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Immobilization of hemoglobin on SBA-15 applied to the electrocatalytic reduction of H2O2

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Abstract

The direct electron transfer between hemoglobin (Hb) and an electrode was realized by first immobilizing the protein onto SBA-15.The results of the immobilization showed that the adsorption was pH-dependent with a maximum adsorption near the isoelectric point of the protein, and SBA-15 with a larger pore diameter showed greater adsorption capacity for Hb. UV–vis spectroscopy and nitrogen adsorption analysis indicated that Hb was adsorbed within the channel of SBA-15 and no significant denaturation occurred to the protein. The Hb/SBA-15 composite obtained was used for the fabrication of a Hb biosensor to detect hydrogen peroxide. A pair of well-defined redox peaks at −0.337 and −0.370 V on the Hb/SBA-15 composite modified glassy carbon electrode was observed, and the electrode reactions showed a surface-controlled process with a single proton transfer at a scan rate range from 20 to 1,000 mV/s. The sensor showed a fast amperometric response, a low detection limit (2.3 × 10−9 M) and good stability for the detection of H2O2. The electrochemical results indicated that the immobilized Hb still retained its biological activity.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (grant nos. 20635020, 20575026 and 90606016), the National Basic Research Program of China (grant no. 2003CB615804), Jiangsu National Natural Science Foundation (BK2006114) and the Modern Analysis Center of Nanjing University.

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Authors and Affiliations

  1. Key Laboratory of Analytical Chemistry for Life Science, Key Laboratory of Mesoscopic Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, People’s Republic of China

    Yuge Liu, Qin Xu, Xiaomiao Feng, Jun-Jie Zhu & Wenhua Hou

Authors
  1. Yuge Liu
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  2. Qin Xu
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  3. Xiaomiao Feng
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  4. Jun-Jie Zhu
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  5. Wenhua Hou
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Correspondence to Jun-Jie Zhu or Wenhua Hou.

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Liu, Y., Xu, Q., Feng, X. et al. Immobilization of hemoglobin on SBA-15 applied to the electrocatalytic reduction of H2O2 . Anal Bioanal Chem 387, 1553–1559 (2007). https://doi.org/10.1007/s00216-006-1064-3

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  • DOI: https://doi.org/10.1007/s00216-006-1064-3

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