Analytical and Bioanalytical Chemistry

, Volume 389, Issue 5, pp 1421–1428 | Cite as

Fourier transform ion cyclotron resonance mass spectrometry of covalent adducts of proteins and 4-hydroxy-2-nonenal, a reactive end-product of lipid peroxidation

  • Navin Rauniyar
  • Stanley M. StevensJr
  • Laszlo Prokai
Original Paper

Abstract

Covalent adduction of the model protein apomyoglobin by 4-hydroxy-2-nonenal, a reactive end-product of lipid peroxidation, was characterized by nanoelectrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (FTICR). The high mass resolving power and mass measurement accuracy of the instrument facilitated a detailed compositional analysis of the complex reaction product without the need for deconvolution and transformation to clearly show the pattern of adduction and component molecular weights. Our study has also demonstrated the value of electron capture dissociation over collision-induced dissociation for the tandem mass spectrometric determination of site modification for the 4-hydroxy-2-nonenal adduct of oxidized insulin B chain as an example.

Figure

FTICR allowed characterization of 4-hydroxy-2-nonenal (HNE)-modified apomyoglobin (an expanded spectrum of the +15 charge state is shown)

Keywords

Fourier transform ion cyclotron resonance mass spectrometry Electrospray ionization Protein carbonylation 4-Hydroxy-2-nonenal Electron capture dissociation 

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Navin Rauniyar
    • 1
  • Stanley M. StevensJr
    • 1
  • Laszlo Prokai
    • 1
  1. 1.Department of Molecular Biology & ImmunologyUniversity of North Texas Health, Science CenterFort WorthUSA

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