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Sensitive HPLC–fluorescence detection of morphine labeled with DIB-Cl in rat brain and blood microdialysates and its application to the preliminarily study of the pharmacokinetic interaction between morphine and diclofenac

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Abstract

A sensitive high-performance liquid chromatography (HPLC)–fluorescence method for determination of morphine (Mor) in rat brain and blood microdialysates was developed using 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride (DIB-Cl) as a label. Mor was labeled with DIB-Cl under mild reaction conditions (at room temperature for 10 min). The separation of DIB-Mor was carried out on an octadecylsilica (ODS) column with CH3CN/0.1 M acetate buffer (pH 5.4) within 14 min. The detection limits of Mor in brain and blood microdialysates at a signal-to-noise ratio of 3 were 0.4 and 0.6 ng mL−1, respectively. The proposed method was successfully applied to the preliminarily study of potential pharmacokinetic interaction between Mor and diclofenac.

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Acknowledgment

The authors gratefully acknowledge the financial support from Ministry of Health, Labor and Welfare of Japan.

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Authors and Affiliations

  1. Department of Clinical Pharmacy, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki, 852-8521, Japan

    Mitsuhiro Wada, Chiaki Yokota, Yasuhiro Ogata, Naotaka Kuroda & Kenichiro Nakashima

  2. Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan

    Hideyuki Yamada

Authors
  1. Mitsuhiro Wada
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  2. Chiaki Yokota
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  3. Yasuhiro Ogata
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  4. Naotaka Kuroda
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  5. Hideyuki Yamada
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  6. Kenichiro Nakashima
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Correspondence to Kenichiro Nakashima.

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Wada, M., Yokota, C., Ogata, Y. et al. Sensitive HPLC–fluorescence detection of morphine labeled with DIB-Cl in rat brain and blood microdialysates and its application to the preliminarily study of the pharmacokinetic interaction between morphine and diclofenac. Anal Bioanal Chem 391, 1057–1062 (2008). https://doi.org/10.1007/s00216-008-2070-4

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  • DOI: https://doi.org/10.1007/s00216-008-2070-4

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