Analytical and Bioanalytical Chemistry

, Volume 393, Issue 4, pp 1357–1360 | Cite as

High-throughput non-destructive nuclear magnetic resonance method to measure intramuscular fat content in beef

  • Cátia Crispilho Corrêa
  • Lucimara Aparecida Forato
  • Luiz Alberto Colnago
Technical Note


High intake of saturated fat from meats has been associated with cardiovascular disease, cancer, diabetes, and others diseases. In this paper, we are introducing a simple, high-throughput, and non-destructive low-resolution nuclear magnetic resonance method that has the potential to analyze the intramuscular fat content (IMF) in more than 1,000 beef portions per hour. The results can be used in nutritional fact labels, replacing the currently used average value. The method is based on longitudinal (T 1) and transverse (T 2) relaxation time information obtained by a continuous wave-free precession (CWFP) sequence. CWFP yields a higher correlation coefficient (r = 0.9) than the conventional Carr-Purcell-Meiboom-Gill (CPMG) method (r = −0.25) for IMF in beef and is just as fast and a simpler pulse sequence than CPMG. The method can also be applied to other meat products.


Low-resolution NMR CPMG CWFP Intramuscular fat analysis 



We wish to thank FAPESP, CNPq, and FINEP/RBT for financial support.


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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Cátia Crispilho Corrêa
    • 1
    • 2
  • Lucimara Aparecida Forato
    • 1
  • Luiz Alberto Colnago
    • 1
  1. 1.Embrapa Instrumentação AgropecuáriaSão CarlosBrazil
  2. 2.Instituto de Química de São CarlosUniversidade de São PauloSão CarlosBrazil

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