An integrated procedure for the measurement of total dietary fibre (including resistant starch), non-digestible oligosaccharides and available carbohydrates

Abstract

A method is described for the measurement of dietary fibre, including resistant starch (RS), non-digestible oligosaccharides (NDO) and available carbohydrates. Basically, the sample is incubated with pancreatic α-amylase and amyloglucosidase under conditions very similar to those described in AOAC Official Method 2002.02 (RS). Reaction is terminated and high molecular weight resistant polysaccharides are precipitated from solution with alcohol and recovered by filtration. Recovery of RS (for most RS sources) is in line with published data from ileostomy studies. The aqueous ethanol extract is concentrated, desalted and analysed for NDO by high-performance liquid chromatography by a method similar to that described by Okuma (AOAC Method 2001.03), except that for logistical reasons, d-sorbitol is used as the internal standard in place of glycerol. Available carbohydrates, defined as d-glucose, d-fructose, sucrose, the d-glucose component of lactose, maltodextrins and non-resistant starch, are measured as d-glucose plus d-fructose in the sample after hydrolysis of oligosaccharides with a mixture of sucrase/maltase plus β-galactosidase.

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References

  1. 1.

    DeVries JW (2004) J AOAC Int 87:682–706

    CAS  Google Scholar 

  2. 2.

    Asp N-G (2001) In: McCleary BV, Prosky L (eds) Advanced dietary fibre technology. Blackwell, Oxford

    Google Scholar 

  3. 3.

    Hipsley E (1953) Br Med J 2:420–422

    CAS  Article  Google Scholar 

  4. 4.

    Trowell HC, Southgate DAT, Wolever TMS, Leeds AR, Gassull MA, Jenkins DJA (1976) Lancet 1:967

    Article  CAS  Google Scholar 

  5. 5.

    Prosky L, Asp NG, Furda I, DeVries JW, Schweizer TF, Harland BF (1985) J AOAC Int 68:677–679

    CAS  Google Scholar 

  6. 6.

    Prosky L, Asp N-G, Schweizer TF, DeVries JW, Furda I, Lee SC (1994) J AOAC Int 77:690–694

    CAS  Google Scholar 

  7. 7.

    Lee SC, Prosky L, DeVries JW (1992) J AOAC Int 75:395–416

    CAS  Google Scholar 

  8. 8.

    Horwitz W (ed) (2002) Official methods of analysis of AOAC International, 17th edn. AOAC International, Gaithersberg

    Google Scholar 

  9. 9.

    Anonymous (2001) Cereal Foods World 46:112–126

    Google Scholar 

  10. 10.

    Englyst HN, Cummings JH (1984) Analyst 109:937–942

    Article  CAS  Google Scholar 

  11. 11.

    Englyst HN, Hudson GJ (1987) Food Chem 24:63–67

    Article  CAS  Google Scholar 

  12. 12.

    Englyst HN, Cummings JH (1988) J AOAC Int 71:808–814

    CAS  Google Scholar 

  13. 13.

    Englyst HN, Quigley ME, Hudson GJ (1994) Analyst 119:1497–1509

    Article  CAS  Google Scholar 

  14. 14.

    Southgate DAT (1969) J Sci Food Agric 20:326–330

    Article  CAS  Google Scholar 

  15. 15.

    Southgate DAT, Hudson GJ, Englyst HN (1978) J Sci Food Agric 29:979–988

    Article  CAS  Google Scholar 

  16. 16.

    Institute of Medicine (2002) In: Dietary reference intakes: energy, carbohydrates, fiber, fat, fatty acids, cholesterol, protein and amino acids. National Academies Press, Washington

  17. 17.

    Codex Committee on Nutrition and Foods for Special Dietary Uses (CCNFSD; ALINORM 06/29/26), 27th session, Bonn, Germany, 21–25 November 2005

  18. 18.

    WHO/FAO meeting of carbohydrates experts, Geneva, 17–18 July, 2006. Report CRD 19

  19. 19.

    Codex Committee on Nutrition and Foods for Special Dietary Uses, 28th session, Chiang Mai, Thailand, 30 October–3 November 2006

  20. 20.

    Hoebregs H (1997) J AOAC Int 80:1029–1037

    CAS  Google Scholar 

  21. 21.

    McCleary BV, McNally M, Rossiter P (2002) J AOAC Int 85:1103–1111

    CAS  Google Scholar 

  22. 22.

