Skip to main content
SpringerLink
Log in

The effect of heat treatment on the detection of peanut allergens as determined by ELISA and real-time PCR

  • Original Paper
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

Peanut allergic reactions can result from the ingestion of even very small quantities of peanut and represent a severe threat to the health of sensitised individuals. The detection of peanut traces in food products is therefore of prime importance. Peanut traces which can be (unintentionally) present in food products have usually undergone one or more processing steps like roasting and baking. Therefore, methods designed to detect such traces have to be capable of detecting heat-treated peanuts. Commonly used methodologies designed to detect peanut traces in food products are enzyme-linked immunosorbent assays (ELISAs) that detect peanut-specific proteins, and polymerase-chain-reaction (PCR)-based methods targeting peanut-specific DNA. A comparative analysis of such methods was performed and the impact of heat treatment on peanut kernels as well as the impact on a peanut-containing food matrix are investigated. Our results show that heat treatments have a detrimental effect on the detection of peanut with either type of method and that both types of methods are affected in a similar manner.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Sicherer SH, Muñoz-Furlong A, Murphy R, Wood RA, Sampson HA (2003) Pediatrics 111:1591–1594

    Google Scholar 

  2. Sicherer SH, Muñoz-Furlong A, Sampson HA (2004) J Allergy Clin Immunol 114:159–165

    Article  Google Scholar 

  3. Helm RM, Burks AW (2000) Curr Opin Immunol 12:647–653

    Article  CAS  Google Scholar 

  4. Ortolani C, Ispano M, Scibilia J, Pastorello EA (2001) Allergy 56(67):5–8

    Article  Google Scholar 

  5. Sampson HA (2003) Pediatrics 111(6 III):1601–1608

    Google Scholar 

  6. Bock SA, Munoz-Furlong A, Sampson HA (2001) J Allergy Clin Immunol 107(1):191–193

    Article  CAS  Google Scholar 

  7. Pumphrey R (2001) Food Allergy Intolerance 2:91–104

    Google Scholar 

  8. European Parliament and Council (2000) Official J Europ Commun L109:29–42

    Google Scholar 

  9. European Parliament and Council (2007) Official J Europ Commun L310:11–14

    Google Scholar 

  10. Maleki SJ, Chung SY, Champagne ET, Raufman JP (2000) J Allergy Clin Immunol 106(4):763–768

    Article  CAS  Google Scholar 

  11. Beyer K, Morrow E, Li XM, Bardina L, Bannon GA, Burks AW et al (2001) J Allergy Clin Immunol 107:1077–1081

    Article  CAS  Google Scholar 

  12. Chung SY, Champagne E (2001) J Agric Food Chem 49:3911–3916

    Article  CAS  Google Scholar 

  13. Chassaigne H, Brohée M, Nørgaard JV, van Hengel AJ (2007) Food Chem 105:1671–1681

    Article  CAS  Google Scholar 

  14. Mondoulet L, Paty E, Drumare MF, Ah-Leung S, Scheinmann P, Willemot RM, Wal JM, Bernard H (2005) J Agric Food Chem 53(11):4547–4553

    Article  CAS  Google Scholar 

  15. Poms RE, Capelletti C, Anklam E (2004) Mol Nutr Food Res 48:459–464

    Article  CAS  Google Scholar 

  16. Krska R, Welzig E, Baumgartner S (2004) Anal Bioanal Chem 378:63–65

    Article  CAS  Google Scholar 

  17. Hird H, Lloyd J, Goodier R, Brown J, Reece P (2003) Eur Food Res Technol 217:265–268

    Article  CAS  Google Scholar 

  18. Stephan O, Vieths S (2004) J Agric Food Chem 52:3754–3760

    Article  CAS  Google Scholar 

  19. Sforza S, Scaravelli E, Corradini R, Marchelli R (2005) Chirality 17:515–521

    Article  CAS  Google Scholar 

  20. Rossi S, Scaravelli E, Germini A, Corradini R, Marchelli R (2006) Eur Food Res Technol 223:1–6

    Article  CAS  Google Scholar 

  21. Watanabe T, Akiyama H, Maleki S, Yamakawa H, Iijima K, Yamazaki F, Matsumoto T, Futo S, Arakawa F, Watai M, Maitani T (2006) J Food Biochem 30:215–233

