plants and their increasing diversification of characteristics, genes and genetic control elements poses a challenge in analysis of genetically modified organisms (GMOs). It is expected that in the near future the picture will be even more complex. Traditional approaches, mostly based on the sequential detection of one target at a time, or on a limited multiplexing, allowing only a few targets to be analysed at once, no longer meet the testing requirements. Along with new analytical technologies, new approaches for the detection of GMOs authorized for commercial purposes in various countries have been developed that rely on (1) a smart and accurate strategy for target selection, (2) the use of high-throughput systems or platforms for the detection of multiple targets and (3) algorithms that allow the conversion of analytical results into an indication of the presence of individual GMOs potentially present in an unknown sample. This paper reviews the latest progress made in GMO analysis, taking examples from the most recently developed strategies and tools, and addresses some of the critical aspects related to these approaches.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Holst-Jensen A, De Loose M, Van Den Eede G (2006) J Agric Food Chem 54:2799–2809

    Article  CAS  Google Scholar 

  2. James C (2008) ISAAA brief no 39. ISAAA, Ithaca

    Google Scholar 

  3. Stein AJ, Rodríguez-Cerezo E (2009) JRC technical report EUR 23486 EN. Office for Official Publications of the European Communities, Luxembourg

    Google Scholar 

  4. Carter CA, Gruère GP (2006) In: Just R, Alston JM, Zilberman D (eds) Regulating agricultural biotechnology. Economics and policies. Springer, New York

  5. European Commission (2001) Off J Eur Communities L 106:1–38

    Google Scholar 

  6. European Commission (2003) Off J Eur Union L 268:1–23

    Google Scholar 

  7. European Commission (2003) Off J Eur Union L 268:24–28

    Google Scholar 

  8. Ramessar K, Capell T, Twyman RM, Quemada H, Christou P (2008) Nat Biotechnol 26:975–978

    Article  CAS  Google Scholar 

  9. Querci M, Paoletti C, Van den Eede G (2007) In: Craig W (ed) Collection of biosafety reviews. International Centre for Genetic Engineering and Biotechnology, Trieste

  10. Miraglia M, Berdal KG, Brera C, Corbisier P, Holst-Jensen A, Kok E, Marvin HJP, Schimmel H, Rentsch J, van Rie JP, Zagon J (2004) Food Chem Toxicol 42:1157–1180

    Article  CAS  Google Scholar 

  11. Holst-Jensen A (2007) In: Pico Y (ed) Food toxicants analysis. Techniques, strategies and developments. Elsevier, Amsterdam

    Google Scholar 

  12. Rodríguez-Lázaro D, Lombard B, Smith H, Rzezutka A, D’Agostino M, Helmuth R, Schroeter A, Malorny B, Miko A, Guerra B, Davison J, Kobilinsky A, Hernández M, Bertheau Y, Cook N (2007) Trends Food Sci Technol 18:306–319

    Article  Google Scholar 

  13. Holst-Jensen A, Berdal KG (2004) J AOAC Int 87:927–936

    CAS  Google Scholar 

  14. BATS (2003) Genetically modified (GM) crops: molecular and regulatory details. version 2. BATS Centre for Biosafety and Sustainability. http://www.bats.ch/gmo-watch/GVO-report140703.pdf. Accessed July 2009

  15. Fernandez S, Charles-Delobel C, Geldreich A, Berthier G, Boyer F, Collonnier C, Coué-Philippe G, Diolez A, Duplan M, Kebdani N, Romaniuk M, Feinberg M, Bertheau Y (2005) J AOAC Int 88(2):547–557

    CAS  Google Scholar 

  16. Morisset D, Demšar T, Gruden K, Vojvoda J, Štebih D, Žel J (2009) Nat Biotechnol 27:700–701

    Article  CAS  Google Scholar 

  17. Dong W, Yang L, Shen K, Kim B, Kleter GA, Marvin HJP, Guo R, Liang W, Zhang D (2008) BMC Bioinformatics 9:260. doi:10.1186/1471-2105-9-260

    Article  Google Scholar 

  18. Elenis DS, Kalogianni DP, Glynou K, Ioannou PC, Christopoulos TK (2008) Anal Bioanal Chem 392:347–354

    Article  CAS  Google Scholar 

  19. Marmiroli N, Maestri E, Gullì M, Malcevschi A, Peano C, Bordoni R, De Bellis G (2008) Anal Bioanal Chem 392:369–384

    Article  CAS  Google Scholar 

  20. Michelini E, Simoni P, Cevenini L, Mezzanotte L, Roda A (2009) Anal Bioanal Chem 392:355–367

    Article  Google Scholar 

  21. Holst-Jensen A, Rønning SB, Løvseth A, Berdal KG (2003) Anal Bioanal Chem 375:985–993

    CAS  Google Scholar 

  22. Van den Bulcke M, Lievens A, Barbeau-Piednoir E, Mbongolo Mbella G, Roosens N, Sneyers M, Casi AL (2009) Anal Bioanal Chem (in press)

  23. Kralj Novak P, Gruden K, Morisset K, Lavrač N, Štebih D, Rotter A, Žel J (2009) J AOAC Int (in press)

  24. Xu J, Miao H, Wu H, Huang W, Tang R, Qiu M, Wen J, Zhu S, Li Y (2006) Biosens Bioelectron 22:71–77

    Article  CAS  Google Scholar 

  25. Waiblinger H-U, Boernsen B, Pietsch K (2008) Dtsch Lebensm Rundsch 104(6):261–264

    CAS  Google Scholar 

  26. Waiblinger HU, Ernst B, Anderson A, Pietsch K (2007) Eur Food Res Technol 226:1221–1228

    Article  Google Scholar 

  27. Länderausschusses Gentechnik (2006) Real-Time PCR zur quantitativen Bestimmung gentechnisch veränderter Rapslinien mit dem 35S/pat-Genkonstrukt. http://www.lag-gentechnik.de/dokumente/SOP_UAM_pat_quant_28032006.pdf

