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Fluorescence determination of metoprolol in human plasma by trilinear decomposition-based calibration techniques

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In this study a new spectrofluorimetric method for the direct determination of metoprolol in human plasma is presented and discussed. It is based on the use of fluorescence excitation–emission matrices (EEMs) and second-order calibration performed with parallel factor analysis (PARAFAC) or alternating trilinear decomposition (ATLD). This methodology enables accurate and reliable discrimination of the analyte signal, even in the presence of unknown and uncalibrated fluorescent component(s), which is often referred to as the second-order advantage. No separation or sample pretreatment steps were required. Satisfactory results were obtained. Metoprolol recoveries in plasma were determined as 87±2% and 90±4% with PARAFAC and ATLD, respectively. All RSD values of intra- and interday assays were below 5%.

A three-dimensional plot of EEMs for a plasma sample and metoprolol solution

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  1. Bristow MR (2000) Circulation 101:558–569

    CAS  Google Scholar 

  2. Reiter MJ, Reiffel JA (1998) Am J Cardiol 82:9I–19I

    Article  CAS  Google Scholar 

  3. Modamio P, Lastra CF, Mariño EL (1998) J Pharm Biomed Anal 17:507–513

    Article  CAS  Google Scholar 

  4. Basci NE, Temizer A, Bozkurt A, Isimer A (1998) J Pharm Biomed Anal 18:745–750

    Article  CAS  Google Scholar 

  5. Braza AJ, Modamio P, Lastra CF, Mariño EL (2002) Biomed Chromatogr 16:517–522

    Article  CAS  Google Scholar 

  6. Albers S, Elshoff JP, Völker C, Richter A, Läer S (2005) Biomed Chromatogr 19:202–207

    Article  CAS  Google Scholar 

  7. Gergov M, Robson JN, Duchoslav E, Ojanperä I (2000) J Mass Spectrom 35:912–918

    Article  CAS  Google Scholar 

  8. Thevis M, Opfermann G, Schänzer W (2001) Biomed Chromatogr 15:393–402

    Article  CAS  Google Scholar 

  9. Kataoka H, Narimatsu S, Lord HL, Pawliszyn J (1999) Anal Chem 71:4237–4244

    Article  CAS  Google Scholar 

  10. Naidong W, Shou WZ, Addison T, Maleki S, Jiang XY (2002) Rapid Commun Mass Spectrom 16:1965–1975

    Article  CAS  Google Scholar 

  11. Bro R (1997) Chemom Intell Lab Syst 38:149–171

    Article  CAS  Google Scholar 

  12. Wu HL, Shibukawa M, Oguma K (1998) J Chemom 12:1–26

    Article  CAS  Google Scholar 

  13. Booksh KS, Kowalski BR (1994) Anal Chem 66:782A–791A

    Article  CAS  Google Scholar 

  14. Damiani PC, Nepote AJ, Bearzotti M, Olivieri AC (2004) Anal Chem 76:2798–2806

    Article  CAS  Google Scholar 

  15. Muñoz de la Peña A, Espinosa Mansilla A, González Gómez D, Olivieri AC, Goicoechea HC (2003) Anal Chem 75:2640–2646

    Article  Google Scholar 

  16. Espinosa Mansilla A, Muñoz de la Peña A, González Gómez D, Salinas F (2005) Anal Chim Acta 531:257–266

    Article  CAS  Google Scholar 

  17. Esteves da Silva JCG, Leitão JMM, Costa FS, Ribeiro JLA (2002) Anal Chim Acta 453:105–115

    Article  CAS  Google Scholar 

  18. Arancibia JA, Escandar GM (2003) Talanta 60:1113–1121

    Article  CAS  Google Scholar 

  19. Messick NJ, Kalivas JH, Lang PM (1996) Anal Chem 68:1572–1579

    Article  CAS  Google Scholar 

  20. Boqué R, Ferré J, Faber NM, Rius FX (2002) Anal Chim Acta 451:313–321

    Article  Google Scholar 

  21. Bro R, Kiers HAL (2003) J Chemom 17:274–286

    Article  CAS  Google Scholar 

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This work was financially supported by the National Natural Science Foundation of China (grant No. 20475014, 20375012).

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Correspondence to Hai-Long Wu.

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Zhang, Y., Wu, HL., Xia, AL. et al. Fluorescence determination of metoprolol in human plasma by trilinear decomposition-based calibration techniques. Anal Bioanal Chem 386, 1741–1748 (2006).

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