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Recent advances in capillary electrophoretic analysis of individual cells

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Abstract

Because variability exists within populations of cells, single-cell analysis has become increasingly important for probing complex cellular environments. Capillary electrophoresis (CE) is an excellent technique for identifying and quantifying the contents of single cells owing to its small volume requirements and fast, efficient separations with highly sensitive detection. Recent progress in both whole-cell and subcellular sampling has allowed researchers to study cellular function in the areas of neuroscience, oncology, enzymology, immunology, and gene expression.

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References

  1. Gunasekera N, Olson KJ, Musier-Forsyth K, Arriaga EA (2004) Anal Chem 76:655–662

    Article  CAS  Google Scholar 

  2. Kim WS, Ye X, Rubakhin SS, Sweedler JV (2006) Anal Chem 78:1859–1865

    Article  CAS  Google Scholar 

  3. Shoemaker GK, Lorieau J, Lau LH, Gillmor CS, Palcic MM (2005) Anal Chem 77:3132–3137

    Article  CAS  Google Scholar 

  4. Xiao H, Li X, Zou H, Yang L, Yang Y, Wang Y, Wang H, Le XC (2006) Anal Chim Acta 556:340

    Article  CAS  Google Scholar 

  5. Zabzdyr JL, Lillard SJ (2005) Electrophoresis 26:137–145

    Article  CAS  Google Scholar 

  6. Zhang H, Jin W (2006) J Chromatogr A 1104:346–351

    Article  CAS  Google Scholar 

  7. Miao H, Rubakhin SS, Sweedler JV (2003) Anal Bioanal Chem 377:1007–1013

    Article  CAS  Google Scholar 

  8. Zabzdyr JL, Lillard SJ (2001) J Chromatogr A 911:269–276

    Article  CAS  Google Scholar 

  9. Sun X, Jin W (2003) Anal Chem 75:6050–6055

    Article  CAS  Google Scholar 

  10. Wang W, Han S, Jin W (2006) J Chromatogr B Anal Technol Biomed Life Sci 831:57–62

    Article  CAS  Google Scholar 

  11. Woods LA, Gandhi PU, Ewing AG (2005) Anal Chem 77:1819–1823

    Article  CAS  Google Scholar 

  12. Woods LA, Powell PR, Paxon TL, Ewing AG (2005) Electroanalysis 17:1192–1197

    Article  CAS  Google Scholar 

  13. Hu S, Le Z, Newitt R, Aebersold R, Kraly JR, Jones M, Dovichi NJ (2003) Anal Chem 75:3502–3505

    Article  CAS  Google Scholar 

  14. Page JS, Rubakhin SS, Sweedler JV (2002) Anal Chem 74:497–503

    Article  CAS  Google Scholar 

  15. Krylov SN, Starke DA, Arriaga EA, Zhang Z, Chan NW, Palcic MM, Dovichi NJ (2000) Anal Chem 72:872–877

    Article  CAS  Google Scholar 

  16. Chen S, Lillard SJ (2001) Anal Chem 73:111–118

    Article  CAS  Google Scholar 

  17. Han F, Wang Y, Sims CE, Bachman M, Chang R, Li GP, Allbritton NL (2003) Anal Chem 75:3688–3696

    Article  CAS  Google Scholar 

  18. Sims CE, Meredith GD, Krasieva TB, Berns MW, Tromberg BJ, Allbritton NL (1998) Anal Chem 70:4570–4577

    Article  CAS  Google Scholar 

  19. Olefirowicz TM, Ewing AG (1990) Anal Chem 62:1872–1876

    Article  CAS  Google Scholar 

  20. Lu X, Huang W-H, Wang Z-L, Cheng J-K (2004) Anal Chim Acta 510:127–138

    Article  CAS  Google Scholar 

  21. Karlsson M, Nolkrantz K, Davidson MJ, Stromberg A, Ryttsen F, Akerman B, Orwar O (2000) Anal Chem 72:5857–5862

    Article  CAS  Google Scholar 

  22. Chiu DT, Lillard SJ, Scheller RH, Zare RN, Rodriguez-Cruz SE, Williams ER, Orwar O, Sandberg M, Lundqvist JA (1998) Science 279:1190–1193