  28. Grohmann L, Brünen-Nieweler C, Nemeth A, Waiblinger HU (2009) J Agric Food Chem 57(19):8913–8920

    Article  CAS  Google Scholar 

  29. Reiting R, Broll H, Waiblinger HU, Grohmann L (2007) J Verbr Lebensm 2:116–121

    Article  CAS  Google Scholar 

  30. Hamels S, Glouden T, Gillard K, Mazzara M, Debode F, Foti N, Sneyers M (2009) Esteve Nuez T, Pla M, Berben G, Moens W, Bertheau Y, Audéon C, Van den Eede G, Remacle J. Eur Food Res Technol 228:531–541

    Article  CAS  Google Scholar 

  31. International Organization for Standardization (1994) International Standard (ISO) 5725. Accuracy (trueness and precision) of measurement methods and results. International Organization for Standardization, Geneva

    Google Scholar 

  32. Querci M, Foti N, Bogni A, Kluga L, Broll H, Van den Eede G (2009) Food. Anal Methods. doi:10.1007/s12161-009-9093-0

    Google Scholar 

  33. Mano J, Shigemitsu N, Futo S, Akiyama H, Teshima R, Hino A, Furui S, Kitta K (2009) J Agric Food Chem 57:26–37

    Article  CAS  Google Scholar 

  34. Permingeat HR, Reggiardo MI, Vallejos RH (2002) J Agric Food Chem 50:4431–4436

    Article  CAS  Google Scholar 

  35. Germini A, Zanetti A, Salati C, Rossi S, Forre C, Schmid S, Marchelli R, Fogher C (2004) J Agric Food Chem 52:3275–3280

    Article  CAS  Google Scholar 

  36. Hernández M, Rodríguez-Lázaro D, Zhang D, Esteve T, Pla M, Prat S (2005) J Agric Food Chem 53:3333–3337

    Article  Google Scholar 

  37. Germini A, Rossi S, Zanetti A, Corradini R, Fogher C, Marchelli R (2005) J Agric Food Chem 53:3958–3962

    Article  CAS  Google Scholar 

  38. Bordoni R, Germini A, Mezzelani A, Marchelli R, De Bellis G (2005) J Agric Food Chem 53:912–918

    Article  CAS  Google Scholar 

  39. Leimanis S, Hernández M, Fernández S, Boyer F, Burns M, Bruderer S, Glouden T, Harris N, Kaeppeli O, Philipp P, Pla M, Puigdomènech P, Vaitilingom M, Bertheau Y, Remacle J (2006) Plant Mol Biol 61:123–139

    Article  CAS  Google Scholar 

  40. Leimanis S, Hamels S, Nazé F, Mbongolo Mbella G, Sneyers M, Hochegger R, Broll H, Roth L, Dallmann K, Micsinai A, La Paz JL, Pla M, Brünen-Nieweler C, Papazova N, Taverniers I, Hess N, Kirschneit B, Bertheau Y, Audeon C, Laval V, Busch U, Pecoraro S, Neumann K, Rösel S, van Dijk J, Kok E, Bellocchi G, Foti N, Mazzara M, Moens W, Remacle J, Van den Eede G (2008) Eur Food Res Technol 227:1621–1632

    Article  CAS  Google Scholar 

  41. Prins TW, van Dijk JP, Beenen HG, Van Hoef AMA, Voorhuijzen MM, Schoen CD, Aarts HJM, Kok EJ (2008) BMC Genomics 9:584

    Article  Google Scholar 

  42. Morisset D, Stebih D, Cankar K, Žel J, Gruden K (2008) Eur Food Res Technol 227:1287–1297

    Article  CAS  Google Scholar 

  43. Morisset D, Dobnik D, Hamels S, Žel J, Gruden K (2008) Nucleic Acids Res 36(18):e118. doi:10.1093/nar/gkn524

    Article  Google Scholar 

  44. Dobnik D, Morisset D, Gruden K (2009) Anal Bioanal Chem (in press)

  45. Fantozzi A, Ermolli M, Marini M, Balla B, Querci M, Van den Eede G (2008) Food Anal Methods 1:10–17

    Article  Google Scholar 

  46. Nadal A, Coll A, La Paz JL, Esteve T, Pla M (2006) Electrophoresis 27:3879–3888

    Article  CAS  Google Scholar 

  47. Nadal A, Esteve T, Pla M (2009) J AOAC Int 92(3):765–777

    CAS  Google Scholar 

  48. Bellocchi G, Acutis M, Paoletti C, Confalonieri R, Trevisiol P, Grazioli E (2008) Charles Delobel C, Savini C, Mazzara M, Van den Eede G. Food Anal Methods 1:126–135

    Article  Google Scholar 

Download references

Acknowledgements

The authors would like to thank Kristina Gruden and Dany Morisset from the National Institute of Biology for their valuable inputs to the manuscript.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Maddalena Querci.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Querci, M., Van den Bulcke, M., Žel, J. et al. New approaches in GMO detection. Anal Bioanal Chem 396, 1991–2002 (2010). https://doi.org/10.1007/s00216-009-3237-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-009-3237-3

Keywords

Navigation