Abstract
Reactive oxygen species (ROS) and reactive nitrogen species (RNS) play a crucial role in chemical signaling processes of biological cells. Electrochemistry is one of the rare methods able to directly detect these species. ROS and RNS can be monitored in the local microenvironment of cells in real time at the site where the actual signaling takes place. This review presents recent advances made with amperometric electrochemical techniques. Existing challenges for the quantification of ROS and RNS in biological systems are discussed to promote the development of innovative and reliable cell-based assays.
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Abbreviations
- HEPES:
-
4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid
- NOS:
-
nitric oxide synthases
- RNS:
-
reactive nitrogen species
- ROS:
-
reactive oxygen species
- SECM:
-
scanning electrochemical microscope
References
Thannickal VJ, Fanburg BL (2000) Am J Physiol Lung Cell Mol Physiol 279:L1005
Landar A, Darley-Usmar VM (2003) Amino Acids 25:313
Pryor WA, Houk KN, Foote CS, Fukuto JM, Ignarro LJ et al (2006) Am J Physiol Regul Integr Comp Physiol 291:R491
Yao DD, Vlessidis AG, Evmiridis NP (2004) Microchim Acta 147:1
Tarpey MM, Fridovich I (2001) Circ Res 89:224
Tarpey MM, Wink DA, Grisham MB (2004) Am J Physiol Regul Integr Comp Physiol 286:R431
Christodoulou D, Kudo S, Cook JA, Krishna MC, Miles A et al (1996) In: Methods in enzymology. Academic, San Diego, pp 69
Bedioui F, Villeneuve N (2003) Electroanalysis 15:5
Taha ZH (2003) Talanta 61:3
Wilson GS, Gifford R (2005) Biosens Bioelectron 20:2388
Wadsworth R, Stankevicius E, Simonsen U (2006) J Vasc Res 43:70
Yuasa M, Oyaizu K (2005) Curr Org Chem 9:1685
Miyasaka T, Endo K, Mochizuki S, Sakai K (2006) Sens Lett 4:144
Amatore C, Arbault S, Bouton C, Coffi K, Drapier JC et al (2006) Chembiochem 7:653
Archer S (1993) FASEB J 7:349
Henry Y, Lepoivre M, Drapier JC, Ducrocq C, Boucher JL et al (1993) FASEB J 7:1124
Guissani A, Henry YA (1997) J Mol Struct 404:257
Komarov AM (2002) In: Methods in enzymology. Academic, San Diego, p 66
Yoshimura T, Kotake Y (2004) Antioxid Redox Signal 6:639
Bartosz G (2006) Clin Chim Acta 368:53
Pustelny K, Bielanska J, Plonka PM, Rosen GM, Elas M (2007) Nitric Oxide Biol Chem 16:202
Miles AM, Wink DA, Cook JC, Grisham MB (1996) In: Nitric oxide. Academic, San Diego, p 105
Kojima H, Nakatsubo N, Kikuchi K, Kawahara S, Kirino Y et al (1998) Anal Chem 70:2446
Itoh Y, Ma FH, Hoshi H, Oka M, Noda K et al (2000) Anal Biochem 287:203
Munzel T, Afanas'ev IB, Kleschyov AL, Harrison DG (2002) Arterioscler Thromb Vasc Biol 22:1761
Gomes A, Fernandes E, Lima J (2006) J Fluoresc 16:119
Soh N (2006) Anal Bioanal Chem 386:532
Roychowdhury S, Luthe A, Keilhoff G, Wolf G, Horn TFW (2002) Glia S78
Zhang X, Kim WS, Hatcher N, Potgieter K, Moroz LL et al (2002) J Biol Chem 277:48472
Roychowdhury S, Luthe A, Keilhoff G, Wolf G, Horn TFW (2002) Glia 38:103
Tsikas D (2005) Free Radic Res 39:797
Kiechle FL, Malinski T (1996) Ann Clin Lab Sci 26:501
Wunder F, Stasch JP, Hutter J, Alonso-Alija C, Huser J et al (2005) Anal Biochem 339:104
Daiber A, Bachschmid M, Kavakli C, Frein D, Wendt M et al (2003) Nitric Oxide Biol Chem 9:44
Wightman RM (2006) Science 311:1570
Schulte A, Schuhmann W (2007) Angew Chem Int Ed 46:8760
Wilson GS, Johnson MA (2008) Chem Rev 108:2462
Adams KL, Puchades M, Ewing AG (2008) Annu Rev Anal Chem 1:329
Amatore C, Arbault S, Guille M, Lemaitre F (2008) Chem Rev 108:2585
Lowenstein CJ, Dinerman JL, Snyder SH (1994) Ann Intern Med 120:227
Anggard E (1994) Lancet 343:1199
Ignarro LJ (1999) Angew Chem Int Ed 38:1882
Ignarro LJ (2002) J Card Surg 17:301
