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Modelling of the impedimetric responses of an aflatoxin B1 immunosensor prepared on an electrosynthetic polyaniline platform

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Abstract

Aflatoxins are a group of mycotoxins that have deleterious effects on humans and are produced during fungal infection of plants or plant products. An electrochemical immunosensor for the determination of aflatoxin B1 (AFB1) was developed with AFB1antibody (AFB1-Ab) immobilized on Pt electrodes modified with polyaniline (PANi) and polystyrene sulphonic acid (PSSA). Impedimetric analysis shows that the electron transfer resistances of the Pt/PANi–PSSA electrode, the Pt/PANi–PSSA/AFB1-Ab immunosensor and Pt/PANi–PSSA/AFB1-Ab incubated in bovine serum albumin (BSA) were 0.458, 720 and 1,066 kΩ, respectively. These results indicate that electrochemical impedance spectroscopy (EIS) is a suitable method for monitoring the change in electron transfer resistance associated with the immobilization of the antibody. Modelling of EIS data gave equivalent circuits which showed that the electron transfer resistance increased from 0.458 kΩ for the Pt/PANi–PSSA electrode to 1,066 kΩ for the Pt/PANi–PSSA/AFB1-Ab immunosensor, indicating that immobilization of the antibody and incubation in BSA introduced an electron transfer barrier. The AFB1 immunosensor had a detection limit of 0.1 mg/L and a sensitivity of 869.6 kΩ L/mg.

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Authors and Affiliations

  1. Sensor Research Laboratory, Department of Chemistry, University of the Western Cape, Private Bag X17, Bellville, 7535, Cape Town, South Africa

    Joseph H. O. Owino, Anna Ignaszak, Amir Al-Ahmed, Priscilla G. L. Baker & Emmanuel I. Iwuoha

  2. Chemistry Department, National University of Lesotho, P.O. Roma 180, Lesotho, South Africa

    Hailemichael Alemu

  3. School of Chemistry, University of KwaZulu-Natal, Westville Campus, Private Bag X54001, Westville, Durban, 4000, South Africa

    Jane Catherine Ngila

Authors
  1. Joseph H. O. Owino
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  2. Anna Ignaszak
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  3. Amir Al-Ahmed
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  4. Priscilla G. L. Baker
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  5. Hailemichael Alemu
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  6. Jane Catherine Ngila
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  7. Emmanuel I. Iwuoha
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Corresponding author

Correspondence to Emmanuel I. Iwuoha.

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Owino, J.H.O., Ignaszak, A., Al-Ahmed, A. et al. Modelling of the impedimetric responses of an aflatoxin B1 immunosensor prepared on an electrosynthetic polyaniline platform. Anal Bioanal Chem 388, 1069–1074 (2007). https://doi.org/10.1007/s00216-007-1333-9

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  • DOI: https://doi.org/10.1007/s00216-007-1333-9

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