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Simultaneous quantification of buprenorphine, norbuprenorphine, buprenorphine glucuronide, and norbuprenorphine glucuronide in human placenta by liquid chromatography mass spectrometry

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Abstract

A LCMS method was developed and validated for the determination of buprenorphine (BUP), norbuprenorphine (NBUP), buprenorphine glucuronide (BUP-Gluc), and norbuprenorphine glucuronide (NBUP-Gluc) in placenta. Quantification was achieved by selected ion monitoring of m/z 468.4 (BUP), 414.3 (NBUP), 644.4 (BUP-Gluc), and 590 (NBUP-Gluc). BUP and NBUP were identified monitoring MS2 fragments m/z 396, 414 and 426 for BUP, and 340, 364 and 382 for NBUP, and glucuronide conjugates monitoring MS3 fragments m/z 396 and 414 for BUP-Gluc, and 340 and 382 for NBUP-Gluc. Linearity was 1–50 ng/g. Intra-day, inter-day and total assay imprecision (% RSD) were <13.4%, and analytical recoveries were 96.2–113.1%. Extraction efficiencies ranged from 40.7–68%, process efficiencies 38.8–70.5%, and matrix effect 1.3–15.4%. Limits of detection were 0.8 ng/g for all compounds. An authentic placenta from an opioid-dependent pregnant woman receiving BUP pharmacotherapy was analyzed. BUP was not detected but metabolite concentrations were NBUP-Gluc 46.6, NBUP 15.7 and BUP-Gluc 3.2 ng/g.

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Acknowledgments

This research was supported by the National Institutes of Health, Intramural Research Program, National Institute on Drug Abuse, and funding for Marta Concheiro, Ph.D. from the Ministerio de Educación y Ciencia of Spain (Grant number EX-2007-1194). The authors would like to thank Drs. Fred Askin, Gerrun March, and Leigh Ruane from Johns Hopkins Bayview Medical Center (Baltimore, MD) for their help in providing placenta samples.

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Correspondence to Marilyn A. Huestis.

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Concheiro-Guisan, M., Shakleya, D.M. & Huestis, M.A. Simultaneous quantification of buprenorphine, norbuprenorphine, buprenorphine glucuronide, and norbuprenorphine glucuronide in human placenta by liquid chromatography mass spectrometry. Anal Bioanal Chem 394, 513–522 (2009). https://doi.org/10.1007/s00216-009-2706-z

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  • DOI: https://doi.org/10.1007/s00216-009-2706-z

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