Ignarro LJ (2002) J Physiol Pharmacol 53:503
Llorens S, Jordan J, Nava E (2002) J Physiol Biochem 58:179
Saraiva RM, Hare J (2006) Curr Opin Cardiol 21:221
Moncada S, Higgs EA (2006) Br J Pharmacol 147:S193
Bruhwyler J, Chleide E, Liegeois JF, Carreer F (1993) Neurosci Biobehav Rev 17:373
Garthwaite J (1993) Semin Neurosci 5:171
Szabo C (1996) Brain Res Bull 41:131
Prast H, Philippu A (2001) Prog Neurobiol 64:51
Talavera-Cuevas E, Condes-Lara M, Martinez-Lorenzana G (2003) Salud Ment 26:42
Rosen GM, Pou S, Ramos CL, Cohen MS, Britigan BE (1995) FASEB J 9:200
MacMicking J, Xie QW, Nathan C (1997) Annu Rev Immunol 15:323
Zaki MH, Akuta T, Akaike T (2005) J Pharmacol Sci 98:117
Wu GY, Morris SM (1998) Biochem J 336:1
Alderton WK, Cooper CE, Knowles RG (2001) Biochem J 357:593
Geller DA, Billiar TR (1998) Cancer Metastasis Rev 17:7
Pfeiffer S, Mayer B, Hemmens B (1999) Angew Chem Int Ed 38:1715
Ghafourifar P, Cadenas E (2005) Trends Pharmacol Sci 26:190
Lancaster JR (1997) Nitric Oxide Biol Chem 1:18
Brovkovych V, Stolarczyk E, Oman J, Tomboulian P, Malinski T (1999) J Pharm Biomed Anal 19:135
Chen B, Deen WM (2001) Chem Res Toxicol 14:135
Lee Y, Yang J, Rudich SM, Schreiner RJ, Meyerhoff ME (2004) Anal Chem 76:545
Isik S, Etienne M, Oni J, Blochl A, Reiter S et al (2004) Anal Chem 76:6389
Isik S, Schuhmann W (2006) Angew Chem Int Ed 45:7451
Brown GC, Borutaite V (2006) Biochem Soc Trans 34:953
Szabo C, Ischiropoulos H, Radi R (2007) Nat Rev Drug Discov 6:662
Halliwell B (2006) Plant Physiol 141:312
Rubbo H, Tarpey M, Freeman BA (1995) In: Free radicals and oxidative stress: environment, drugs and food additives, Portland, London, p 33
Sun AY, Chen YM (1998) J Biomed Sci 5:401
Floyd RA (1999) Proc Soc Exp Biol Med 222:236
Kamata H, Hirata H (1999) Cell Signal 11:1
Cai H, Harrison DG (2000) Circ Res 87:840
Calabrese V, Bates TE, Stella AMG (2000) Neurochem Res 25:1315
Radi R, Cassina A, Hodara R (2002) Biol Chem 383:401
Tieu K, Ischiropoulos H, Przedborski S (2003) IUBMB Life 55:329
Poli G, Leonarduzzi G, Biasi F, Chiarpotto E (2004) Curr Med Chem 11:1163
Poon HF, Calabrese V, Scapagnini G, Butterfield DA (2004) Clin Geriatr Med 20:329
Keynes RG, Garthwaite J (2004) Curr Mol Med 4:179
Dalle-Donne I, Scaloni A, Giustarini D, Cavarra E, Tell G et al (2005) Mass Spectrom Rev 24:55
Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M (2006) Chem Biol Interact 160:1
Ridnour LA, Thomas DD, Mancardi D, Espey MG, Miranda KM et al (2004) Biol Chem 385:1
Rassaf T, Feelisch M, Kelm M (2004) Free Radic Biol Med 36:413
Mancardi D, Ridnour LA, Thomas DD, Katori T, Tocchetti CG et al (2004) Curr Mol Med 4:723
Bogdan C (2001) Nat Immunol 2:907
Pontie M, Bedioui F (1999) Analusis 27:564
Borgmann S, Hartwich G, Schulte A, Schuhmann W (2006) In: Palecek E, Scheller F, Wang J (eds) Electrochemistry of nucleic acids and proteins. Towards electrochemical sensors for genomics and proteomics. Elsevier, Amsterdam, p 599
Vessman J, Stefan RI, Van Staden JF, Danzer K, Lindner W et al (2001) Pure Appl Chem 73:1381
Lee KY, Amatore C, Kochi JK (1991) J Phys Chem 95:1285
Koppenol WH, Moreno JJ, Pryor WA, Ischiropoulos H, Beckman JS (1992) Chem Res Toxicol 5:834–842
Koppenol WH (1996) In: Nitric oxide, part A—sources and detection of NO; NO synthase. Academic, San Diego, pp 7
Koppenol WH (1998) Free Radic Biol Med 25:385
Bedioui F, Trevin S (1998) Biosens Bioelectron 13:227
Lide DR (ed) (2004–2005) Handbook of chemistry and physics, 85th edn. CRC, Boca Raton
Küster FW, Thiel A (1985) Rechentafeln für die chemische Analytik, bearbeitet von A. Ruland. de Gruyter, Berlin, pp 103
Phillips PEM, Wightman RM (2003) Trends Anal Chem 22:509