    Article  CAS  Google Scholar 

  23. Liang H, Vu K, Trang TC, Shin D, Lee YE, Nguyen DC, Tromberg B, Berns MW (1997) Lasers Surg Med 21:159–165

    Article  CAS  Google Scholar 

  24. Fuller KM, Arriaga EA (2003) Curr Opin Biotechnol 14:35–41

    Article  CAS  Google Scholar 

  25. He M, Edgar JS, Jeffries GD, Lorenz RM, Shelby JP, Chiu DT (2005) Anal Chem 77:1539–1544

    Article  CAS  Google Scholar 

  26. Shelby JP, Edgar JS, Chiu DT (2005) Photochem Photobiol:994–1001

  27. Kuyper CL, Chiu DT (2002) Appl Spectrosc 56:300A–312A

    Article  CAS  Google Scholar 

  28. Block SM (1992) Nature 360:493–495

    Article  CAS  Google Scholar 

  29. Fuller KM, Arriaga EA (2003) Anal Chem 75:2123–2130

    Article  CAS  Google Scholar 

  30. Wallingford RA, Ewing AG (1988) Anal Chem 60:1972–1975

    Article  CAS  Google Scholar 

  31. Kennedy RT, Oates MD, Cooper BR, Nickerson B, Jorgenson JW (1989) Science 246:57–63

    Article  CAS  Google Scholar 

  32. Cruz L, Moroz LL, Gillette R, Sweedler JV (1997) J Neurochem 69:110–115

    Article  CAS  Google Scholar 

  33. Moroz LL, Dahlgren RL, Boudko D, Sweedler JV, Lovell P (2005) J Inorg Biochem 99:929–939

    Article  CAS  Google Scholar 

  34. Kim WS, Dahlgren RL, Moroz LL, Sweedler JV (2002) Anal Chem 74:5614–5620

    Article  CAS  Google Scholar 

  35. Miao H, Rubakhin SS, Sweedler JV (2005) Anal Chem 77:7190–7194

    Article  CAS  Google Scholar 

  36. Miao H, Rubakhin SS, Sweedler JV (2006) J Chromatogr A 1106:56–60

    Article  CAS  Google Scholar 

  37. Quan Z, Liu YM (2003) Electrophoresis 24:1092–1096

    Article  CAS  Google Scholar 

  38. Sheeley SA, Miao H, Ewing MA, Rubakhin SS, Sweedler JV (2005) Analyst 130:1198–1203

    Article  CAS  Google Scholar 

  39. Hu S, Jiang J, Cook LM, Richards DP, Horlick L, Wong B, Dovichi NJ (2002) Electrophoresis 23:3136–3142

    Article  CAS  Google Scholar 

  40. Hu S, Le Z, Krylov S, Dovichi NJ (2003) Anal Chem 75:3495–3501

    Article  CAS  Google Scholar 

  41. Hu S, Michels DA, Fazal MA, Ratisoontorn C, Cunningham ML, Dovichi NJ (2004) Anal Chem 76:4044–4049

    Article  CAS  Google Scholar 

  42. Arkhipov SN, Berezovski M, Jitkova J, Krylov SN (2005) Cytometry A 63:41–47

    Google Scholar 

  43. Zhang H, Jin W (2004) Electrophoresis 25:1090–1095

    Article  CAS  Google Scholar 

  44. Zabzdyr JL, Lillard SJ (2001) Anal Chem 73:5771–5775

    Article  CAS  Google Scholar 

  45. Matsunaga H, Anazawa T, Yeung ES (2003) Electrophoresis 24:458–465

    Article  CAS  Google Scholar 

  46. Liu X, Ma L, Zhang JF, Lu YT (2004) J Chromatogr B Anal Technol Biomed Life Sci 808:241–247

    Article  CAS  Google Scholar 

  47. Han F, Lillard SJ (2000) Anal Chem 72:4073–4079

    Article  CAS  Google Scholar 

  48. Han F, Lillard SJ (2002) Anal Biochem 302:136–143

    Article  CAS  Google Scholar 

  49. Chiu DT (2003) Trends Anal Chem 22:528–536

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Chemistry, Pennsylvania State University, University Park, PA, USA

    Imee G. Arcibal, Michael F. Santillo & Andrew G. Ewing

Authors
  1. Imee G. Arcibal
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  2. Michael F. Santillo
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  3. Andrew G. Ewing
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Corresponding author

Correspondence to Andrew G. Ewing.

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Arcibal, I.G., Santillo, M.F. & Ewing, A.G. Recent advances in capillary electrophoretic analysis of individual cells. Anal Bioanal Chem 387, 51–57 (2007). https://doi.org/10.1007/s00216-006-0690-0

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  • DOI: https://doi.org/10.1007/s00216-006-0690-0

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