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Depolymerization study of sodium hyaluronate by flow field-flow fractionation/multiangle light scattering

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Abstract

Thermal depolymerization of ultrahigh-molecular-weight (UHMW) sodium hyaluronate (NaHA) was studied systematically by using frit-inlet asymmetrical flow field-flow fractionation/multiangle light scattering/differential refractive index (FI-AFlFFF/MALS/DRI). FI-AFlFFF was utilized for the size separation of NaHA samples which had been thermally degraded for varied treatment times, followed by light-scattering detection to determine MW and structural information of degraded NaHA products. Analysis of NaHA products showed time-dependent depolymerization of raw molecules into smaller-MW components, as well as unfolding of compact structures of UHMW NaHA. To determine whether the observed decrease in MW of sodium hyaluronate originated from the chain degradation of UHMW molecules or from dissociation of entangled complex particles that may have been formed by intermolecular association, narrow size fractions (1 × 107–6 × 107 and >6 × 107 MW) of NaHA molecules were collected during FlFFF separation and followed by thermal treatment. Subsequent FI-AFlFFF/MALS analysis of collected fractions after thermal treatment suggested that the ultrahigh-MW region (>107 Da) of NaHA is likely to result from supermolecular structures formed by aggregation of large molecules.

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References

  1. Cortivo R, Brun P, Rastrelli A, Abatangelo G (1991) Biomaterials 12:727–730

    Article  CAS  Google Scholar 

  2. Prestwitch GD, Marecak DM, Marecek JF, Vercurysse KP, Ziebel MR (1998) J Controlled Release 53:93–103

    Article  Google Scholar 

  3. Iqbal Z, Midgley JM, Watson DG, Karditsas SD, Dutton GN, Wilson W (1997) Pharmacy World & Science 19:246–250

    Article  CAS  Google Scholar 

  4. Vercruysse KP, Prestwich GD (1998) Crit Rev Ther Drug Carrier Syst 15:513–555

    CAS  Google Scholar 

  5. Sasari H, Konttinen TY, Santavirta S (1989) Med Sci Res 18:99–101

    Google Scholar 

  6. Takahashi R, Al-Assaf S, Williams PA, Kubota K, Okamoto A, Nishinari K (2003) Biomacromolecules 4:404–409

    Article  CAS  Google Scholar 

  7. Mengher LS, Pandher KS, Bron AJ, Davey CC (1986) Br J Opthalmol 70:442–447

    Article  CAS  Google Scholar 

  8. Miyazaki S, Yomota C, Okada S (1996) J Ocul Pharmacol 12:27

    Article  Google Scholar 

  9. Yeung B, Marecak D (1999) J Chromatogr A 852:573–581

    Article  CAS  Google Scholar 

  10. Rehakova M, Bakos D, Soldan M, Vizarova K (1994) Int J Biol Macromol 16:121–124

    Article  CAS  Google Scholar 

  11. Sall I, Ferad G (2007) Polym Degrad Stab 92:915–919

    Article  CAS  Google Scholar 

  12. Ynag BY, Montgomery R (2007) Bioresour Tech 98:3084–3089

    Article  CAS  Google Scholar 

  13. Soltes L, Mendichi R, Kogan G, Schiller J, Stankovska M, Arnhold J (2006) Biomacromolecules 7:659–668

    Article  CAS  Google Scholar 

  14. Cleland RL (1977) Arch Biochem Biophys 180:57–68

    Article  CAS  Google Scholar 

  15. Livant P, Roden L, Krishna R (1992) Carbohydr Res 237:271281

    Article  Google Scholar 

  16. Sheehan JK, Arundel C, Phelps CF (1983) Int J Biol Macromol 5:222–228

    Article  CAS  Google Scholar 

  17. Giddings JC (1981) Anal Chem 53:1170A–1178A

    Article  CAS  Google Scholar 

  18. Giddings JC (1993) Science 260:1456–1465

    Article  CAS  Google Scholar 

  19. Wahlund K-G, Giddings JC (1987) Anal Chem 59:1332–1339

    Article  CAS  Google Scholar 

  20. Ratanathanawongs SK, Giddings JC (1992) Anal Chem 64:6–15

    Article  CAS  Google Scholar 

  21. Kim H, Lee H, Moon MH (2006) Bull Korean Chem Soc 27:413–418

    Article  CAS  Google Scholar 

  22. Motohashi N, Nakamichi Y, Mori I, Nishikawa H, Umemoto K (1988) J Chromatogr 435:335–342

    Article  CAS  Google Scholar 

  23. Hecker B, Fawell PD, Jefferson A, Farrow JB (1999) J Chromatogr A 837:139–151

    Article  CAS  Google Scholar 

  24. Wittgren B, Wahlund K-G (1997) J Chromatogr A 791:135–149

    Article  CAS  Google Scholar 

  25. Picton L, Bataille I, Muller G (2000) Carbohydr Polym 42:23–31

    Article  CAS  Google Scholar 

  26. Duval C, Cerf DL, Picton L, Muller G (2001) J Chromatogr B 753:115–122

    Article  CAS  Google Scholar 

  27. Shin DY, Hwang EJ, Cho I-H, Moon MH (2007) J Chromatogr A 1160:270–275

    Article  CAS  Google Scholar 

  28. Moon MH, Shin DY, Lee N, Hwang E, Cho I-H (2008) J Chromatogr B 864:15–21

    Article  CAS  Google Scholar 

  29. Lee H, Kim H, Moon MH (2005) J Chromatography A 1089:203–210

    Article  CAS  Google Scholar 

  30. Lee H, Cho I-H, Moon MH (2006) J Chromatography A 1131:185–191

    Article  CAS  Google Scholar 

  31. Moon MH, Kwon HS, Park I (1997) Anal Chem 69:1436–1440

    Article  CAS  Google Scholar 

  32. Moon MH, Williams PS, Kwon H (1999) Anal Chem 71:2657–2666

    Article  CAS  Google Scholar 

  33. Moon MH, Kang D, Hwang I, Williams PS (2002) J Chromatogr A 955:263–272

    Article  Google Scholar 

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Acknowledgements

This study was supported by the Korea Science and Engineering Foundation through the Center for Bioactive Molecular Hybrids (CBMH) at Yonsei University and in part by Shinpoong Pharmaceutical, Co. Ltd.

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Authors and Affiliations

  1. Department of Chemistry, Yonsei University, Seoul, 120-749, South Korea

    Ji Hye Kwon & Myeong Hee Moon

  2. Department of Biotechnology, Shinpoong Pharmaceutical, Co. Ltd., Ansan, Kyeonggi-Do, South Korea

    Euijin Hwang & Il-Hwan Cho

Authors
  1. Ji Hye Kwon
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  2. Euijin Hwang
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  3. Il-Hwan Cho
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  4. Myeong Hee Moon
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Correspondence to Myeong Hee Moon.

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Kwon, J.H., Hwang, E., Cho, IH. et al. Depolymerization study of sodium hyaluronate by flow field-flow fractionation/multiangle light scattering. Anal Bioanal Chem 395, 519–525 (2009). https://doi.org/10.1007/s00216-009-2952-0

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  • DOI: https://doi.org/10.1007/s00216-009-2952-0

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