Keynes RG, Griffiths C, Garthwaite J (2003) Biochem J 369:399
Johnson CD, Paul DW (2005) Sens Actuators B Chem 105:322
Park J, Kim CS, Kim Y (2005) Sens Actuators B Chem 108:633
Lee Y, Oh BK, Meyerhoff ME (2004) Anal Chem 76:536
Borgmann S, Radtke I, Erichsen T, Blochl A, Heumann R et al (2006) Chembiochem 7:662
Erichsen T, Reiter S, Ryabova V, Bonsen EM, Schuhmann W et al (2005) Rev Sci Instrum 76:062204
Bard AJ, Fan FRF, Kwak J, Lev O (1989) Anal Chem 61:132
Mirkin MV, Horrocks BR (2000) Anal Chim Acta 406:119
Barker AL, Gonsalves M, Macpherson JV, Slevin CJ, Unwin PR (1999) Anal Chim Acta 385:223
Pust SE, Maier W, Wittstock G (2008) Z Phys Chem 222:1463–1517
Pailleret A, Oni J, Reiter S, Isik S, Etienne M et al (2003) Electrochem Commun 5:847
Kwak J, Bard AJ (1989) Anal Chem 61:1221
Engstrom RC, Pharr CM (1989) Anal Chem 61:1099–1104
Whitesides GM (2006) Nature 442:368
Fox MB, Esveld DC, Valero A, Luttge R, Mastwijk HC et al (2006) Anal Bioanal Chem 385:474
Chen CS, Jiang XY, Whitesides GM (2005) MRS Bull 30:194
Yi CQ, Li CW, Ji SL, Yang MS (2006) Anal Chim Acta 560:1
Delamarche E, Juncker D, Schmid H (2005) Adv Mater 17:2911
El-Ali J, Sorger PK, Jensen KF (2006) Nature 442:403
Wartelle C, Schuhmann W, Blochl A, Bedioui F (2005) Electrochim Acta 50:4988
Chang SC, Pereira-Rodrigues N, Henderson JR, Cole A, Bedioui F et al (2005) Biosens Bioelectron 21:917
Castillo J, Isik S, Blochl A, Pereira-Rodrigues N, Bedioui F et al (2005) Biosens Bioelectron 20:1559
Oni J, Pailleret A, Isik S, Diab N, Radtke I et al (2004) Anal Bioanal Chem 378:1594
Brischwein M, Grothe H, Otto AM, Ressler J, Lob V et al (2005) Chem Ing Tech 77:1955
Castillo J, Blochl A, Dennison S, Schuhmann W, Csoregi E (2005) Biosens Bioelectron 20:2116
Otto AM, Brischwein M, Niendorf A, Henning T, Motrescu E et al (2003) Cancer Detect Prev 27:291
Andreescu S, Sadik OA, McGee DW (2004) Anal Chem 76:2321
Andreescu S, Sadik OA (2005) Methods 37:84
Karasinski J, Andreescu S, Sadik OA, Lavine B, Vora MN (2005) Anal Chem 77:7941
Zhang B, Adams KL, Luber SJ, Eves DJ, Heien ML et al (2008) Anal Chem 80:1394
Krylov AV, Beissenhirtz M, Adamzig H, Scheller FW, Lisdat F (2004) Anal Bioanal Chem 378:1327
Krylov AV, Adamzig H, Walter AD, Lochel B, Kurth E et al (2006) Sens Actuators B Chem 119:118
Krylov AV, Sczech R, Lisdat F (2007) Analyst 132:135
Lvovich V, Scheeline A (1997) Anal Chem 69:454
Zen JM, Kumar AS, Wang HF (2000) Analyst 125:2169
Lei JP, Ju HX, Ikeda O (2004) J Electroanal Chem 567:331
Manning P, McNeil CJ, Cooper JM, Hillhouse EW (1998) Free Radic Biol Med 24:1304
Manning P, Cookson MR, Eggett CJ, Tolias CM, Read SJ et al (2000) Analusis 28:493
Kubant R, Malinski C, Burewicz A, Malinski T (2006) Electroanalysis 18:410
Patel BA, Arundell M, Quek RGW, Harvey SLR, Ellis IR et al (2008) Anal Bioanal Chem 390:1379
Amatore C, Arbault S, Bouton C, Drapier JC, Ghandour H et al (2008) Chembiochem 9:1472
Griess P (1879) Ber Dtsch Chem Ges 12:426
Schulz K, Kerber S, Kelm M (1999) Nitric Oxide Biol Chem 3:225
Acknowledgements
The author greatly appreciates the generous support from C.M. Niemeyer (Chemical Biology, Technische Universität Dortmund) and ISAS—Institute for Analytical Sciences, Dortmund. The many valuable discussions with current and former colleagues are greatly acknowledged. The author is grateful to L. Koch for help with some of the graphics.
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Borgmann, S. Electrochemical quantification of reactive oxygen and nitrogen: challenges and opportunities. Anal Bioanal Chem 394, 95–105 (2009). https://doi.org/10.1007/s00216-009-2692-1
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DOI: https://doi.org/10.1007/s00216-009-2692-1