Skip to main content
Log in

Single-molecule tracking in eukaryotic cell nuclei

  • Trends
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2


  1. Sako Y, Yanagida T (2003) Nat Cell Biol Ss1–Ss5

  2. Tinnefeld P, Sauer M (2005) Angew Chem Int Ed Engl 44:2642–2671

    Article  CAS  Google Scholar 

  3. Kues T, Peters R, Kubitscheck U (2001) Biophys J 80:2954–2967

    CAS  Google Scholar 

  4. Michalet X, Pinaud FF, Bentolila LA, Tsay JM, Doose S, Li JJ, Sundaresan G, Wu AM, Gambhir SS, Weiss S (2005) Science 307:538–544

    Article  CAS  Google Scholar 

  5. Harms GS, Cognet L, Lommerse PH, Blab GA, Schmidt T (2001) Biophys J 80:2396–2408

    Article  CAS  Google Scholar 

  6. Steinmeyer R, Noskov A, Krasel C, Weber I, Dees C, Harms GS (2005) J Fluoresc 15:707–721

    Article  CAS  Google Scholar 

  7. Thompson RE, Larson DR, Webb WW (2002) Biophys J 82:2775–2783

    CAS  Google Scholar 

  8. Sage D, Neumann FR, Hediger F, Gasser SM, Unser M (2005) IEEE Trans Image Process 14:1372–1383

    Article  Google Scholar 

  9. Bausinger R, von Gersdorff K, Braeckmans K, Ogris M, Wagner E, Brauchle C, Zumbusch A (2006) Angew Chem Int Ed Engl 45:1568–1572

    Article  CAS  Google Scholar 

  10. Saxton MJ, Jacobson K (1997) Ann Rev Biophys Biomol Struct 26:373–399

    Article  CAS  Google Scholar 

  11. Shav-Tal Y, Darzacq X, Shenoy SM, Fusco D, Janicki SM, Spector DL, Singer RH (2004) Science 304:1797–1800

    Article  CAS  Google Scholar 

  12. Kubitscheck U, Grunwald D, Hoekstra A, Rohleder D, Kues T, Siebrasse JP, Peters R (2005) J Cell Biol 168:233–243

    Article  CAS  Google Scholar 

  13. Goulian M, Simon SM (2000) Biophys J 79:2188–2198

    CAS  Google Scholar 

  14. Grunwald D, Spottke B, Buschmann V, Kubitscheck U (2006) Mol Biol Cell (in press)

Download references


U.K. gratefully acknowledges financial support by the Volkswagen Foundation (Grant I/78852) and the German Research Foundation (Grant Ku 1507/1-2).

Author information

Authors and Affiliations


Corresponding author

Correspondence to Ulrich Kubitscheck.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Single U1 snRNPs diffusing and binding within a living HeLa cell nucleus. Left hand side original but contrast-enhanced data. Right hand side movie data was filtered using a spot-enhancing filter (3-pixel diameter, see ref. [8]) to improve U1 snRNP visibility. The movie was recorded at 200 Hz, the total object field corresponded to an area of 12.2×12.2 μm2 (AVI 3.78 mb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Siebrasse, J.P., Grünwald, D. & Kubitscheck, U. Single-molecule tracking in eukaryotic cell nuclei. Anal Bioanal Chem 387, 41–44 (2007).

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: