Skip to main content
Log in

Single-molecule tracking in eukaryotic cell nuclei

  • Trends
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

References

  1. Sako Y, Yanagida T (2003) Nat Cell Biol Ss1–Ss5

  2. Tinnefeld P, Sauer M (2005) Angew Chem Int Ed Engl 44:2642–2671

    Article  CAS  Google Scholar 

  3. Kues T, Peters R, Kubitscheck U (2001) Biophys J 80:2954–2967

    CAS  Google Scholar 

  4. Michalet X, Pinaud FF, Bentolila LA, Tsay JM, Doose S, Li JJ, Sundaresan G, Wu AM, Gambhir SS, Weiss S (2005) Science 307:538–544

    Article  CAS  Google Scholar 

  5. Harms GS, Cognet L, Lommerse PH, Blab GA, Schmidt T (2001) Biophys J 80:2396–2408

    Article  CAS  Google Scholar 

  6. Steinmeyer R, Noskov A, Krasel C, Weber I, Dees C, Harms GS (2005) J Fluoresc 15:707–721

    Article  CAS  Google Scholar 

  7. Thompson RE, Larson DR, Webb WW (2002) Biophys J 82:2775–2783

    CAS  Google Scholar 

  8. Sage D, Neumann FR, Hediger F, Gasser SM, Unser M (2005) IEEE Trans Image Process 14:1372–1383

    Article  Google Scholar 

  9. Bausinger R, von Gersdorff K, Braeckmans K, Ogris M, Wagner E, Brauchle C, Zumbusch A (2006) Angew Chem Int Ed Engl 45:1568–1572

    Article  CAS  Google Scholar 

  10. Saxton MJ, Jacobson K (1997) Ann Rev Biophys Biomol Struct 26:373–399

    Article  CAS  Google Scholar 

  11. Shav-Tal Y, Darzacq X, Shenoy SM, Fusco D, Janicki SM, Spector DL, Singer RH (2004) Science 304:1797–1800

    Article  CAS  Google Scholar 

  12. Kubitscheck U, Grunwald D, Hoekstra A, Rohleder D, Kues T, Siebrasse JP, Peters R (2005) J Cell Biol 168:233–243

    Article  CAS  Google Scholar 

  13. Goulian M, Simon SM (2000) Biophys J 79:2188–2198

    CAS  Google Scholar 

  14. Grunwald D, Spottke B, Buschmann V, Kubitscheck U (2006) Mol Biol Cell (in press)

Download references

Acknowledgements

U.K. gratefully acknowledges financial support by the Volkswagen Foundation (Grant I/78852) and the German Research Foundation (Grant Ku 1507/1-2).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Ulrich Kubitscheck.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Single U1 snRNPs diffusing and binding within a living HeLa cell nucleus. Left hand side original but contrast-enhanced data. Right hand side movie data was filtered using a spot-enhancing filter (3-pixel diameter, see ref. [8]) to improve U1 snRNP visibility. The movie was recorded at 200 Hz, the total object field corresponded to an area of 12.2×12.2 μm2 (AVI 3.78 mb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Siebrasse, J.P., Grünwald, D. & Kubitscheck, U. Single-molecule tracking in eukaryotic cell nuclei. Anal Bioanal Chem 387, 41–44 (2007). https://doi.org/10.1007/s00216-006-0763-0

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-006-0763-0

Keywords

Navigation