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Site-specific binding of chelerythrine and sanguinarine to single pyrimidine bulges in hairpin DNA

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Abstract

Spectrofluorometric titration, electrospray ionization time-of-flight mass spectrometric and UV melting methods were employed to study the binding of chelerythrine and sanguinarine to bulged DNA. The results showed that both alkaloids bind specifically to single pyrimidine (C, T) bulge sites. The ability of sanguinarine to bind to both regular and bulged hairpins was found to be stronger than that of chelerythrine, but the binding selectivity of chelerythrine toward single-base bulges was much larger than that of sanguinarine.

Association constants for chelerythrine and sanguinarine toward regular and single-base bulged hairpins obtained from fluorometric analysis

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Acknowledgements

This work was financially supported by the Research Grants Council, the University Grants Committee of Hong Kong (HKBU2455/06M), and the Faculty Research Grant of Hong Kong Baptist University.

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Authors and Affiliations

  1. School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong

    Li-Ping Bai, Zhong-Zhen Zhao & Zhi-Hong Jiang

  2. Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong

    Zongwei Cai

  3. Department of Regulatory Bioorganic Chemistry, The Institute of Scientific and Industrial Research (SANKEN), Osaka University, 8–1 Mihogaoka, Ibaraki, 567–0047, Japan

    Kazuhiko Nakatani

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  1. Li-Ping Bai
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  2. Zongwei Cai
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  3. Zhong-Zhen Zhao
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Correspondence to Zhi-Hong Jiang.

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Bai, LP., Cai, Z., Zhao, ZZ. et al. Site-specific binding of chelerythrine and sanguinarine to single pyrimidine bulges in hairpin DNA. Anal Bioanal Chem 392, 709–716 (2008). https://doi.org/10.1007/s00216-008-2302-7

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  • DOI: https://doi.org/10.1007/s00216-008-2302-7

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