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Potential-modulated fluorescence spectroscopy of the membrane potential-sensitive dye di-4-ANEPPS at the 1,2-dichloroethane/water interface

Analytical and Bioanalytical Chemistry volume 395, pages 1055–1061 (2009)Cite this article

Abstract

The spectrofluorometric behavior of a membrane potential-sensitive dye, 1-(3-sulfonatopropyl)-4-[β-{2-(di-N-butylamino)-6-naphthyl}vinyl]pyridinium betaine (di-4-ANEPPS), at the polarized 1,2-dichloroethane/water interface was studied by means of potential-modulated fluorescence (PMF) spectroscopy. The results, combined with those from cyclic and alternating current voltammetry, clearly suggested that the dye adsorbed at the interface underwent a reorientation with increasing the interfacial potential, giving a well-developed PMF response as well as a voltammetric response. In addition to the PMF response, another PMF response was observed by addition of dilauroyl phosphatidylcholine (DLPC). This additional response was well explained in terms of a reorientation of di-4-ANEPPS at the interface, which would be induced by the potential-dependent desorption of DLPC from the interface. Thus, the present study supported the reorientation/solvatochromic mechanism for the membrane potential-sensitive dye rather than the electrochromic mechanism.

Reaction model for the potential-induced reorientation of di-4-ANEPPS at the O/W interface in the presence of DLPC in the DCE phase.

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References

  1. Fluhler E, Burnham VG, Loew LM (1985) Biochemistry 24:5749

    Article  CAS  Google Scholar 

  2. Montana V, Farkas DL, Loew LM (1989) Biochemistry 28:4536

    Article  CAS  Google Scholar 

  3. Loew LM (1996) Pure Appl Chem 68:1405

    Article  CAS  Google Scholar 

  4. Clark RJ, Zouni A, Holzwarth JF (1995) Biophys J 68:1406

    Article  Google Scholar 

  5. Müller W, Windisch H, Tritthart HA (1986) Eur Biophys J 14:103

    Article  Google Scholar 

  6. Jones MA, Bohn PW (2000) Anal Chem 72:3776

    Article  CAS  Google Scholar 

  7. Jones MA, Bohn PW (2001) J Phys Chem B 105:2197

    Article  CAS  Google Scholar 

  8. Nagatani H, Iglesias RA, Fermín DJ, Brevet PF, Girault HH (2000) J Phys Chem B 104:6869

    Article  CAS  Google Scholar 

  9. Nagatani H, Fermín DJ, Girault HH (2001) J Phys Chem B 105:9463

    Article  CAS  Google Scholar 

  10. Nakatani K, Nagatani H, Fermín DJ, Girault HH (2002) J Electroanal Chem 518:1

    Article  CAS  Google Scholar 

  11. Nagatani H, Suzuki S, Fermín DJ, Girault HH, Nakatani K (2006) Anal Bioanal Chem 386:633

    Article  CAS  Google Scholar 

  12. Nagatani H, Ozeki T, Osakai T (2006) J Electroanal Chem 588:99

    Article  CAS  Google Scholar 

  13. Osakai T, Yamada H, Nagatani H, Sagara T (2007) J Phys Chem C 111:9480

    Article  CAS  Google Scholar 

  14. Nagatani H, Sagara T (2007) Anal Sci 23:1041

    Article  CAS  Google Scholar 

  15. Bard AJ, Inzelt G, Scholz F (eds) (2008) Electrochemical dictionary. Springer, Berlin, pp 284–285

    Google Scholar 

  16. Osakai T, Kakutani T, Senda M (1984) Bull Chem Soc Jpn 57:370

    Article  CAS  Google Scholar 

  17. Kakutani T, Senda M (1979) Bull Chem Soc Jpn 52:3236

    Article  CAS  Google Scholar 

  18. Kakiuchi T, Yamane M, Osakai T, Senda M (1987) Bull Chem Soc Jpn 60:4223

    Article  CAS  Google Scholar 

  19. Kakiuchi T (1996) In: Volkov AG, Deamer DW (eds) Liquid–liquid interfaces, theory and methods. CRC, Boca Raton, pp 317–331

    Google Scholar 

  20. Yoshida Y, Maeda K, Shirai O (2005) J Electroanal Chem 578:17

    Article  CAS  Google Scholar 

Download references

Acknowledgements

One of the authors (T. Osakai) acknowledges discussions with Emeritus Professor Mitsugi Senda of Kyoto University.

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Affiliations

  1. Department of Chemistry, Graduate School of Science, Kobe University, Nada, Kobe, 657-8501, Japan

    Toshiyuki Osakai & Junpei Sawada

  2. Department of Applied Chemistry, Faculty of Engineering, Nagasaki University, Bunkyo, Nagasaki, 852-8521, Japan

    Hirohisa Nagatani

Authors
  1. Toshiyuki Osakai
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  2. Junpei Sawada
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  3. Hirohisa Nagatani
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Corresponding author

Correspondence to Toshiyuki Osakai.

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Reaction model for the potential-induced reorientation of di-4-ANEPPS at the 1,2-dichloroethane (DCE)/water interface in the presence of DLPC in the DCE phase (PDF 287 kb)

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Osakai, T., Sawada, J. & Nagatani, H. Potential-modulated fluorescence spectroscopy of the membrane potential-sensitive dye di-4-ANEPPS at the 1,2-dichloroethane/water interface. Anal Bioanal Chem 395, 1055–1061 (2009). https://doi.org/10.1007/s00216-009-2915-5

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  • DOI: https://doi.org/10.1007/s00216-009-2915-5

Keywords

  • Membrane potential-sensitive dye
  • di-4-ANEPPS
  • Liquid/liquid interface
  • Potential-modulated fluorescence