    Article  CAS  Google Scholar 

  22. Scaravelli E, Brohée M, Marchelli R, van Hengel AJ (2008) Eur Food Res Technol 227:857–869

    Article  CAS  Google Scholar 

  23. Piknova L, Pangallo D, Kuchta T (2008) Eur Food Res Technol 226:1155–1158

    Article  CAS  Google Scholar 

  24. Miller JN, Miller JC (2005) Statistics and chemometrics for analytical chemistry. Pearson, England

    Google Scholar 

  25. Burns JM, Valdivia H, Harris N (2004) Anal Bioanal Chem 378:1616–1623

    Article  CAS  Google Scholar 

  26. Whitaker TB, Williams KM, Trucksess MW, Slate AB (2005) J AOAC Int 88(1):161–174

    CAS  Google Scholar 

  27. Koch P, Schäppi GF, Poms RE, Wüthrich B, Anklam E, Battaglia R (2003) Food Addit Contam 20(9):797–803

    Article  CAS  Google Scholar 

  28. Kopper RA, Odum NJ, Sen M, Helm RM, Stanley JS, Burks AW (2005) Int Arch Allergy Immunol 136:16–22

    Article  CAS  Google Scholar 

  29. Bauer T, Weller P, Hammes WP, Hertel C (2003) Eur Food Res Technol 217:338–343

    Article  CAS  Google Scholar 

  30. Bauer T, Weller P, Hammes WP, Haase NU, Hertel C (2004) Environ Biosafety Res 3:215–223

    Article  CAS  Google Scholar 

  31. Moreano F, Busch U, Engel KH (2005) J Agric Food Chem 53:9971–9979

    Article  CAS  Google Scholar 

  32. Engel KH, Moreano F, Ehlert A, Busch U (2006) Trends Food Sci Technol 17:490–497

    Article  CAS  Google Scholar 

  33. European Network of GMO Laboartories (2005) Definition of minimum performance requirements for analytical methods of GMO testing, http://gmo-crl.jrc.it/guidancesdocs.htm

  34. Chen Y, Wang Y, Ge Y, Xu B (2005) J Agric Food Chem 53:10239–10243

    Article  CAS  Google Scholar 

  35. Murray SR, Butler RC, Hardacre AK, Timmerman-Vaughan GM (2007) J Agric Food Chem 55:2231–2239

    Article  CAS  Google Scholar 

  36. Maleki SJ, Hurlburt BK (2004) J AOAC Int 87(6):1475–1479

    CAS  Google Scholar 

  37. Burns JM, Valdivia H (2008) Eur Food Res Technol 226:1513–1524

    Article  CAS  Google Scholar 

  38. Hiramoto K, Kido K, Kikugawa K (1994) J Agric Food Chem 42:689–694

    Article  CAS  Google Scholar 

  39. Koppelman SJ, Vlooswijk RAA, Knippels LMJ, Hessing M, Knol EF, Van Reijsen FC, Bruijnzeel-Koomen CAFM (2001) Allergy 56(2):132–137

    Article  CAS  Google Scholar 

  40. Poms RE, Agazzi ME, Bau A, Brohée M, Capelletti C, Nørgaard JV, Anklam E (2005) Food Addit Contam 22:104–112

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Elena Scaravelli

    Present address: European Food Safety Authority, Largo N. Palli 5/N, 43100, Parma, Italy

Authors and Affiliations

  1. European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Food Safety and Quality Unit, Retieseweg 111, B-2440, Geel, Belgium

    Elena Scaravelli, Marcel Brohée & Arjon J. van Hengel

  2. Universitá degli Studi di Parma, Parco Area delle Scienze 17/a, 43100, Parma, Italy

    Elena Scaravelli & Rosangela Marchelli

Authors
  1. Elena Scaravelli
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Marcel Brohée
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rosangela Marchelli
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Arjon J. van Hengel
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Arjon J. van Hengel.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Scaravelli, E., Brohée, M., Marchelli, R. et al. The effect of heat treatment on the detection of peanut allergens as determined by ELISA and real-time PCR. Anal Bioanal Chem 395, 127–137 (2009). https://doi.org/10.1007/s00216-009-2849-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-009-2849-y

Keywords

Search

Navigation