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Quantification of Lactobacillus in fermented milk by multivariate image analysis with least-squares support-vector machines

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Abstract

This paper reports an approach for quantification of Lactobacillus in fermented milk, grown in a selective medium (MRS agar), by use of digital colour images of Petri plates easily obtained by use of a flatbed scanner. A one-dimensional data vector was formed to characterize each digital image on the basis of the frequency-distribution curves of the red (R), green (G), and blue (B) colour values, and quantities derived from them, for example lightness (L), relative red (RR), relative green (RG), and relative blue (RB). The frequency distributions of hue, saturation, and intensity (HSI) were also calculated and included in the data vector used to describe each image. Multivariate non-linear modelling using the least-squares support vector machine (LS-SVM) and a linear model based on PLS regression were developed to relate the microbiological count and the frequency vector. Feasibly models were developed using the LS-SVM and errors were below than 10% for Lactobacillus quantification, indicating the proposed approach can be used for automatic counting of colonies.

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Acknowledgements

The authors wish to thank the CNPq, FAPEMIG and CAPES for financial support.

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Authors and Affiliations

  1. Chemistry Institute, Campinas State University, P.O. Box 6154, 13084-971, Campinas, SP, Brazil

    Alessandra Borin, Lívia Cordi, Luiz C. M. Pataca, Nelson Durán & Ronei J. Poppi

  2. Chemistry and Physics Department, Santa Cruz do Sul University, C.P. 188, 96815-900, Santa Cruz do Sul, RS, Brazil

    Marco Flôres Ferrão

  3. Chemistry Institute, Franca University, C.P. 32, 14404-600, Franca, SP, Brazil

    Cesar Mello

  4. Environmental Science Center, Mogi das Cruzes University, 08780-911, Mogi das Cruzes, SP, Brazil

    Nelson Durán

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  1. Alessandra Borin
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  2. Marco Flôres Ferrão
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  3. Cesar Mello
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  4. Lívia Cordi
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  5. Luiz C. M. Pataca
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  6. Nelson Durán
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  7. Ronei J. Poppi
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Correspondence to Ronei J. Poppi.

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Borin, A., Ferrão, M.F., Mello, C. et al. Quantification of Lactobacillus in fermented milk by multivariate image analysis with least-squares support-vector machines. Anal Bioanal Chem 387, 1105–1112 (2007). https://doi.org/10.1007/s00216-006-0971-7

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  • DOI: https://doi.org/10.1007/s00216-006-0971-7

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