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Comparison of off- and in-line solid-phase extraction for enhancing sensitivity in capillary electrophoresis using ochratoxin as a model compound

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Abstract

This paper proposes and compares two approaches based on off- and in-line solid-phase extraction (SPE), intended to enhance sensitivity in capillary electrophoresis with ultraviolet detection (CE-UV) using as a model the determination of ochratoxin A (OA) in river water samples. In the off-line SPE mode, the reversed-phase sorbent (octadecilsylane, C18) selectively retains the target analyte (OA) and allows large volumes of the sample (70 mL) to be introduced and subsequently eluted in a small volume (0.1 mL) of an appropriate solution. In the in-line SPE mode, a custom-made microcartridge is inserted near the inlet of the capillary, which is filled with the same C18 sorbent. This solid phase selectively retains OA present in a sample volume as low as approximately 640 µL for subsequent elution with ca. 135 nL of an appropriate eluent. The limit of detection (LOD) obtained with the in-line SPE method was 1 ng L-1, which is 3 orders of magnitude lower than that obtained with CE-UV and roughly 1 order lower than that provided by the off-line SPE-CE-UV method.

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Acknowledgment

This work was funded by DGI, Spain's Ministry of Science and Technology, within the framework of Project CTQ2007-60426.

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Authors and Affiliations

  1. Department of Analytical Chemistry, University of Córdoba, Campus de Rabanales, 14071, Córdoba, Spain

    S. Almeda, L. Arce & M. Valcárcel

  2. Department of Analytical Chemistry, University of Barcelona, 08028, Barcelona, Spain

    F. Benavente, V. Sanz-Nebot & J. Barbosa

Authors
  1. S. Almeda
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  2. L. Arce
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  3. F. Benavente
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  4. V. Sanz-Nebot
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  5. J. Barbosa
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  6. M. Valcárcel
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Corresponding author

Correspondence to M. Valcárcel.

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Almeda, S., Arce, L., Benavente, F. et al. Comparison of off- and in-line solid-phase extraction for enhancing sensitivity in capillary electrophoresis using ochratoxin as a model compound. Anal Bioanal Chem 394, 609–615 (2009). https://doi.org/10.1007/s00216-009-2696-x

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  • DOI: https://doi.org/10.1007/s00216-009-2696-x

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