Analytical and Bioanalytical Chemistry

, Volume 393, Issue 4, pp 1157–1163 | Cite as

Label-free detection of cancer biomarker candidates using surface plasmon resonance imaging

  • Jon Ladd
  • Allen D. Taylor
  • Marek Piliarik
  • Jiri Homola
  • Shaoyi Jiang
Original Paper

Abstract

In this work, we present an antibody array for the detection of cancer biomarker candidates by a surface plasmon resonance (SPR) imaging sensor with polarization contrast. Responses from the SPR imaging sensor are shown to be similar to those from a conventional spectroscopy-based SPR sensor. Antibodies are spotted onto a self-assembled monolayer (SAM) composed of oligo(ethylene glycol) (OEG)-containing alkanethiol chains. Detection of two cancer biomarker candidates, activated leukocyte cell adhesion molecule/CD 166 (ALCAM) and transgelin-2 (TAGLN2), is demonstrated. Limits of detection for ALCAM and TAGLN2 are established at 6 ng/mL and 3 ng/mL, respectively, in buffer. No cross-reactivity is observed between immobilized antibodies and nonspecific antigen. Biomarker candidates are also detected in a 10% human serum solution.

Keywords

Surface plasmon resonance imaging Protein array Cancer biomarkers 

Supplementary material

216_2008_2448_MOESM1_ESM.pdf (126 kb)
ESM 1(PDF 126 KB).

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Jon Ladd
    • 1
  • Allen D. Taylor
    • 1
  • Marek Piliarik
    • 2
  • Jiri Homola
    • 2
  • Shaoyi Jiang
    • 1
  1. 1.Department of Chemical EngineeringUniversity of WashingtonSeattleUSA
  2. 2.Institute of Photonics and Electronics of the Academy of Sciences of the Czech RepublicPragueCzech Republic

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