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Analytical and Bioanalytical Chemistry

, Volume 386, Issue 2, pp 249–255 | Cite as

In situ characterisation of a microorganism surface by Raman microspectroscopy: the shell of Ascaris eggs

  • Fabienne Quilès
  • Jean-Yves Balandier
  • Sandrine Capizzi-Banas
Original Paper

Abstract

Intestinal nematodes are very common human parasites and a single species, Ascaris lumbricoïdes, is estimated to infect a quarter of the world’s population. A sticky external layer covers their eggs. This work shows that Raman vibrational confocal spectroscopy is able to give information on the biochemical composition of the shell of Ascaris eggs. The biochemical localised characterisation of Ascaris eggs was performed directly on the eggs in their aqueous environment. The studied parasites came from two origins: dissections of adult females and extractions from biosolid sludges. The presence of mucopolysaccharides, proteins and chitin in the shell was demonstrated. The presence of ascaroside compounds was shown particularly via the narrow and intense bands from the organised long CH2 chains. To the best of our knowledge, this is the first time that the latter have been observed in Raman vibrational spectra of microorganisms. Hydration of the shell was different depending on the intensity of the colour of the sludge eggs. Knowledge of the biochemical structural properties of egg surfaces would be useful to understand the egg adhesion phenomena on vegetables contaminated by reused wastewater.

Keywords

Ascaris Nematodes Raman spectroscopy Mucopolysaccharide Ascarosides Surface 

Supplementary material

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Fabienne Quilès
    • 1
  • Jean-Yves Balandier
    • 1
  • Sandrine Capizzi-Banas
    • 1
  1. 1.Laboratoire de Chimie Physique et Microbiologie pour l’Environnement (LCPME)U.M.R. 7564 CNRS-Université Henri PoincaréVillers-lès-NancyFrance

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