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Trace metals in mussel shells and corresponding soft tissue samples: a validation experiment for the use of Perna perna shells in pollution monitoring

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Abstract

The uptake of Cr, Mn, Ni, Cu, Zn, Cd and Pb in soft tissue of Perna perna mussels and their shells has been studied in aquarium experiments in which mussels were exposed for 30 or 60 days to seawater spiked with different concentrations of these contaminants (125 and 500 μg L−1). Tissue samples were analyzed after acid digestion by conventional solution nebulization ICP–MS. Laser ablation ICP–MS was used for the quantitative determination of trace elements in different areas of the corresponding shells. With the exception of Mn and Zn, all other elements studied showed a significant concentration enhancements in soft tissue, with the magnitude of this enhancement following the order: Cr > Ni > Cd > Cu > Pb. A corresponding increase in most contaminants, although less pronounced, was also observed in the newly formed growth rings of mussel shells, contributing to the validation of Perna perna mussel shell as a bioindicator of toxic elements.

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Acknowledgements

This work was financially supported by FINEP (contract No. 65.92. 0012) and by Petrobrás. Both authors are grateful for research grants received from CAPES (V.R.B.) and CNPq (N.M.). We also gratefully acknowledge the dedication and skill of Álvaro J. Pereira in relation to the ICP–MS measurements, and the kind donation of a reference material by Dr. Noburo Imai.

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Authors and Affiliations

  1. CTTMAR (Center for Technology Earth and Ocean Science), Vale do Itajaí University (UNIVALI), Rua Uruguai 458, 88302-202, Itajaí, Brazil

    V. R. Bellotto

  2. Department of Chemistry, Pontifical Catholic University (PUC-Rio), Rua Marquês de São Vicente 225, 22451-900, Rio de Janeiro, Brazil

    N. Miekeley

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  1. V. R. Bellotto
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  2. N. Miekeley
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Correspondence to N. Miekeley.

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Bellotto, V.R., Miekeley, N. Trace metals in mussel shells and corresponding soft tissue samples: a validation experiment for the use of Perna perna shells in pollution monitoring. Anal Bioanal Chem 389, 769–776 (2007). https://doi.org/10.1007/s00216-007-1420-y

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  • DOI: https://doi.org/10.1007/s00216-007-1420-y

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