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Simultaneous quantification of nicotine, opioids, cocaine, and metabolites in human fetal postmortem brain by liquid chromatography tandem mass spectrometry

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Abstract

A validated method for simultaneous LCMSMS quantification of nicotine, cocaine, 6-acetylmorphine (6AM), codeine, and metabolites in 100 mg fetal human brain was developed and validated. After homogenization and solid-phase extraction, analytes were resolved on a Hydro-RP analytical column with gradient elution. Empirically determined linearity was from 5–5,000 pg/mg for cocaine and benzoylecgonine (BE), 25–5,000 pg/mg for cotinine, ecgonine methyl ester (EME) and 6AM, 50-5000 pg/mg for trans-3-hydroxycotinine (OH-cotinine) and codeine, and 250–5,000 pg/mg for nicotine. Potential endogenous and exogenous interferences were resolved. Intra- and inter-assay analytical recoveries were ≥92%, intra- and inter-day and total assay imprecision were ≤14% RSD and extraction efficiencies were ≥67.2% with ≤83% matrix effect. Method applicability was demonstrated with a postmortem fetal brain containing 40 pg/mg cotinine, 65 pg/mg OH-cotinine, 13 pg/mg cocaine, 34 pg/mg EME, and 525 pg/mg BE. This validated method is useful for determination of nicotine, opioid, and cocaine biomarkers in brain.

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Acknowledgments

The authors are indebted to Dr. Ronald Zielke, Mr. Robert Vigoritto, Mr. Robert Johnson of the NICHD Brain and Tissue Bank for Development Disorders. Also, the authors are thankful to Thomas Hyde, MD, PhD, Dr. Joel Kleinman, PhD, and Elin Lehrmann, PhD. Funding was from the National Institutes of Health, Intramural Research Programs, National Institute on Drug Abuse and National Institute of Mental Health.

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Authors and Affiliations

  1. Chemistry and Drug Metabolism, Intramural Research Program, National Institute on Drug Abuse, Biomedical Research Center, 251 Bayview Boulevard Suite 5A721, Baltimore, MD, 21224, USA

    Diaa M. Shakleya & Marilyn A. Huestis

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  1. Diaa M. Shakleya
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  2. Marilyn A. Huestis
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Correspondence to Marilyn A. Huestis.

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Shakleya, D.M., Huestis, M.A. Simultaneous quantification of nicotine, opioids, cocaine, and metabolites in human fetal postmortem brain by liquid chromatography tandem mass spectrometry. Anal Bioanal Chem 393, 1957–1965 (2009). https://doi.org/10.1007/s00216-009-2661-8

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  • DOI: https://doi.org/10.1007/s00216-009-2661-8

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