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Optical diagnosis of peritoneal metastases by infrared microscopic imaging

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Abstract

Fourier transform infrared (FTIR) spectroscopy is nowadays widely accepted as a technique with high potential for diagnosis of cancerous tissues. This study presents an example of the investigation of peritoneal metastases by FTIR microimaging. Peritoneal malignancies are generally secondary localizations of primary visceral cancers such as ovarian, stomach or colon cancers. By analysing simultaneously both formalin-fixed paraffin-embedded and frozen specimens, we examined malignant and non-malignant (i.e. fibrotic and cicatricial) peritoneal lesions. Paraffin-embedded tissues were analysed without any previous dewaxing. Multivariate statistical approaches, based on the classification of infrared data by hierarchical cluster analysis, allowed the discrimination of these various samples. Microimaging also permits the revelation of the heterogeneity of the tissue: it was possible to localize precisely the cancerous areas, and to distinguish, on the basis of their spectral signatures, the peritumoral neighbouring connective tissue close to the carcinomatous areas from the connective tissue distant from the cancerous areas. These spectral differences could be useful as complementary information to study molecular changes associated with the malignancy.

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Abbreviations

FFPE:

formalin-fixed paraffin-embedded

FTIR:

Fourier transform infrared

HCA:

hierarchical cluster analysis

HE:

haematoxylin–eosin

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Acknowledgements

The authors would like to thank Paul Dumas for processing the data using Cytospec and the staff of the Laboratoire d'Anatomie et Cytologie Pathologiques of Reims University Hospital for their collaboration in this work and in particular Edith Pierson for her assistance with sample preparation.

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Authors and Affiliations

  1. Unité MéDIAN, CNRS UMR 6237 MEDyC, IFR53, UFR Pharmacie, Université de Reims Champagne-Ardenne, 51 rue Cognacq Jay, 51096, Reims Cedex, France

    Valérie Untereiner, Olivier Piot, Marie-Danielle Diebold, Olivier Bouché & Michel Manfait

  2. Laboratoire d’Anatomie et Cytologie Pathologiques, CHU Robert Debré, Avenue du Général Koenig, 51092, Reims Cedex, France

    Marie-Danielle Diebold

  3. Service d’Hépato-Gastroentérologie, CHU Robert Debré, Avenue du Général Koenig, 51092, Reims Cedex, France

    Olivier Bouché & Elodie Scaglia

Authors
  1. Valérie Untereiner
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  2. Olivier Piot
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  3. Marie-Danielle Diebold
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  4. Olivier Bouché
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  6. Michel Manfait
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Correspondence to Olivier Piot.

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Untereiner, V., Piot, O., Diebold, MD. et al. Optical diagnosis of peritoneal metastases by infrared microscopic imaging. Anal Bioanal Chem 393, 1619–1627 (2009). https://doi.org/10.1007/s00216-009-2630-2

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  • DOI: https://doi.org/10.1007/s00216-009-2630-2

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