    Craig SAS, Holden JF, Khaled MJ (2000) J AOAC Int 83:1006–1012

    CAS  Google Scholar 

  23. 23.

    Gordon DT, Okuma K (2002) J AOAC Int 85:435–444

    CAS  Google Scholar 

  24. 24.

    De Slegte J (2002) J AOAC Int 85:417–423

    Google Scholar 

  25. 25.

    McCleary BV, McNally M, Rossiter P (2002) J AOAC Int 85:1103–1111

    CAS  Google Scholar 

  26. 26.

    Dietary Fibre, 2006 Helsinki, 11–14 June 2006

  27. 27.

    McCleary BV, Monaghan DA (2002) J AOAC Int 85:665–675

    CAS  Google Scholar 

  28. 28.

    McCleary BV, Rossiter P (2004) J AOAC Int 87:707–717

    CAS  Google Scholar 

  29. 29.

    Gibson GR, Beatry ER, Wang X, Cummings JH (1995) Gastroenterology 108:975–982

    Article  CAS  Google Scholar 

  30. 30.

    Zhong J, Luo B, Xiang M, Liu H, Zhai Z, Wang T, Craig SAS (2000) Am J Clin Nutr 72:1503–1509

    CAS  Google Scholar 

  31. 31.

    Tomlin J, Read NW (1988) Aliment Pharmacol Ther 2:513–519

    CAS  Article  Google Scholar 

  32. 32.

    Endo K, Kumemura M, Nakamura K, Fujisawa T, Suzuki K, Benno Y, Mitsuoka T (1991) Bifidobact Microflora 10:53–64

    Google Scholar 

  33. 33.

    Satouchi M, Wakabayashi S, Ohkuma K, Fujiwara K, Matsuoka A (1993) Jpn J Nutr 51(1):31–37

    CAS  Google Scholar 

  34. 34.

    Deguchi Y, Matsumoto K, Ito A, Watanuki M (1997) Jpn J Nutr 55(1):13–22

    CAS  Google Scholar 

  35. 35.

    Teuri U, Korpela R (1998) Ann Nutr Metab 42:319–327

    Article  CAS  Google Scholar 

  36. 36.

    Takahashi H, Yang SI, Hayashi C, Kim M, Yamanaka J, Yamamoto T (1993) Nutr Res 13:649–657

    Article  CAS  Google Scholar 

  37. 37.

    Kleessen B, Sykura B, Zunft H-J, Blaut M (1997) Am J Clin Nutr 65:1397–1402

    CAS  Google Scholar 

  38. 38.

    Davidson MH, Dugan LD, Burns JH, Bova J, Story K, Drennan KB (1991) JAMA 265(14):1833–1839

    Article  CAS  Google Scholar 

  39. 39.

    Anderson JW, Gustafson NJ, Bryant CA, Tietyn-Clark J (1987) J Am Diet Assoc 87(9):1189–1197

    CAS  Google Scholar 

  40. 40.

    Rivellese A, Riccardi G, Giacco A, Pacioni D, Genovese S, Mattioli PL, Mancini M (1980) Lancet 2:447–450

    Article  CAS  Google Scholar 

  41. 41.

    Wolever TMS (1990) In: Kritchevsky D, Bonfield C, Anderson JW (eds) Dietary fiber: chemistry, physiology, and health effects. Plenum, New York

    Google Scholar 

  42. 42.

    Jenkins DJA, Jenkins AL, Wolever TMS, Vuksan V (1990) In: Furda I, Brine CJ (eds) New developments in dietary fiber: physiological, physicochemical and analytical aspects. Plenum, New York

    Google Scholar 

  43. 43.

    Jenkins DJA, Leeds AR, Gassull MA, Cochet B, Alberti KGM (1977) Ann Int Med 86:20–23

    CAS  Google Scholar 

  44. 44.

    Anderson JW, Gustafson NJ, Bryant CA, Tietyn-Clark J (1987) J Am Diet Assoc 87(9):1189–1197

    CAS  Google Scholar 

  45. 45.

    Jenkins DJA, Wolever TMS, Talor RH, Barker H, Fielden H, Baldwin JM, Bowling AC, Newman HC, Jenkins AL, Goff DV (1981) Am J Clin Nutr 34:362–366

    CAS  Google Scholar 

  46. 46.

