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Determination of molar absorptivity coefficients for major type-B trichothecenes and certification of calibrators for deoxynivalenol and nivalenol

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Abstract

This paper presents results from the European Commission-funded project Doncalibrant, the objective of which was to produce calibrators with certified mass fractions of the Fusarium toxins deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-Ac-DON), 15-acetyldeoxynivalenol (15-Ac-DON), and nivalenol (NIV), in acetonitrile. The calibrators, available in ampoules, were sufficiently homogeneous, with between-bottle variations (s bb) of less than 2%. Long-term stability studies performed at four different temperatures between −18 and 40 °C revealed no significant negative trends (at a confidence level of 95%). Molar absorptivity coefficients (in L mol−1 cm−1) were determined for all four toxins (DON: 6805 ± 126, NIV: 6955 ± 205, 3-Ac-DON: 6983 ± 141, 15-Ac-DON: 6935 ± 142) on the basis of a mini-interlaboratory exercise. The overall uncertainty of the calibrators’ target values for DON and NIV were evaluated on the basis of gravimetric preparation data and include uncertainty contributions from possible heterogeneity, storage, and transport. The Doncalibrant project resulted in the production of calibrators for DON (IRMM-315) and NIV (IRMM-316) in acetonitrile with certified mass fractions of 25.1 ± 1.2 μg g−1 and 24.0 ± 1.1 μg g−1, respectively. Both CRMs became commercially available from the Institute for Reference Materials and Measurements (IRMM, Geel, Belgium) at the beginning of 2007.

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Acknowledgments

The authors want to thank the processing team at IRMM for its contribution to the production of the reference materials, and the European Commission for financial support under Project No. GRD1-2002-70018.

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Author notes

  1. Ralf D. Josephs

    Present address: Bureau International des Poids et Mesures (BIPM), Pavillon de Breteuil, 92312, Sèvres, France

Authors and Affiliations

  1. Centre for Analytical Chemistry, University of Natural Resources and Applied Life Sciences Vienna, Department for Agrobiotechnology (IFA Tulln), Konrad Lorenz Straße 20, 3430, Tulln, Austria

    Rudolf Krska & Patricia Schubert-Ullrich

  2. European Commission, DG Joint Research Centre (JRC), Institute for Reference Materials and Measurements (IRMM), Retieseweg 111, 2440, Geel, Belgium

    Ralf D. Josephs, Håkan Emteborg & Gerhard Buttinger

  3. Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences (SLU), P.O. Box 7024, 75007, Uppsala, Sweden

    Hans Pettersson

  4. National Institute of Public Health and the Environment (RIVM), Laboratory of Residue Analysis (ARO), P.O. Box 1, 3720, Bilthoven, The Netherlands

    Hans P. van Egmond & Ronald C. Schothorst

  5. Department of Environment, Food and Rural Affairs, Central Science Laboratory (CSL), Sand Hutton, York, YO41 1LZ, UK

    Susan MacDonald & Danny Chan

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  1. Rudolf Krska
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  2. Patricia Schubert-Ullrich
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  4. Håkan Emteborg
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  6. Hans Pettersson
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  7. Hans P. van Egmond
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  8. Ronald C. Schothorst
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  9. Susan MacDonald
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  10. Danny Chan
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Correspondence to Rudolf Krska.

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Krska, R., Schubert-Ullrich, P., Josephs, R.D. et al. Determination of molar absorptivity coefficients for major type-B trichothecenes and certification of calibrators for deoxynivalenol and nivalenol. Anal Bioanal Chem 388, 1215–1226 (2007). https://doi.org/10.1007/s00216-007-1369-x

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  • DOI: https://doi.org/10.1007/s00216-007-1369-x

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