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Simultaneous determination of hydrochlorothiazide and losartan potassium in tablets by high-performance low-pressure chromatography using a multi-syringe burette coupled to a monolithic column

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Abstract

This contribution describes use of a separation method based on on-line coupling of a multisyringe flow system with a chromatographic monolithic column for simultaneous determination of hydrochlorothiazide and losartan potassium in tablets. The system comprised a multisyringe module, three low-pressure solenoid valves, a monolithic C18 column (25 mm × 4.6 mm i.d.), and a diode-array detector. The mobile phase was 10 mmol L−1 potassium dihydrogen phosphate (pH 3.1)-acetonitrile-methanol (65:33:2 v/v/v) at a flow rate 0.8 mL min−1. UV detection was carried out at 226 nm. The multi-syringe chromatographic (MSC) method with UV spectrophotometric detection was optimized and validated. Results from validation were very good. The analysis time was about 400 s. The method was found to be applicable to routine analysis of both compounds in tablets. The coupling of the monolithic columns with a multi-syringe flow-injection analysis manifold provides an excellent and inexpensive tool to solve the separation problems without use of HPLC instrumentation.

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Acknowledgments

Thanks are due to the Spanish Ministry of Science and Technology for supporting the projects CTQ 2007–64331 and CTQ 2004–03256. María Antonieta Obando thanks FONACIT for the award of a sabbatical grant.

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Authors and Affiliations

  1. Analysis and Quality Department, Faculty of Pharmacy, Los Andes University, 5101, Mérida, Venezuela

    María Antonieta Obando

  2. Department of Chemistry, Faculty of Sciences, University of the Balearic Islands, 07122, Palma de Mallorca, Spain

    José Manuel Estela & Víctor Cerdà

Authors
  1. María Antonieta Obando
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  2. José Manuel Estela
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  3. Víctor Cerdà
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Corresponding author

Correspondence to Víctor Cerdà.

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Obando, M.A., Estela, J.M. & Cerdà, V. Simultaneous determination of hydrochlorothiazide and losartan potassium in tablets by high-performance low-pressure chromatography using a multi-syringe burette coupled to a monolithic column. Anal Bioanal Chem 391, 2349–2356 (2008). https://doi.org/10.1007/s00216-008-2172-z

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  • DOI: https://doi.org/10.1007/s00216-008-2172-z

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