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Determination of IGF-I in horse plasma by LC electrospray ionisation mass spectrometry

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Abstract

The insulin-like-growth factor (IGF-I) peptide is considered to be the main indirect marker for growth hormone administration (GH) in a horse. Further to a previous investigation on measurement of IGF-I in plasma samples by mass spectrometry, this study focuses on quantitative and qualitative analysis of intact IGF-I in horse plasma. First, protein-transposing software has been developed for IGF-I to facilitate its quantification by HPLC–electrospray–ion-trap mass spectrometry. Second, product-ion scan experiments on IGF-I have been conducted on standard samples, non-fortified equine plasma samples, fortified plasma samples, and equine GH post-administration samples. This “top-down” approach method enables characterisation of fragment ions corresponding to the carboxy terminal end, which can be useful for the confirmation of the presence of IGF-I in plasma samples.

Structure of IGF-I and amino acid sequences of IGF-I and R3 IGF-I. Deconvolution mass spectra of the IGF-I and R3 IGF-I mixture

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Acknowledgements

Thanks are addressed to Dr J Closset and Dr Ph Delahaut for providing IGF-I immuno-affinity columns. Thanks to Genentech for the gift of IGF-I. We also gratefully acknowledge Mr Pirens from Biocode-Hycel for standard comparison, Carlos Afonso for useful discussion, Pamela Brown and Jane Roberts HFL, UK for having assessed the protein-transposing software.

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Authors and Affiliations

  1. LCH, Laboratoire des Courses Hippiques, 15 rue de Paradis, 91370, Verrières le Buisson, France

    Marie-Agnes Popot & Patrice Garcia

  2. Centers for Disease Control & Prevention, 4770 Buford Highway, Atlanta, GA, 30341, USA

    Adrian R. Woolfitt

  3. Université Pierre. et Marie. Curie Paris 6, Structure et fonction de molécules bio actives, CNRS, UMR 7613, groupe de spectrométrie de masse, 4 place Jussieu, 75225, Paris Cedex 05, France

    Jean-Claude Tabet

Authors
  1. Marie-Agnes Popot
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  2. Adrian R. Woolfitt
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  3. Patrice Garcia
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  4. Jean-Claude Tabet
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Correspondence to Marie-Agnes Popot.

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Popot, MA., Woolfitt, A.R., Garcia, P. et al. Determination of IGF-I in horse plasma by LC electrospray ionisation mass spectrometry. Anal Bioanal Chem 390, 1843–1852 (2008). https://doi.org/10.1007/s00216-008-1889-z

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  • DOI: https://doi.org/10.1007/s00216-008-1889-z

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