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Identification of changes in Triticum durum L. leaf proteome in response to salt stress by two-dimensional electrophoresis and MALDI-TOF mass spectrometry

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Abstract

In order to understand the molecular basis of salt stress response, a proteomic approach, employing two-dimensional electrophoresis and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS), was used to identify proteins affected by salinity in wheat (Triticum durum ‘Ofanto’). Identification of proteins, whose levels were altered, was performed by comparing protein patterns of salt-treated and control plants. A set of control plants was grown without NaCl addition under the same conditions as the salt-treated plants. Proteins were extracted from the leaves of untreated and NaCl-treated plants, and resolved using 24-cm immobilized pH gradient strips with a pH 4–7 linear gradient in the first dimension and a 12.5% sodium dodecyl sulphate polyacrylamide gel electrophoresis in the second dimension; the gels were stained with Coomassie and image analysis was performed. Quantitative evaluation, statistical analyses and MALDI-TOF MS characterization of the resolved spots in treated and untreated samples enabled us to identify 38 proteins whose levels were altered in response to salt stress. In particular, ten proteins were downregulated and 28 were upregulated. A possible role of these proteins in response to salinity is discussed.

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Acknowledgement

This work was supported by the Italian Department of Agriculture Food and Forestry, in the framework of Triticum durum quality programme (FRUMISIS project).

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Authors and Affiliations

  1. Department of Chemistry, “Sapienza” University of Rome, Piazzale Aldo Moro 5, 00185, Rome, Italy

    Giuseppe Caruso, Chiara Cavaliere, Chiara Guarino, Riccardo Gubbiotti, Patrizia Foglia & Aldo Laganà

  2. Dipartimento di Chimica, “Sapienza” Università di Roma, Box no. 34, Roma 62, Piazzale Aldo Moro 5, 00185, Rome, Italy

    Aldo Laganà

Authors
  1. Giuseppe Caruso
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  2. Chiara Cavaliere
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  3. Chiara Guarino
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  4. Riccardo Gubbiotti
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  5. Patrizia Foglia
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  6. Aldo Laganà
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Correspondence to Aldo Laganà.

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Caruso, G., Cavaliere, C., Guarino, C. et al. Identification of changes in Triticum durum L. leaf proteome in response to salt stress by two-dimensional electrophoresis and MALDI-TOF mass spectrometry. Anal Bioanal Chem 391, 381–390 (2008). https://doi.org/10.1007/s00216-008-2008-x

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  • DOI: https://doi.org/10.1007/s00216-008-2008-x

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