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Antioxidative effect of Bacteroides thetaiotaomicron extracts: superoxide dismutase identification

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Abstract

Bacteroides thetaiotaomicron, a bowel anaerobic commensal, seems to release enzymes detoxifying reactive oxygen species according to our recent work. This opportunistic pathogen would be beneficial in the case of an inflammatory process. To explore its role after an oxidative or nutritive stress, six to seven separate experiments were performed. The bacteria were grown on media restricted in growth factors or supplemented with bile. Their viability was checked after surface protein extraction. The extracts underwent 2D electrophoresis. Gel images were statistically analysed to construct “master” gels. Proteins were identified (peptide-mass fingerprinting technique). The effect of each extract on superoxide anions was evaluated (spectrophotometric method). Superoxide dismutase was identified and a major superoxide anion inhibition was shown by extracts obtained after a nutritive and oxidative stress without significant bacterial death. So, a therapeutic antioxidant potential is firmly hoped for.

These intestinal commensal Gram negative anaerobic bacilli are able to release a high level of functional superoxide dismutase when grown in minimal medium.

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Acknowledgement

The authors thank D Huges for his helpful assistance.

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Authors and Affiliations

  1. Laboratoire de Bactériologie Clinique, Faculté de Pharmacie de Lille, Université de Lille 2, 3 rue du Pr Laguesse, BP 83, 59006, Lille cedex, France

    Anne-Cécile Hochart-Behra, Josette Behra-Miellet, Julie Sam & Luc Dubreuil

  2. Institut de Biologie de Lille, 1 rue du Pr Calmette, BP 447, 59021, Lille cedex, France

    Hervé Drobecq

  3. Laboratoire de Pharmacologie, Pharmacocinétique et Pharmacie Clinique, Faculté de Pharmacie de Lille, Université de Lille 2, 3 rue du Pr Laguesse, BP 83, 59006, Lille cedex, France

    Bernard Gressier, Michel Luyckx, Thierry Dine & Claude Brunet

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  1. Anne-Cécile Hochart-Behra
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  2. Josette Behra-Miellet
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  3. Julie Sam
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  5. Bernard Gressier
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  6. Michel Luyckx
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  7. Thierry Dine
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  9. Luc Dubreuil
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Correspondence to Josette Behra-Miellet.

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Hochart-Behra, AC., Behra-Miellet, J., Sam, J. et al. Antioxidative effect of Bacteroides thetaiotaomicron extracts: superoxide dismutase identification. Anal Bioanal Chem 391, 415–423 (2008). https://doi.org/10.1007/s00216-008-1993-0

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  • DOI: https://doi.org/10.1007/s00216-008-1993-0

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