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Three-dimensional micro-XRF under cryogenic conditions: a pilot experiment for spatially resolved trace analysis in biological specimens

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Abstract

Three-dimensional micro-XRF is a recently developed microprobe which facilitates three-dimensional resolved chemical analyses with a resolution of around 20 μm. Arbitrary sites or sections of samples can be investigated without the need to section specimens physically. In this paper we demonstrate the use of the microprobe in combination with a cold nitrogen gas stream for the cryogenic fixation of specimens. A 3D micro-XRF setup at the new microfocus beamline at BESSY II was equipped with a nitrogen cryogenic stream. The distribution of Ca, Fe, Zn and Cu across virtual cross sections of a water-rich sample, the root of common duckweed, could be investigated without further sample preparation. This paper demonstrates the capabilities of 3D micro-XRF under cryogenic conditions for investigations of biological specimens.

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Acknowledgement

This work was partially funded by the Federal Ministry for Education and Research (BMBF) of Germany. We thank Udo Hesse for the photographs and Alexandra Graebert (HMI, Berlin) for her support in taking microscope images.

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Authors and Affiliations

  1. Institute for Optic and Atomic Physics, Technical University of Berlin, Hardenbergstr. 36, 10623, Berlin, Germany

    Birgit Kanngießer, Wolfgang Malzer, Marcel Pagels & Lars Lühl

  2. Institute for Applied Physical Chemistry, University of Heidelberg, Im Neuenheimer Feld 253, 69120, Heidelberg, Germany

    Gundolf Weseloh

Authors
  1. Birgit Kanngießer
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  2. Wolfgang Malzer
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  3. Marcel Pagels
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  4. Lars Lühl
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  5. Gundolf Weseloh
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Correspondence to Birgit Kanngießer.

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Kanngießer, B., Malzer, W., Pagels, M. et al. Three-dimensional micro-XRF under cryogenic conditions: a pilot experiment for spatially resolved trace analysis in biological specimens. Anal Bioanal Chem 389, 1171–1176 (2007). https://doi.org/10.1007/s00216-007-1494-6

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  • DOI: https://doi.org/10.1007/s00216-007-1494-6

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