Monitoring of cell cultures with LTCC microelectrode array

Abstract

Monitoring of cell cultures in microbioreactors is a crucial task in cell bioassays and toxicological tests. In this work a novel tool based on a miniaturized sensor array fabricated using low-temperature cofired ceramics (LTCC) technology is presented. The developed device is applied to the monitoring of cell-culture media change, detection of the growth of various species, and in toxicological studies performed with the use of cells. Noninvasive monitoring performed with the LTCC microelectrode array can be applied for future cell-engineering purposes.

Microelectrode array for monitoring of cell cultures

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Acknowledgment

This work was financially supported by the Ministry of Science and Higher Education, project no. N204 106 31/2448 (2006–2008). The authors thank Mr Piotr Żbikowski for assistance in LTCC structure fabrication. Patrycja Ciosek wishes to thank The Foundation for Polish Science for financial support.

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Correspondence to P. Ciosek.

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Ciosek, P., Zawadzki, K., Łopacińska, J. et al. Monitoring of cell cultures with LTCC microelectrode array. Anal Bioanal Chem 393, 2029–2038 (2009). https://doi.org/10.1007/s00216-009-2651-x

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Keywords

  • Sensor array
  • Cell cultures
  • LTCC
  • Ion-selective electrodes