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Determination of human urinary kanamycin in one step using urea-enhanced surface plasmon resonance light-scattering of gold nanoparticles

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Abstract

The purpose of this study was to establish a simple, sensitive analytical method for kanamycin (KANA) in human urine. Enhancement of the plasmon resonance light-scattering (PRLS) of gold nanoparticles (AuNPs) by KANA provided the basis for this analytical method. At pH 6.7, KANA induced AuNPs aggregation with enhanced PRLS. The PRLS of the AuNPs–KANA system was further enhanced by addition of urea. The linear range and detection limit for KANA were from 20–800 nmol L−1 and 2 nmol L−1, respectively. Potential interfering substances present in urine had a negligible effect on the determination, thus preliminary sample separations were not necessary. Recovery of KANA from spiked human urine was 94–104%. This simple, sensitive method, using urea to enhance the PRLS of the AuNPs–KANA system, may provide a new approach for determination of compounds rich in OH groups.

Schematic diagram of KANA-induced aggregation of AuNPs and enhancement by urea, including magnification for illustration of N···H-O hydrogen bond between KANA and urea

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Abbreviations

PRLS:

Plasmon resonance light-scattering

AuNPs:

Gold nanoparticles

KANA:

Kanamycin

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (no. 20975004 and 90713013) and the Instrumental Analysis Fund of Peking University.

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Authors and Affiliations

  1. The Key Laboratory of Bioorganic Chemistry and Molecular Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China

    Xinyi Wang, Mingjian Zou, Xiao Xu, Rong Lei, Kean Li & Na Li

  2. College of Sciences, Shenyang Agricultural University, Shenyang, 110161, China

    Xinyi Wang

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  1. Xinyi Wang
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  2. Mingjian Zou
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  3. Xiao Xu
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  4. Rong Lei
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  5. Kean Li
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  6. Na Li
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Correspondence to Na Li.

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Wang, X., Zou, M., Xu, X. et al. Determination of human urinary kanamycin in one step using urea-enhanced surface plasmon resonance light-scattering of gold nanoparticles. Anal Bioanal Chem 395, 2397–2403 (2009). https://doi.org/10.1007/s00216-009-3134-9

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  • DOI: https://doi.org/10.1007/s00216-009-3134-9

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