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Plasma fingerprinting with GC-MS in acute coronary syndrome

Analytical and Bioanalytical Chemistry volume 394, pages 1517–1524 (2009)Cite this article

Abstract

New biomarkers of cardiovascular disease are needed to augment the information obtained from traditional indicators and to illuminate disease mechanisms. One of the approaches used in metabolomics/metabonomics for that purpose is metabolic fingerprinting aiming to profile large numbers of chemically diverse metabolites in an essentially nonselective way. In this study, gas chromatography-mass spectrometry was employed to evaluate the major metabolic changes in low molecular weight plasma metabolites of patients with acute coronary syndrome (n = 9) and with stable atherosclerosis (n = 10) vs healthy subjects without significant differences in age and sex (n = 10). Reproducible differences between cases and controls were obtained with pattern recognition techniques, and metabolites accounting for higher weight in the classification have been identified through their mass spectra. On this basis, it seems inherently plausible that even a simple metabolite profile might be able to offer improved clinical diagnosis and prognosis, but in addition, specific markers are being identified.

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References

  1. Wang TJ, Gona P, Larson MG, Tofler GH, Levy D, Newton-Cheh C, Jacques PF, Rifai N, Selhub J, Robins SJ, Benjamin EJ, D’Agostino RB, Vasan RS (2006) N Engl J Med 355:2631–2639

    Article  CAS  Google Scholar 

  2. Gerszten RE, Wang TJ (2008) Nature 451:949–952

    Article  CAS  Google Scholar 

  3. Arab S, Gramolini AO, Ping P, Kislinger T, Stanley B, van Eyk J, Ouzounian M, MacLennan DH, Emili A, Liu PP (2006) J Am Coll Cardiol 48:1733–1741

    Article  CAS  Google Scholar 

  4. Grainger DJ (2006) Heart Metab 32:22–25

    Google Scholar 

  5. Vasan RS (2006) Circulation 113:2335–2362

    Article  Google Scholar 

  6. Brindle JT, Nicholson JK, Schofield PM, Grainger DJ, Holmes E (2003) Analyst 128:32–36

    Article  CAS  Google Scholar 

  7. Brindle JT, Antti H, Holmes E, Tranter G, Nicholson JK, Bethell HW, Clarke S, Schofield PM, McKilligin E, Mosedale DE, Grainger DJ (2002) Nat Med 8(12):1439–1444

    Article  CAS  Google Scholar 

  8. Kirschenlohr HL, Griffin JL, Clarke SC, Rhydwen R, Grace AA, Schofield PM, Brindle KM, Metcalfe JC (2006) Nat Med 12(6):705–710

    Article  CAS  Google Scholar 

  9. Sabatine MS, Liu E, Morrow DA, Heller E, McCarroll R, Wiegand R, Berriz GF, Roth FP, Gerszten RE (2005) Circulation 112:3868–3875

    Article  CAS  Google Scholar 

  10. Jiye A, Trygg J, Gullberg J, Johansson AI, Jonsson P, Antti H, Marklund SL, Moritz T (2005) Anal Chem 77:8086–8094

    Article  Google Scholar 

  11. Daykin CA, Foxall PJD, Connor SC, Lindon JC, Nicholson JK (2002) Anal Biochem 304(2):220–230

    Article  CAS  Google Scholar 

  12. Nielsen NP, Carstensen JM, Smedsgaard J (1998) J Chromatogr A 805:17–35

    Article  CAS  Google Scholar 

  13. Angulo S, García-Pérez I, Legido-Quigley C, Barbas C (2008) Electrophoresis (in press)

  14. Barbas C, Vallejo M, Garcia A, Barlow D, Hanna-Brown A (2008) J Pharm Biomed Anal 47:388–398

    Article  CAS  Google Scholar 

  15. Vallejo M, Angulo S, Garcia-Martinez D, Garcia A, Barbas C (2008) J Chromatogr A 1187:267–274

    Article  CAS  Google Scholar 

  16. Sabatine MS, Liu E, Morrow DA, Heller E, McCarroll R, Wiegand R, Berriz GF, Roth FP, Gerszten RE (2005) Circulation 112:3868–3875

    Article  CAS  Google Scholar 

  17. Jormsjo S, Wuttge DM, Sirsjo A, Whatling C, Hamsten A, Stemme S, Eriksson P (2002) AM J Pathol 161:939–945

    Google Scholar 

  18. Berg RA (1982) Methods Enzymol 82:372–378

    Article  CAS  Google Scholar 

  19. Trieu VN, Zioncheck TF, Lawn RM, McConathy WJ (1991) J Biol Chem 266:5480–5485

    CAS  Google Scholar 

  20. Rath M (1992) J Orthomol Med 7:153–162

    Google Scholar 

  21. Bernstein AM, Willcox BJ, Tamaki H, Kunishima N, Suzuki M, Willcox DC, Yoo JS, Perls TT (2004) J Gerontol (A Biol Sci Med Sci) 59:1195–1199

    Google Scholar 

  22. Davies MJ (1996) Circulation 94(8):2013–2020

    CAS  Google Scholar 

  23. Shah PK, Falk E, Badimon JJ, Fernandez-Ortiz A, Mailhac A, Villareal-Levy G, Fallon JT, Regnstrom J, Fuster V (1995) Circulation 92(6):1565–1569

    CAS  Google Scholar 

  24. Toschi V, Gallo R, Lettino M, Fallon JT, Gertz SD, Fernández-Ortiz A, Chesebro JH, Badimon L, Nemerson Y, Fuster V, Badimon JJ (1997) Circulation 95(3):594–599

    CAS  Google Scholar 

Download references

Acknowledgments

This work has been supported by the Comunidad Autónoma de Madrid (S2006/GEN-0247), Spanish Ministery of Sciences and Technology CTQ2008-03779, Fundación Ramón Areces, FIS (PI050451), and Sociedad Española de Arteriosclerosis.

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Affiliations

  1. Pharmacy Faculty, San Pablo-CEU University Madrid, Campus Montepríncipe, Boadilla del Monte, 28668, Madrid, Spain

    M. Vallejo, A. García, D. García-Martínez, S. Angulo & C. Barbas

  2. Cardiology Service, Fundación Jiménez Díaz, Madrid, Spain

    J. Tuñón & P. Almeida

  3. Vascular Research Laboratory, Fundación Jiménez Díaz, Madrid, Spain

    J.L. Martin-Ventura, L. M. Blanco-Colio & J. Egido

  4. Autónoma University, Madrid, Spain

    J. Tuñón, J.L. Martin-Ventura, L. M. Blanco-Colio & J. Egido

Authors
  1. M. Vallejo
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  2. A. García
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  3. J. Tuñón
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  4. D. García-Martínez
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  5. S. Angulo
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  6. J.L. Martin-Ventura
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  7. L. M. Blanco-Colio
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  8. P. Almeida
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  9. J. Egido
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  10. C. Barbas
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Corresponding author

Correspondence to C. Barbas.

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Vallejo, M., García, A., Tuñón, J. et al. Plasma fingerprinting with GC-MS in acute coronary syndrome. Anal Bioanal Chem 394, 1517–1524 (2009). https://doi.org/10.1007/s00216-009-2610-6

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  • DOI: https://doi.org/10.1007/s00216-009-2610-6

Keywords

  • Acute coronary syndrome
  • Atherosclerosis
  • Metabonomics
  • Metabolomics
  • Pattern recognition
  • Separation techniques
  • Stroke