Abstract
This paper reports on a rapid and sensitive method for the simultaneous determination of ascorbic acid (H2A), dehydroascorbic acid (DHA), and total vitamin C by electrochemiluminescence (ECL) using a thin-layer electrochemical cell. Significant ECL signals can be generated by the anodic oxidation of Ru(bpy)3 2+ in the presence of H2A or DHA in pH 8.8 phosphate buffer solution. Because of the extremely small dead volume of the thin-layer cell (approximately 1.5 μL), almost all amount of H2A is assumed to be completely oxidized to DHA with a short pre-electrolysis step. As a result, it is possible to determine the reductive vitamin C (H2A) by square wave voltammetry before the pre-electrolysis step, while total vitamin C (sum of H2A and DHA) is able to be determined at a subsequent ECL step. The method was employed for the determination of vitamin C in commercial beverages with the analytical results in good agreement with the certified values.
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Acknowledgements
This work was partially supported by a Grant-in-Aid (no. 18655030) for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology in Japan.
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Takahashi, F., Jin, J. Rapid determination of ascorbic acid, dehydroascorbic acid, and total vitamin C by electrochemiluminescence with a thin-layer electrochemical cell. Anal Bioanal Chem 393, 1669–1675 (2009). https://doi.org/10.1007/s00216-008-2597-4
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DOI: https://doi.org/10.1007/s00216-008-2597-4