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Application of an in-line imprinted polymer column in a potentiometric flow-injection chemical sensor to the determination of the carbamate pesticide carbaryl in complex biological matrices

Analytical and Bioanalytical Chemistry volume 387, pages 351–357 (2007)Cite this article

Abstract

A flow-injection biosensor-like system based on a nonenzymatic approach has been developed to determine the carbamate pesticide carbaryl in complex biological samples without lengthy and expensive extraction steps. Molecularly imprinted polymeric beads were used to immobilize carbaryl from biological samples. pH variation permitted the elution of carbaryl from the binding cavity to the flow cell. A pH electrode was used to detect changes in the charge of carbaryl in the sample solution resulting from the protonation and deprotonation of the molecule over different pH ranges. At pH 2.0, the secondary amine group is protonated, giving a (+1) charge to the carbaryl molecule. At pH 8.0, the ionized carbaryl loses a proton to become neutral, changing the local pH of the flow cell. The pH change at the flow cell generated by the deprotonation of carbaryl ion in alkaline medium was used to determine the carbaryl concentration. Parameters influencing the performance of the system were optimized for use in the detection procedure. The validated biosensor-like system had a carbaryl detection limit of 10.0 μg/mL and a response that was linear (r 2 > 0.98) over the concentration range of 10.0–00 μg/mL.

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Acknowledgement

This work was made possible by the support of Charles River Laboratories Preclinical Services Montreal, Inc. (CRM) and Concordia University.

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Affiliations

  1. Charles River Laboratories Preclinical Services Montreal, Inc., 87 Senneville Road, Senneville, Québec, H9X 3R3, Canada

    Jamil Hantash, Alan Bartlett, Philip Oldfield, Roger O’Rielly & Francois David

  2. Laboratory of Solid State Chemistry and Mössbauer Spectroscopy, Department of Chemistry and Biochemistry, Concordia University, 7141 Sherbrooke Street West, Montréal, Québec, H4B 1R6, Canada

    Jamil Hantash & Georges Dénès

Authors
  1. Jamil Hantash
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  2. Alan Bartlett
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  3. Philip Oldfield
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  4. Georges Dénès
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  5. Roger O’Rielly
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  6. Francois David
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Correspondence to Jamil Hantash.

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Hantash, J., Bartlett, A., Oldfield, P. et al. Application of an in-line imprinted polymer column in a potentiometric flow-injection chemical sensor to the determination of the carbamate pesticide carbaryl in complex biological matrices. Anal Bioanal Chem 387, 351–357 (2007). https://doi.org/10.1007/s00216-006-0898-z

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  • DOI: https://doi.org/10.1007/s00216-006-0898-z

Keywords

  • Pesticides
  • Potentiometric
  • Flow injection
  • Biosensor-like
  • Molecularly imprinted polymer
  • Suspension polymerization
  • Detection