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Enhanced electrophoretic DNA separation in photonic crystal fiber

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Abstract

Joule heating generated by the electrical current in capillary electrophoresis leads to a temperature gradient along the separation channel and consequently affects the separation quality. We describe a method of reducing the Joule heating effect by incorporating photonic crystal fiber into a micro capillary electrophoresis chip. The photonic crystal fiber consists of a bundle of extremely narrow hollow channels, which ideally work as separation columns. Electrophoretic separation of DNA fragments was simultaneously but independently carried out in 54 narrow capillaries with a diameter of 3.7 μm each. The capillary bundle offers more efficient heat dissipation owing to the high surface-to-volume ratio. Under the same electrical field strength, notable improvement in resolution was obtained in the capillary bundle chip.

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Authors and Affiliations

  1. National Institute of Education, Nanyang Technological University, 1 Nanyang Walk, Singapore, 637616, Singapore

    Yi Sun & Yien Chian Kwok

  2. School of Mechanical and Aerospace Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798, Singapore

    Nam-Trung Nguyen

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  1. Yi Sun
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  2. Nam-Trung Nguyen
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  3. Yien Chian Kwok
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Correspondence to Yien Chian Kwok.

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Sun, Y., Nguyen, NT. & Kwok, Y.C. Enhanced electrophoretic DNA separation in photonic crystal fiber. Anal Bioanal Chem 394, 1707–1710 (2009). https://doi.org/10.1007/s00216-009-2833-6

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  • DOI: https://doi.org/10.1007/s00216-009-2833-6

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