    Demark-Wahnefried W, Bowering J, Cohen PS (1990) J Am Diet Assoc 90:2223–2229

    Google Scholar 

  47. 47.

    Kirby RW, Anderson JW, Sieling B, Rees ED, Chen W-JL, Miller RE, Kay RM (1981) Am J Clin Nutr 34:824–829

    CAS  Google Scholar 

  48. 48.

    Van Horn L, Emidy LA, Liu K, Liao Y, Ballew C, King J, Stamler J (1988) Prev Med 17:377–386

    Article  Google Scholar 

  49. 49.

    Munoz JM, Sandstead HH, Jacob RA, Logan GM Jr, Reck SJ, Klevay LM, Dintzis FR, Inglett GE, Shuey WC (1979) Am J Clin Nutr 32:580–592

    CAS  Google Scholar 

  50. 50.

    Hunninghake DB, Miller VT, LaRosa JC, Kinosian B, Brown V, Howard WJ, DiSerio FJ, O’Connor RR (1994) Am J Clin Nutr 59:1050–1054

    CAS  Google Scholar 

  51. 51.

    Lia A, Hallman G, Sandberg AS, Sundberg B, Aman P, Anderson H (1995) J Clin Nutr 62:1245–1251

    CAS  Google Scholar 

  52. 52.

    Yamashita K, Kawai K, Itakura M (1984) Nutr Res 4:961–966

    Article  CAS  Google Scholar 

  53. 53.

    McCleary BV, Rossiter PC (2006) Egan Press Publications (AACC International) (in press)

  54. 54.

    Berry CS (1986) J Cereal Sci 4:301–314

    CAS  Article  Google Scholar 

  55. 55.

    Englyst HN, Kingman SM, Cummings JH (1992) Eur J Clin Nutr 46(Suppl.2):S33–S50

    Google Scholar 

  56. 56.

    Champ M (1992) Eur J Clin Nutr 46(Suppl. 2):S51–S62

    Google Scholar 

  57. 57.

    Goni I, Garcia-Diz E, Manas E, Saura-Calixto F (1996) Food Chem 56:445–449

    Article  CAS  Google Scholar 

  58. 58.

    Faisant N, Planchot V, Kozlowski F, Pacouret M-P, Colonna P, Champ M (1995) Sci Aliments 15:83–89

    CAS  Google Scholar 

  59. 59.

    Leeds A, Brand Miller J, Foster-Powell K, Colagiuri S (2003) The new glucose revolution. Hodder and Stoughton, London

    Google Scholar 

  60. 60.

    Lee SC, Prosky L (1995) J AOAC Int 78:22–36

    CAS  Google Scholar 

  61. 61.

    Cho SS, Prosky L (1999) In: Cho SS, Prosky L, Dreher M (eds) Complex carbohydrates in foods. Dekker, New York

    Google Scholar 

  62. 62.

    Brighenti F, Casiraghi MC, Canzi E, Ferrari A (1999) Eur J Clin Nutr 553(9):726–733

    Article  CAS  Google Scholar 

  63. 63.

    Causey JL, Feirtag JM, Gallaher DD, Tungland BC, Slavin JL (2000) Nutr Res 20(2):191–201

    Article  CAS  Google Scholar 

  64. 64.

    Takahashi H, Wako N, Okubo T, Ishihara N, Yamanaka J, Yamamoto T (1994) J Nutr Sci Vitaminol 40:251–259

    CAS  Google Scholar 

  65. 65.

    Nomura M, Nakajima Y, Abe H (1992) J Jpn Soc Nutr Food Sci 45:21–25

    Google Scholar 

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Acknowledgements

The author thanks Patricia Rossiter and Anna Dragar for performing the RS analyses and HPLC of LMWNDO. TDF determinations were performed by Luzmila Ludena, Bread Research Institute Ltd., North Ryde, NSW, Australia.

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Correspondence to Barry V. McCleary.

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McCleary, B.V. An integrated procedure for the measurement of total dietary fibre (including resistant starch), non-digestible oligosaccharides and available carbohydrates. Anal Bioanal Chem 389, 291–308 (2007). https://doi.org/10.1007/s00216-007-1389-6

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Keywords

  • Total dietary fibre
  • Resistant starch
  • Non-digestible oligosaccharides
  • Available carbohydrates
  • Integrated method