Abstract
A reference material for the biological monitoring of occupational exposure to toluene, benzene and phenol was prepared. O-cresol and hippuric acid (metabolites of toluene) are used for the biological monitoring of occupational exposure to toluene. Phenol, a metabolite of benzene, is used for the biological monitoring of exposure to benzene, but phenol can of course also be used as an indicator of exposure to phenol as well. The reference material (RM) used for the determination of these metabolites was prepared by freeze-drying pooled urine samples obtained from healthy persons occupationally exposed to toluene and those taking part in an inhalation experiment. Tests for homogeneity and stability were performed by determining urine concentrations of o-cresol, hippuric acid, creatinine and phenol. To investigate the stability of the RM, the urinary concentrations of o-cresol and phenol were monitored for eighteen months using GC and HPLC, while those of hippuric acid and creatinine were followed for five and six years, respectively, using HPLC. Analysis of variance showed that the concentrations did not change. The certified concentration values (and their uncertainties) of the substances in this reference material (phenol concentration c=6.46±0.58 mg l−1; o-cresol concentration c=1.17±0.15 mg l−1; hippuric acid concentration c=1328±30 mg l−1; creatinine concentration c=0.82±0.10 g l−1) were evaluated via the interactive statistical programme IPECA.
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This work was supported by the Internal Grant Agency of Ministry of Health of the Czech Republic (Grant NR/7831-3).
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Šperlingová, I., Dabrowská, L., Stránský, V. et al. Human urine certified reference material CZ 6010: creatinine and toluene metabolites (hippuric acid and o-cresol) and a benzene metabolite (phenol). Anal Bioanal Chem 387, 2419–2424 (2007). https://doi.org/10.1007/s00216-006-0708-7
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DOI: https://doi.org/10.1007/s00216-006-0708-7