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Simultaneous determination of p-hydroxyacetophenone, chlorogenic acid, and caffeic acid in Herba Artemisiae Scopariae by capillary electrophoresis with electrochemical detection

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Abstract

Capillary electrophoresis with electrochemical detection has been employed for the determination of p-hydroxyacetophenone, chlorogenic acid, and caffeic acid in Herba Artemisiae Scopariae (the dried sprout of Artemisia scoparia Waldst. et Kit.). The effects of several important factors, such as the concentration and the acidity of the running buffer, separation voltage, injection time, and detection potential, were investigated to acquire the optimum conditions. The detection electrode was a 300-μm-diameter carbon disc electrode at a working potential of +0.90 V (relative to the saturated calomel electrode). The three analytes can be well separated within 11 min in a 40-cm-long fused-silica capillary at a separation voltage of 15 kV in 50 mM borate buffer (pH 9.2). The relation between peak current and analyte concentration was linear over about 3 orders of magnitude, with detection limits (signal-to-noise ratio of 3) of 0.31, 0.39, and 0.50 μM for p-hydroxyacetophenone, chlorogenic acid, and caffeic acid, respectively. The proposed method has been successfully applied to monitor the three bioactive constituents in real plant samples and to differentiate between different herbal drugs with satisfactory assay results.

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Acknowledgements

This work was supported financially by NSFC (20675017 and 20405002), the Shanghai Science Committee (051107089 and 2004ZR140150212), the State Education Ministry of China, and the 863 Program of China (2004AA639740).

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Authors and Affiliations

  1. School of Pharmacy, Fudan University, Shanghai, 200032, China

    Xiao Yao & Gang Chen

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  1. Xiao Yao
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  2. Gang Chen
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Correspondence to Gang Chen.

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Yao, X., Chen, G. Simultaneous determination of p-hydroxyacetophenone, chlorogenic acid, and caffeic acid in Herba Artemisiae Scopariae by capillary electrophoresis with electrochemical detection. Anal Bioanal Chem 388, 475–481 (2007). https://doi.org/10.1007/s00216-007-1198-y

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  • DOI: https://doi.org/10.1007/s00216-007-1198-y

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